This study aimed to determine the effect of video education about hypoglycemia on knowledge of diabetes mellitus patients and their families. This type of research is a quantitative study with a pre-experiment method. This study focused on two groups, namely, type 2 diabetes mellitus patients by 162 people, and the families of diabetes mellitus patients by 162 people. In the group of diabetes mellitus patients, the sample used 30 respondents who were divided into two groups, namely 15 respondents in the experimental group and 15 respondents in the control group. In the population of family diabetes mellitus patients, the sample applied 30 respondents, who were divided into 15 respondents for the experimental group and 15 respondents for the control group. Non-probability sampling with a purposive sampling technique was utilized. Data analysis using the paired t-test showed a p-value of 0.001 <0.05. So, it can be concluded that health education on knowledge of hypoglycemia in type 2 diabetes mellitus patients was effective in the working area of Puskesmas Nguter Sukoharjo. For the results of the paired t-test, we found p = <0.001 in the experimental group, which showed that there was an effect of hypoglycemia health education with videos on knowledge of type 2 diabetes families. Diabetes mellitus patients need to always adopt a healthy lifestyle so as to create a good health status and avoid complications of diabetes mellitus patients.