PENGARUH METODE EKSTRAKSI DAN POLARITAS PELARUT TERHADAP  KADAR FENOLIK TOTAL DAUN KERSEN (Muntingia calabura L)

Yulianti, Wina; Ayuningtyas, Gilang; Martini, Rina; Resmeiliana, Ika

doi:10.29244/jstsv.10.2.41-49

Cited by 9 publications

(11 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each plant material is extracted by cold method, namely the maceration method, because it is easier and the chemical compounds are not damaged [19]. The solvent used is 70% ethanol because of its general and can extract a wide range of polarity from polar and nonpolar compounds, more quickly and safely and can inhibit the growth of other microbes [20,21]. The yield obtained for A. paniculata extract was 11.2%, and for T. crispa was 19.1%.…”

Section: Resultsmentioning

confidence: 99%

Protein-Protein Interaction Analysis to Identify Nuclear Factor-Erythroid-2 Factor 2 (Nrf2) Inhibition by Extracellular Enzymes From Water Kefir Organisms

ZUHROTUN,

PRIJADI,

INDRADI

et al. 2023

Int J App Pharm

View full text Add to dashboard Cite

Objective: This research was conducted to screen the anticancer activity of bitter herbs that contains Andrographis paniculata (Brum. f) leaves (AP) and Tinospora crispa L. stems (TC) in form of fresh materials and extracts using a mechanism-based yeast bioassay. Methods: Samples tested by mechanism-based yeast bioassay (MBYB) were single extract, mixed extract, and jamu gendong pahitan from a traditional market and made in the laboratory. Fresh sample of jamu gendong pahitan from the market and a single extract (AP and TC) was tested at one dose. While fresh jamu gendong pahitan made in the laboratory and the mixed extract (AP: TC) was tested at three different doses, doses 1 (3:10), dose 2 (1:1), and dose 3 (10:3). The leaves and stems were extracted by 70% ethanol for 3x24 h, each day the solvent was changed then every macerate was evaporated using a rotavapor and water bath. By this MBYB method, noted that the active sample must have an IC12 value of<8000µg/ml, so all the samples or doses were tested using final concentration varying at around 8,000; 4,000; 2,000; 1,000; 500, 250, and 125µg/ml. Results: The percentage yield of Andrographis paniculata (Brum. f) leaves was 11.2% and Tinospora crispa L. stems was 19.%. The activity assay for jamu gendong pahitan from the traditional market was inactive as a topoisomerase inhibitor (IC12>8000µg/ml). Samples showed topoisomerase I inhibitor activity were jamu gendong pahitan made in laboratory doses 1 and 2. While samples showed topoisomerase I and II inhibitor activities were jamu gendong pahitan made in laboratory dose 3, single and mixed extracts. Conclusion: The fresh material of jamu gendong pahitan (bitter herbs) bought from the market is inactive, while the fresh material of samples of jamu gendong pahitan made in laboratory doses 1 and 2 have topoisomerase I inhibitor activity. Based on the IC12, value, it is known that the sample that gave the best activity was the mixed extract of bitter herbs dose 3 that contain extract of A. paniculata and T. crispa (10:3), with IC12 values in strains 1138, 1140, and 1353 were 926.28±173, 576.75±42, and 865.5±135µg/ml respectively.

show abstract

Section: Resultsmentioning

confidence: 99%

Protein-Protein Interaction Analysis to Identify Nuclear Factor-Erythroid-2 Factor 2 (Nrf2) Inhibition by Extracellular Enzymes From Water Kefir Organisms

ZUHROTUN,

PRIJADI,

INDRADI

et al. 2023

Int J App Pharm

View full text Add to dashboard Cite

show abstract

“…microcarpum L. yang diekstraksi dengan etanol 96% dalam penangas air suhu 40 selama 5 jam dan setelahnya dibuat enam pengenceran untuk perlakuan pada tanaman. Etanol merupakan salah satu pelarut dengan karakteristik polar yang sangat baik untuk proses ekstraksi (Yulianti et al, 2020). Pelarut etanol ini dapat lebih cepat menarik bahan kimia bioaktif karena dapat menembus bahan dinding sel (Prayitno & Rahim, 2020, dalam Yulianti et al, 2020.…”

Section: Hasil Dan Diskusiunclassified

Kandungan senyawa capsaicin dalam cabai (Capsicum Annuum L) sebagai anti hama pada sayuran: Kajian pustaka

