Pectin Hydrogels Ph Stability as Affected by Methacrylic Grafting to Low Methoxy Pectin Structure

Cornejo-Ramírez, Yaeel I.; Carvajal–Millán, Elizabeth; Brown, Francisco; Sánchez-Villegas, J.A.; Rascón-Chu, A.

doi:10.24275/uam/izt/dcbi/revmexingquim/2019v18n2/cornejor

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Glycidyl methacrylate modified pectin has been commonly applied to synthesize water-insoluble hydrogels that appeared alternative support matrices for β- d -galactosidase immobilization because of elevated mechanical strength, and thus protect the enzyme molecule during hydrolytic reactions. Such hydrogels have also been used to immobilize urease [ 26 , 27 , 28 ]. Cargnin et al [ 29 ] explored the potential of pinus residue/pectin-based bio-composite hydrogel matrices to immobilize β- d -galactosidase.…”

Section: Hydrogels For Biocatalysismentioning

confidence: 99%

Expanding the Biocatalytic Scope of Enzyme-Loaded Polymeric Hydrogels

Tan

Bilal

Raza

et al. 2021

Gels

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In recent years, polymeric hydrogels have appeared promising matrices for enzyme immobilization to design, signify and expand bio-catalysis engineering. Therefore, the development and deployment of polymeric supports in the form of hydrogels and other robust geometries are continuously growing to green the twenty-first-century bio-catalysis. Furthermore, adequately fabricated polymeric hydrogel materials offer numerous advantages that shield pristine enzymes from denaturation under harsh reaction environments. For instance, cross-linking modulation of hydrogels, distinct rheological behavior, tunable surface entities along with elasticity and mesh size, larger surface-volume area, and hydrogels’ mechanical cushioning attributes are of supreme interest makes them the ideal candidate for enzyme immobilization. Furthermore, suitable coordination of polymeric hydrogels with requisite enzyme fraction enables pronounced loading, elevated biocatalytic activity, and exceptional stability. Additionally, the unique catalytic harmony of enzyme-loaded polymeric hydrogels offers numerous applications, such as hydrogels as immobilization matrix, bio-catalysis, sensing, detection and monitoring, tissue engineering, wound healing, and drug delivery applications. In this review, we spotlight the applied perspective of enzyme-loaded polymeric hydrogels with recent and relevant examples. The work also signifies the combined use of multienzyme systems and the future directions that should be attempted in this field.

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Section: Hydrogels For Biocatalysismentioning

confidence: 99%

Expanding the Biocatalytic Scope of Enzyme-Loaded Polymeric Hydrogels

Tan

Bilal

Raza

et al. 2021

Gels

View full text Add to dashboard Cite

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“…The physical variables monitored for the superficial water quality evaluation and that were measured in situ with the use of a multiparameter meter brand HQd Portable were: temperature, turbidity, dissolved oxygen, pH, conductivity and biochemical oxygen demand (BOD) (8,10,14,21).…”

Section: Physical Characterizationmentioning

confidence: 99%

First study of water quality in the San Camilo and Mojahuevo estuaries located in Guayas for being used in aquaculture

et al. 2021

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The water quality of the San Camilo and Mojahuevo estuaries was monitored in the months of April (rainy season) and June (dry season) of 2016. The water of the Mojahuevo estury presented in both seasons the lowest values of turbidity between 17.20 and 33.70 UNT, biochemical oxygen demand being in a range between 14.00 and 19.00 mg/L, zinc content (0.02-0.05 mg/L) and copper content (0.07 mg/L), ammonia content (0.21-0.35 mg/L), fats and oils (0.23-0.29 mg/L), phosphates (0.39-2.34 mg/L) and nitrates (0.84-2.29 mg/L), while the water of the two estuaries San Camilo and Mojahuevo (rainy season) showed similar phenolic content (0.019 mg/L). The results showed that the Mojahuevo estuary presented the high quality of water recommended for being used in productive process like aquaculture due to the low presence of pollutants.

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High‐Throughput Assessments for Storage Stability, In Vitro Release, and Particle Size of Encapsulated Biocontrol Fungi in Hydrogel Beads

2022

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Use of biocontrol fungi (BF) such as Trichoderma spp. minimizes fungicide input and increases both plant nutrition and protection from disease. Thus, the introduction of BF by novel inoculants in crop management is an excellent strategy to promote sustainable antagonism activity. Within these strategies, encapsulation in polymeric matrices such as hydrogel beads will play a prominent role in providing an effective carrier/protector and long-lasting bioproduct. These studies have used biomaterials with tunable physicochemical features, providing differential morphologies, compaction, and disintegration, among other parameters. Aiming at developing bioproducts within polymeric hydrogel beads, viability of encapsulated conidia, storage stability, release of active ingredient, and particle size are essential. However, there are no reports that detail standardized and comprehensive methods to evaluate the characterization of these bioproducts. We describe step-by-step protocols that go from sample preparation to testing the viability and storage stability using vacuumsealed aluminum foil bags. We also describe a high-throughput in vitro method for quantifying released fungal conidia of BF at different pH values. Finally, the particle size of beads is established by bright-field microscopy. These protocols could be transferable to other biological actives, accessible to researchers in the microbiology and bioengineering communities.

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Pectin Hydrogels Ph Stability as Affected by Methacrylic Grafting to Low Methoxy Pectin Structure

Abstract: Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)

Cited by 5 publications

References 39 publications

Expanding the Biocatalytic Scope of Enzyme-Loaded Polymeric Hydrogels

Expanding the Biocatalytic Scope of Enzyme-Loaded Polymeric Hydrogels

First study of water quality in the San Camilo and Mojahuevo estuaries located in Guayas for being used in aquaculture

High‐Throughput Assessments for Storage Stability, In Vitro Release, and Particle Size of Encapsulated Biocontrol Fungi in Hydrogel Beads

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