Peach ethylene response factor PpeERF2 represses the expression of ABA biosynthesis and cell wall degradation genes during fruit ripening

Wang, Xiaobei; Zeng, Wenfang; Ding, Yifeng; Wang, Yan; Niu, Liang; Yao, Jia‐Long; Pan, Lei; Lü, Zhenhua; Cui, Guochao; Li, Guohuai; Wang, Zhiqiang

doi:10.1016/j.plantsci.2019.02.009

Cited by 78 publications

(54 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accumulating evidence has demonstrated that ERF transcription factors may play roles in lignin biosynthesis and cell wall modification (Lee et al , ; Sakamoto et al , ; Taylor‐Teeples et al , ; Wang et al , ; Wessels et al , ). By phylogenetic analysis of the ERF superfamily, ClERF4 was found to be a member of the group IIId ERFs (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…Populus ERF139 , another group III ERF, was found to suppress vessel element expansion and stimulate guaiacyl‐type lignin accumulation (Vahala et al , ; Wessels et al , ). PpeERF2 was found to bind the promoter region to a cell wall degradation gene ( PpePG1 ) and thus to regulate peach fruit ripening (Wang et al , ). It was also found that expression of group IIId and IIIe ERF transcription factors in Arabidopsis mutants lacking secondary walls results in plants with thickened cell wall characteristics of primary cell walls in the place of secondary cell walls (Sakamoto et al , ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ethylene‐responsive factor 4 is associated with the desirable rind hardness trait conferring cracking resistance in fresh fruits of watermelon

Liao

et al. 2019

Plant Biotechnology Journal

View full text Add to dashboard Cite

Summary Fruit rind plays a pivotal role in alleviating water loss and disease and particularly in cracking resistance as well as the transportability, storability and shelf‐life quality of the fruit. High susceptibility to cracking due to low rind hardness is largely responsible for severe annual yield losses of fresh fruits such as watermelon in the field and during the postharvest process. However, the candidate gene controlling the rind hardness phenotype remains unclear to date. Herein, we report, for the first time, an ethylene‐responsive transcription factor 4 (ClERF4) associated with variation in rind hardness via a combinatory genetic map with bulk segregant analysis (BSA). Strikingly, our fine‐mapping approach revealed an InDel of 11 bp and a neighbouring SNP in the ClERF4 gene on chromosome 10, conferring cracking resistance in F2 populations with variable rind hardness. Furthermore, the concomitant kompetitive/competitive allele‐specific PCR (KASP) genotyping data sets of 104 germplasm accessions strongly supported candidate ClERF4 as a causative gene associated with fruit rind hardness variability. In conclusion, our results provide new insight into the underlying mechanism controlling rind hardness, a desirable trait in fresh fruit. Moreover, the findings will further enable the molecular improvement of fruit cracking resistance in watermelon via precisely targeting the causative gene relevant to rind hardness, ClERF4.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ethylene‐responsive factor 4 is associated with the desirable rind hardness trait conferring cracking resistance in fresh fruits of watermelon

Liao

et al. 2019

Plant Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…There is multiple evidence that the plant hormone ethylene is involved in the regulation of the ripening process in climacteric eshy fruit. Upon binding to its receptors, ethylene's signal is propagated to several downstream components which in turn target promoters of many ethylene-inducible genes, directly involved in the dramatic changes that occur during the transition from mature to ripe fruit [54]. The ACC synthase (ACS) and ACC oxidase (ACO) enzymes are responsible for turning S-Adenosyl methionine (SAM) into ethylene.…”

Section: Hormone Metabolismmentioning

confidence: 99%

“…By 2015, around 1,533 TFs were identi ed in P. persica [20], however just a few have been characterized and even less have been associated to the fruit ripening process. MADS-box PrupeSEP1 (Prupe.3G249400) [103], TCP PpTCP.A2 (Prupe.1G272500) [49], homeobox PpHB.G7 (Prupe.1G416800) [104], AP2/ERF PpERF2, PpERF3 and PpERF.E2 (Prupe.5G090800, Prupe.7G194400 and Prupe.3G032300) [54,105], ARFs PpARF5 (Prupe.1G368300) [106] and EIN3-like PpEIL1 to 5 (Prupe.6G018200, Prupe.2G058400, Prupe.2G058500, Prupe.6G181600, Prupe.2G070300) [107] are among those TFs already characterized and associated with peach fruit ripening. NAC domain TFs were enriched among the TFs that targeted the genes whose respective proteins were more abundant in mature compared to ripe fruit.…”

