Abstract:Penelitian ini bertujuan untuk mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium kerbau terhadap kualitas oosit dan tingkat maturasi oosit secara in vitro. Sebanyak 30 ovarium yang berasal dari RPH kemudian dikelompokkan berdasarkan keberadaan CL. Setelah itu oosit dikoleksi secara slicing, oosit yang diperoleh dikelompokkan berdasarkan kekompakan sel kumulus dan status sitoplasma. Maturasi pada medium Tissue Culture Medium-199 (TCM-199) yang ditambahkan Penstrep, Pregnant Mare Serum Gonadotrophin… Show more
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