Peer Reviewed: Capillary Electrophoresis and Daubert: Time for Admission

Kuffner, C. A.; Marchi, E.; Morgado, J. M.; Rubio, Chris

doi:10.1021/ac9618839

Cited by 20 publications

(9 citation statements)

References 1 publication

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“…Together, these results indicate that sizing measurements made using CAE are both consistent with and as good as those reported by expert laboratories in the field. In addition, the use of software and reference DNA standards to normalize sequence-specific measurement errors should enable a more direct comparison of genetic typing results between laboratories or for computation of test results in a forensic context (Kuffner et al 1996). Knowles et al (1992) first proposed a method for interlaboratory standardization of linkage results using CEPH DNA typing standards.…”

Section: Sizing Accuracy and Precisionmentioning

confidence: 99%

Sensitivity, reproducibility, and accuracy in short tandem repeat genotyping using capillary array electrophoresis.

Mansfield¹,

Vainer²,

Enad³

et al. 1996

Genome Res.

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The Human Genome Initiative has increased significantly the rate at which disease-causing genes are being mapped and sequenced. New cost-effective methods to locate the genes and to characterize disease-causing mutations require robust, reproducible, and accurate protocols for measuring DNA fragment lengths. Capillary array electrophoresis (CAE) offers rapid, high-resolution separations, high throughput, and sensitive detection. To assess the utility of CAE for the accumulation of genetic information, we tested both sizing accuracy and reproducibility using 48-capillary prototype systems. Two multiplex PCR allelic ladder standards and several CA-repeat markers were analyzed in >100 runs. Reproducibility in typing >8000 genotypes reveals a standard deviation of less than 0.2 bp on these systems under optimized conditions. However, sequence-dependent migration anomalies were observed at most simple sequence loci even when analyzed under denaturing conditions, resulting in a systematic bias in estimated fragment sizes. We show here that, by normalizing results to known typing controls, one can obtain locus-averaged accuracies of <0.06 bp and normalized results within I bp of actual. We detect as little as a 1:30,000 dilution of a DNA quantitation standard stained with highly sensitive intercalating dyes, indicating an 80-zeptomole sensitivity limit. However, to obtain reproducible electrokinetic injection, -200 attomoles of fluorescein-labeled DNA is required. These sensitivity limits, sizing precision, and accuracy, together with the I-hr run times for 48-96 samples, indicate that CAE is a viable method for high-throughput genetic analysis of simple sequence repeat polymorphisms.

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Section: Sizing Accuracy and Precisionmentioning

confidence: 99%

Sensitivity, reproducibility, and accuracy in short tandem repeat genotyping using capillary array electrophoresis.

Mansfield¹,

Vainer²,

Enad³

et al. 1996

Genome Res.

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“…Capillary electrophoresis is an acceptable separation technique under SWGDRUG recommendations. In fact, the admissibility of CE in court was the subject of a 1996 publication in which the authors used CE as an example to show how a new science may meet the requirements of the Federal Rules of Evidence introduced by the Daubert Standard, a legal http://precedent set in http://1993 by the http://Supreme Court of the http://United States regarding the admissibility of http://expert witnesses' http://testimony during legal proceedings . In the past, applications of capillary electrophoresis to drug analysis in forensic laboratory even at Federal levels was mainly limited to enantiomeric separation of chiral illicit substances that were scheduled drugs such as methamphetamine, in which the sentencing depends on the chiral identification of illicit drugs.…”

Section: Applications Of Liquid Separation Ms To the Analysis Of Illimentioning

confidence: 99%

Applications of liquid‐based separation in conjunction with mass spectrometry to the analysis of forensic evidence

Moini

2018

Electrophoresis

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In the past few years, there has been a significant effort by the forensic science community to develop new scientific techniques for the analysis of forensic evidence. Forensic chemists have been spearheaded to develop information-rich confirmatory technologies and techniques and apply them to a broad array of forensic challenges. The purpose of these confirmatory techniques is to provide alternatives to presumptive techniques that rely on data such as color changes, pattern matching, or retention time alone, which are prone to more false positives. To this end, the application of separation techniques in conjunction with mass spectrometry has played an important role in the analysis of forensic evidence. Moreover, in the past few years the role of liquid separation techniques, such as liquid chromatography and capillary electrophoresis in conjunction with mass spectrometry, has gained significant tractions and have been applied to a wide range of chemicals, from small molecules such as drugs and explosives, to large molecules such as proteins. For example, proteomics and peptidomics have been used for identification of humans, organs, and bodily fluids. A wide range of HPLC techniques including reversed phase, hydrophilic interaction, mixed-mode, supercritical fluid, multidimensional chromatography, and nanoLC, as well as several modes of capillary electrophoresis mass spectrometry, including capillary zone electrophoresis, partial filling, full filling, and micellar electrokenetic chromatography have been applied to the analysis drugs, explosives, and questioned documents. In this article, we review recent (2015-2017) applications of liquid separation in conjunction with mass spectrometry to the analysis of forensic evidence.

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“…CE has been shown to be a versatile technique and has been used for a variety of forensic applications including analysis of gunshot residues, explosive residues, and drugs as well as DNA typing [13]. Since 1996, CE results have been admissible in courts of law [14].…”

Section: Early Work With Cementioning

confidence: 99%

Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis

et al. 2004

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DNA typing with short tandem repeat (STR) markers is now widely used for a variety of applications including human identification. Capillary electrophoresis (CE) instruments, such as the ABI Prism 310 and ABI 3100 Genetic Analyzers, are the method of choice for many laboratories performing STR analysis. This review discusses issues surrounding sample preparation, injection, separation, detection, and interpretation of STR results using CE systems. Requirements for accurate typing of STR alleles are considered in the context of what future analysis platforms will need to increase sample throughput and ease of use.

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Peer Reviewed: Capillary Electrophoresis and Daubert: Time for Admission

Cited by 20 publications

References 1 publication

Sensitivity, reproducibility, and accuracy in short tandem repeat genotyping using capillary array electrophoresis.

Sensitivity, reproducibility, and accuracy in short tandem repeat genotyping using capillary array electrophoresis.

Applications of liquid‐based separation in conjunction with mass spectrometry to the analysis of forensic evidence

Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis

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