Vegetable fern species distributed throughout the world are diverse, but there is a lack of scientific data about the nutritional content of local vegetable ferns. This study aimed to provide preliminary data of Diplazium esculentum nutrition obtained from Bone Bolango District, Gorontalo area, Indonesia, in order to support the developing of pharmaceutical and mixed animal feed products. Tests for the proximate characteristics of leaf extracts and the phytochemical analysis were carried out through qualitative screening following Indonesian National Standard (SNI) Method year 2006, while the detail amino acid compound analyzed using Gas Chromatography Mass Spectrometry (GCMS) method. The results showed that D. esculentum from Gorontalo Land is rich with proteins and amino acids. The amount of protein and fat is detected as 21.52 ± 2.70% and 2.47 ± 0.97% respectively. The fern also contains complete secondary metabolites in the form of alkaloids, saponins, tannins, phenolics, flavonoids, triterpenoids, steroids, and glycosides with a high amount.