Allium sativum L., or fermented single garlic, is an garlic that is heated for 14 days at 70°C with a humidity of 70–80% to turn it black. In this study, the total phenolic content and antioxidant activity of an ethanol extract from a single black garlic (Allium sativum L) are to be determined. Maceration is used to extract a single black garlic (Allium sativum L), and the folin-ciocalteau method is used to measure total phenolics. Using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) technique, antioxidants were evaluated. The results of the study demonstrate that the ethanol extract of a single black garlic (Allium sativum L) has a total phenolic content of 62,45% ± 0,2886 and that the extract's antioxidant activity has an IC50 value of 10.4156 μg/mL (very strong antioxidant). The high phenolic content and antioxidant activity which is classified as very strong indicate that single black garlic ethanol extract has potential for oral and dental health.