Pengaruh Fraksi Ekstrak Daun Pacar Cina (Aglaia Odorata L.) Terhadap Pertumbuhan Colletotrichum Capsici Penyebab Penyakit Antraknosa Pada Cabai (Capsicum Annuum L.) Secara in Vitro

Abstract

Influence of Aglaia odorata L. leaf extract on in vitro growth of chilli antrachnose fungi (Colletotrichum capsici). Pacar cina (Aglaia odorata L.) is known as one of the potential herbs as fungicides. The objective of this study was to determine the effectiveness of various leaf extract fractions of pacar cina as a fungicide to suppress the in vitro growth of C. capsici, the causal agent of anthracnose disease on chili. This study consisted of two sub-experiments. The first experiment consisted of 12 treatments: leaf extract fractions with distilled water solvent, leaf extract fractions with ethanol solvent with a concentration of 10, 20, 30, 40, 50, 60, 70, 80 and 90%, propineb 0.2 % (synthetic fungicides) and control (without fungicide). The second experiment consisted of 12 treatments: leaf extract fractions with distilled water solvent, leaf extract fractions with the solvent of n-hexane at a concentration of 10, 20, 30, 40, 50, 60, 70, 80 and 90%, propineb 2% (synthetic fungicides), and control (without fungicides). The treatmentswerearranged by completely randomized design (CRD) with three replications. Variables measured in this experiment were colony diameter and density of spores of C. capsici. Data were analyzed by analysis of variance (ANOVA) and differences between the mean values was tested by Duncan Multiple Range Test (DMRT) with the level of 5% significant different. Additionally, leaf extract that effectively inhibited the growth of C. capsici was the fraction of the leaf extract with 80% ethanol solvent and 10%, 60%, and 90% n-hexanesolvent. The result showed that the leaf extract fraction of pacar cina was potentially used as fungicide to inhibit the growth of C. capsici.Key words: Aglaia odorata L., anthracnose, Capsicum annuum L., Colletotrichum capsici, growth inhibition ABSTRAK Pengaruh fraksi ekstrak daun pacar cina (Aglaia odorata L.) terhadap penghambatan pertumbuhan Colletotrichum capsici penyebab penyakit antraknosa pada cabai (Capsicum annuum L.) secara in vitro. Pacar cina merupakan salah satu tumbuhan yang berpotensi sebagai fungisida nabati. Penelitian ini bertujuan untuk mengetahui efektivitas berbagai fraksi ekstrak daun pacar cina sebagai fungisida nabati dalam menekan pertumbuhan C. capsici penyebab penyakit antraknosa pada cabai secara in vitro. Penelitian terdiri dari dua sub percobaan. Percobaan pertama terdiri dari 12 perlakuan yaitu, ekstrak daun pacar cina dari fraksi pelarut aquades, pelarut alkohol dengan konsentrasi 10, 20, 30, 40, 50, 60, 70, 80, dan 90%, propineb (fungisida sintetik) dan kontrol (tanpa fungisida). Percobaan kedua terdiri dari 12 perlakuan yaitu ekstrak daun pacar cina dari fraksi pelarut aquades, pelarut n-hexana dengan konsentrasi 10%, 20%, 30, 40%, 50%, 60%, 70%, 80%, dan 90%, fungisida propineb, dan kontrol. Perlakuan dalam percobaan disusun dalam Rancangan Acak Lengkap (RAL) dengan tiga ulangan. Peubah yang diamati adalah pertumbuhan diameter koloni dan kerapatan spora C. capsici. Data yang diperoleh dianalisis dengan sidik ragam (Anov...