Penconazole and calcium ameliorate drought stress in canola by upregulating the antioxidative enzymes

Abstract

The aim of this research was to gauge the alternations in the lipid peroxidation and antioxidative enzyme activity in two cultivars (cv. RGS003 and cv. Sarigol) of canola under drought stress and drought tolerance amelioration by penconazole (PEN) and calcium (Ca). Plants were treated with different polyethylene glycol (PEG) concentrations (0, 5, 10 and 15%) without or with PEN (15 mg L–1) and Ca (15 mM). The Ca treatment prevented the negative effects of drought on fresh weight (FW) in RGS003 and Sarigol at 5 and 15% PEG respectively. Ca and PEN/Ca treatments caused significant induction in the proline content in Sarigol at 15% PEG; the latter treatment was accompanied by higher glycine betaine (GB), lower malondialdehyde (MDA) and growth recovery. Hydrogen peroxide (HO2) content in Sarigol was proportional to the severity of drought stress and all PEN, Ca and PEN/Ca treatments significantly reduced the H2O2 content. PEN and PEN/Ca caused alleviation of the drought-induced oxidative stress in RGS003. RGS003 cultivar exhibited significantly higher antioxidative enzymes activity at most levels of drought, which could lead to its drought tolerance and lower MDA content. In contrast to that of Sarigol, the activity of catalase and superoxide dismutase (SOD) increased with Ca and PEN/Ca treatments in RGS003 under low stress. The application of PEN and Ca induced significantly P5CS and SOD expression in RGS003 under drought stress after 24 h. Overall, these data demonstrated that PEN and Ca have the ability to enhance the tolerance against the drought stress in canola plants.

Overexpression of a Malus baccata MYB Transcription Factor Gene MbMYB4 Increases Cold and Drought Tolerance in Arabidopsis thaliana

“…Due to the high level of ROS in the body when plants are dehydrated, which causes membrane damage, protective enzymes such as SOD, POD, and CAT begin to work to remove excessive reactive oxygen species to maintain intracellular balance. At the same time, studies have found that the activities of POD and CAT in the root of cold-acclimated rice are significantly enhanced, indicating that these antioxidant enzymes also play the most important role in cold resistance [40]. The results of the study showed that, compared with WT and UL A. thaliana, the activity of CAT and POD in transgenic A. thaliana with MbMYB4 was significantly increased, and the content of MDA was significantly reduced.…”

RNA-Seq Analysis Demonstrates Different Strategies Employed by Tiger Nuts (Cyperus esculentus L.) in Response to Drought Stress

“…Drought stress limits the growth, development and survival rate of plants, leading to tremendous losses in yield, thus threatening global agricultural production [ 39 ]. In our study, 15% PEG was used to simulate drought stress [ 29 , 40 , 41 , 42 ]. Therefore, the analysis of the response of sprouts to drought stress at the bud stage is important for actual agricultural production [ 43 ].…”

“…The consistent growth sprouts were selected as plant materials for further experiments. A 15% PEG concentration (the solvent was distilled water) was used to simulate drought stress (D), while distilled water treatment was set as control treatment (CK) [ 29 ]. The samples were collected after 0 h, 3 h, 6 h, 12 h, 24 h and 48 h of treatment, respectively.…”

Genome-wide analysis of MYB transcription factors of Vaccinium corymbosum and their positive responses to drought stress

“…For instance, in Manihotesculenta, the SOD activity of leaves increased significantly under drought stress [60]. In Brassica campestris, SOD activity and the content of H 2 O 2 increased obviously when plants were subjected to drought stress [61]. Nevertheless, further study are needed for the 10 key VcMYB DEGs we screened to reveal the accurate signaling pathways of its involvement.…”