Pengaruh Waktu Inkubasi Pada Proses Sexing Sperma Berbasis Glutathione Terhadap Motilitas Dan Membran Plasma Utuh Chilled Semen Domba Lokal

Abstract

AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh waktu inkubasi pada proses sexing sperma berbasis glutathione terhadap motilitas dan membran plasma untuh (MPU) chilled semen domba lokal. Proses pemisahan sperma X dan Y dilakukan dengan menggunakan metode kolom bovine serum albumin (BSA). Penelitian ini merupakan penelitian eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan tiga perlakuan yaitu, 45 menit (T1), 60 menit (T2) dan 75 menit (T3). Data dianalisis menggunakan analisis ragam dan diikuti dengan uji lanjut berganda Duncan. Materi yang digunakan adalah semen segar domba lokal dengan motilitas sperma ≥ 70% dan glutathione sebanyak 5 mM. Hasil penelitian menunjukan bahwa persentase motilitas tertinggi pada fraksi atas (sperma X) dan fraksi bawah (sperma Y) terdapat pada perlakuan T1 (69,73% dan 68,82%) diikuti oleh T2 (66,40% dan 64,55%) dan T3 (57,90% dan 57,65%). Selain itu, hasil penelitian menunjukan bahwa persentase MPU sperma tertinggi pada fraksi atas dan fraksi bawah terdapat pada T1 (75,00% dan 71,33%), diikuti oleh T2 (69,92% dan 68,42%) dan T3 (66,92% dan 65,58%). Hasil penelitian dapat disimpulkan bahwa waktu inkubasi 45 menit merupakan waktu inkubasi yang optimum pada proses sexing sperma berbasis glutathione, sehingga motilitas dan MPU chilled semen sexing domba lokal dapat dipertahankan. Kata Kunci: waktu inkubasi, sexing sperma, glutathione, semen domba AbstractThe aim of this study was to examine the effect of incubation time on sexing process based on glutathione to the motility and Intact Plasma Membrane (IPM) of chilled ram semen. The separation of X and Y sperm conducted by using BSA method. This research was experimental research using completely randomized design (CRD) with three treatment namely, 45 minutes (T1), 60 minutes (T2) and 75 minutes (T3). Data were analyzed using Anova followed by Duncan's multiple range test. Material used in this research was the fresh semen from ram with sperm motility ≥ 70% and 5 mM of glutathione. The result of this research showed that the highest percentage of motility in upper and bottom fraction belong to T1 (69.73% and 68.82%), followed by T2(66.40% and 64.55%)and T3(57.90% and 57.65%). In addition, the result of this research that the highest percentage of IPM in upper and bottom fraction belong to T (75.00% and 71.33%), followed by T2(69.92% and68.42%) and T3 (66.92% dan 65.58%). It can be concluded that the incubation time of 45 minute is the optimum time on sexing process based on glutathione so that the motility and IPM of chilled ram semen can be maintained.

The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty

“…The five groups were then incubated at 37℃ for 1 hour. 100 μL of the sample from 5 groups mixed with 1 mL of HOS solution, then incubated for 30 minutes at 37 0C (Yusrina et al, 2018). Place ten μL of the sample into a slide, and the sperm membrane integrity with HOS was examined by microscope with a magnification of 400 x.…”