Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales

Abstract

A Gram-negative, chemoheterotrophic, facultatively anaerobic, slightly halophilic, oval-shaped marine bacterium, designated HTCC2601 T , was isolated from the western Sargasso Sea by high-throughput culturing involving dilution to extinction. Although the 16S rRNA gene sequence similarity between the isolate and Salipiger mucosus was 96?5 %, phylogenetic analyses using different treeing algorithms clearly indicated that the strain forms a distinct lineage within a clade containing the recently classified genera Salipiger and Palleronia in the order Rhodobacterales of the Alphaproteobacteria. The DNA-DNA relatedness between strain HTCC2601 T and S. mucosus was 26?3 %. Strain HTCC2601 T utilized a wide range of carbohydrates, including hexose monomers, sugar alcohols, organic acids and amino acids, as sole carbon sources. The DNA G+C content of strain HTCC2601 T was 65?4 mol%, and the predominant constituents of the cellular fatty acids were 18 : 1v7c (79?7 %) and 11-methyl 18 : 1v7c (7?5 %). The strain differed from members of the closely related genera Salipiger and Palleronia in its morphological, biochemical and ecological characteristics. On the basis of the taxonomic data obtained in this study, a novel genus and species, Pelagibaca bermudensis gen. nov., sp. nov., is proposed; HTCC2601 T (=KCTC 12554 T =JCM 13377 T ) is the type strain of Pelagibaca bermudensis.

Marivita cryptomonadis gen. nov., sp. nov. and Marivita litorea sp. nov., of the family Rhodobacteraceae, isolated from marine habitats

“…Positive for the Table 1. Selected differential characteristics between strains CL-SK44 T and CL-JM1 T and related taxa in the family Rhodobacteraceae Taxa: 1, strain CL-SK44 T ; 2, strain CL-JM1 T ; 3, Thalassobius gelatinovorans (data from Rüger & Höfle, 1992;Arahal et al, 2005); 4, Thalassobius aestuarii (Yi & Chun, 2006); 5, Thalassobius mediterraneus (Arahal et al, 2005); 6, Donghicola eburneus (Yoon et al, 2007a); 7, Oceanicola (Cho & Giovannoni, 2004;Gu et al, 2007;Lin et al, 2007); 8, Sulfitobacter (Pukall et al, 1999;Labrenz et al, 2000;Ivanova et al, 2004;Park et al, 2007;Yoon et al, 2007b); 9, Pelagibaca bermudensis (Cho & Giovannoni, 2006); 10, Marinovum algicola (Lafay et al, 1995;Martens et al, 2006) (Yi & Chun, 2006). DAL, an unidentified aminolipid; DPG, diphosphatidylglycerol; GL, an unidentified glycolipid; L, an unidentified lipid PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL, an unidentified phospholipid.…”

Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment

“…The number of the genera belonging to the Roseobacter clade has increased considerably through the continual descriptions of novel genera and reclassifications (Euzéby, 1997). Recently, genera exhibiting relatively high 16S rRNA gene sequence similarities of more than 96 % to the representative(s) of the Roseobacter clade, irrespective of their phylogenetic positions, have been described (Cho & Giovannoni, 2006;Kim et al, 2008;Hwang et al, 2009;Vandecandelaere et al, 2009;Romanenko et al, 2011;Hahnke et al, 2013;Yoon et al, 2013b). In this study, we describe one novel strain (designated BS-B2 T ) that was isolated from a tidal flat of the southern coast of the Korean peninsula, in which cockles (Tegillarca granosa) are known to inhabit in large numbers.…”

Lewinella antarctica sp. nov., a marine bacterium isolated from Antarctic seawater

“…Phylogenetic analyses, including multiple alignment of 16S rRNA gene sequences, determination of sequence similarity and generation of phylogenetic trees, were performed using the ARB package (Ludwig et al, 2004) and PAUP* (Swofford, 2002) as described previously (Cho & Giovannoni, 2006). Sequence comparisons in the ARB database and BLASTN hits from GenBank showed that strain IMCC3223 T was closely related to members of the genus Lewinella.…”