Risma Khoerunisa,

Risya Jheniar Nur Siti Khamilah,

Dzuhrika Gantari Ayuanindya

et al. 2024

HJTAS

View full text Add to dashboard Cite

Vegetables are one of the most widely cultivated crops because of their important role in fulfilling food nutrition. In the implementation of vegetable cultivation, there will be obstacles such as pest and disease attacks. Chili plants can be used as vegetable pesticides because they have capsaicin compounds that can have a negative effect on pests. This literature review aims to discuss capsaicin compounds as anti-pests in vegetables, namely by causing the death of vegetable pests in several studies. The method used was a literature study. The results in the four articles reviewed show that the capsaicin compound in chili peppers can be used as a vegetable pesticide because it can affect digestion, inhibit growth and nerve work in pests which ultimately causes the death of these pests. The higher the concentration of chili extract, the higher the percentage of pest mortality. This vegetable pesticide from red chili extract can be used as an alternative to chemical pesticides.

show abstract

“…Methanol is a solvent that is polar and able to dissolve polar compounds so it is very well used to extract secondary metabolite compounds contained in the sample (Khasanah et al, 2021). Ethanol is a polar solvent with properties that allow it to penetrate cell walls, so it is able to perform cell diffusion and attract bioactive compounds more quickly (Yulianti et al, 2020). In addition, ethyl acetate is a low-toxicity solvent that is semi-polar, has the ability to attract polar and nonpolar compounds (Zainudin et al, 2018).…”

Section: Sample Extractionmentioning

confidence: 99%

“…Ethyl acetate is a semi-polar solvent with the ability to extract both polar and nonpolar compounds (Zainudin et al, 2018). Meanwhile, ethanol has properties that allow it to penetrate cell walls, enabling cell diffusion and faster extraction of bioactive compounds (Yulianti et al, 2020). On the other hand, methanol has an advantage in dissolving polar compounds, such as phenols (Yuliarni et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Different Solvents on Total Tannin Content of Cengkodok (Melastoma malabathricum) Leaf Extracts

Masriani,

Parawansa,

Sasri

et al. 2023

HJKK

View full text Add to dashboard Cite

The use of synthetic dyes as color enhancers or generators has caused various environmental and health problems. Plant tannin biomordants offer an alternative solution to address these issues. The sample used in this study were Cengkodok (M. malabathricum) plants that grow wild in places that get enough sunlight such as shrubs. Phytochemical screening on various parts of the cengkodok plant revealed that the highest tannin content is found in the leaf. However, until now there has been no scientific evidence that presents differences in tannin levels in cengkodok leaf with various types of solvents. This study aims to determine the yield and total tannin content of cengkodok leaf extract obtained through maceration process using methanol, ethanol, and ethyl acetate solvents. The extract yield was determined by comparing the weight of the dry extract with the powdered cengkodok leaf material. The total tannin content was analyzed using UV-Vis spectroscopy at a wavelength of 755.8 nm with Folin-Ciocalteu reagent and sodium carbonate. Tannic acid was used as a reference. The research results showed that the yield values for Cengkodok leaf extracted with methanol, ethanol, and ethyl acetate were 5.05%, 4.72%, and 1.59%, respectively. The total tannin content of cengkodok leaf extracts with methanol, ethanol, and ethyl acetate solvents was 0.47%±0.04, 0.37%±0.01, and 0.19%±0.04, respectively. Based on this research, it can be concluded that methanol is the most effective solvent in extraction and determination of total tannin content of Cengkodok leaf extracts.

show abstract

PENGARUH METODE EKSTRAKSI DAN POLARITAS PELARUT TERHADAP KADAR FENOLIK TOTAL DAUN KERSEN (Muntingia calabura L)

Cited by 9 publications

References 12 publications

Protein-Protein Interaction Analysis to Identify Nuclear Factor-Erythroid-2 Factor 2 (Nrf2) Inhibition by Extracellular Enzymes From Water Kefir Organisms

Protein-Protein Interaction Analysis to Identify Nuclear Factor-Erythroid-2 Factor 2 (Nrf2) Inhibition by Extracellular Enzymes From Water Kefir Organisms

Kandungan senyawa capsaicin dalam cabai (Capsicum Annuum L) sebagai anti hama pada sayuran: Kajian pustaka

The Effect of Different Solvents on Total Tannin Content of Cengkodok (Melastoma malabathricum) Leaf Extracts

Contact Info

Product

Resources

About