Section: Transcription Factors Assessmentmentioning

confidence: 99%

Shotgun proteomics of peach fruit reveals major metabolic pathways associated to ripening

Nilo-Poyanco

Moraga

Benedetto

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundFruit ripening in Prunus persica melting varieties involves several physiological changes that have a direct impact on the fruit organoleptic quality and storage potential. By studying the proteomic differences between the mesocarp of mature and ripe fruit, it would be possible to highlight critical molecular processes involved in the fruit ripening.ResultsTo accomplish this goal, the proteome from mature and ripe fruit was assessed from the variety O’Henry through shotgun proteomics using 1D-gel (PAGE-SDS) as fractionation method followed by LC/MS-MS analysis. Data from the 131,435 spectra could be matched to 2,740 proteins, using the peach genome reference v1. After data pre-treatment, 1,663 proteins could be used for comparison with datasets assessed using transcriptomic approaches and for quantitative protein accumulation analysis. Close to 26% of the genes that code for the proteins assessed displayed higher expression at ripe fruit compared to other fruit developmental stages, based on published transcriptomic data. Differential accumulation analysis between mature and ripe fruit revealed that 15% of the proteins identified were modulated by the ripening process, with glycogen and isocitrate metabolism, and protein localization overrepresented in mature fruit, as well as cell wall modification in ripe fruit. Potential biomarkers for the ripening process, due to their differential accumulation and gene expression pattern, included a pectin methylesterase inhibitor, a gibbellerin 2-beta-dioxygenase, an omega-6 fatty acid desaturase, a homeobox-leucine zipper protein and an ACC oxidase. Transcription factors enriched in NAC and Myb protein domains would target preferentially the genes encoding proteins more abundant in mature and ripe fruit, respectively.ConclusionsShotgun proteomics is an unbiased approach to get deeper into the proteome allowing to detect differences in protein abundance between samples. This technique provided a resolution so that individual gene products could be identified. Many proteins likely involved in cell wall and sugar metabolism, aroma and color, change their abundance during the transition from mature to ripe fruit.

show abstract

“…Numerous studies have demonstrated the role of auxin response factors (ARFs), the main regulators of auxin, in cell division and growth during fruit development [20][21][22][23][24]. Transcription factors also regulate genes related to fruit development [8,25,26].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis of Two Bottle Gourd Accessions Differing in Fruit Size

Zhang

Tan

Zhang

et al. 2020

Genes

View full text Add to dashboard Cite

The bottle gourd (Lagenaria siceraria) is an important horticultural and medicinal crop with high nutritional value. This study aimed at examining the molecular regulation of fruit size in bottle gourd. We performed transcriptome sequencing of two bottle gourd cultivars differing in their fruit size. The average fruit length and weight of the cultivar Hang (39.48 cm/624.4 g) were higher than those of the cultivar USA (10.34 cm/152.8 g) at maturity. Transcriptome sequencing and assembly resulted in 89,347 unigenes. A total of 1250 differentially expressed genes (DEG) were found between the two cultivars, including 422 upregulated genes and 828 downregulated genes in Hang as compared to USA. Genes related to cell wall metabolism, phytohormones, cell cycle, and cell division showed significant differential expression between the two cultivars. DEGs encoding transcription factors (TF) from nine TF families were also identified. The ethylene response factor family was the most enriched among these families. Our study provides a basis for further investigations of the molecular regulation of fruit size in bottle gourd.

show abstract

Peach ethylene response factor PpeERF2 represses the expression of ABA biosynthesis and cell wall degradation genes during fruit ripening

Cited by 78 publications

References 42 publications

Ethylene‐responsive factor 4 is associated with the desirable rind hardness trait conferring cracking resistance in fresh fruits of watermelon

Ethylene‐responsive factor 4 is associated with the desirable rind hardness trait conferring cracking resistance in fresh fruits of watermelon

Shotgun proteomics of peach fruit reveals major metabolic pathways associated to ripening

Comparative Transcriptomic Analysis of Two Bottle Gourd Accessions Differing in Fruit Size

Contact Info

Product

Resources

About