Penicillin-Binding Proteins (PBPs) and Bacterial Cell Wall Elongation Complexes

Miyachiro, Mayara M.; Contreras‐Martel, Carlos; Dessen, Andréa

doi:10.1007/978-3-030-28151-9_8

Cited by 35 publications

(32 citation statements)

References 80 publications

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“…Although the upregulated expression of the above-mentioned cell-wall-related genes may be the molecular mechanism underlying the thickened cell wall of this hVISA strain, we observed that penicillin binding proteins (PBPs) were also upregulated, and this result was differed from the findings of previous reports (Sieradzki and Tomasz 2003;Navratna et al 2010). PBPs are carboxypeptidase and transpeptidase and are mainly involved in the synthesis of the murein of the cell wall and the secondary cross-linking of peptidoglycan, which play an important role in the growth and reproduction of bacteria and the maintenance of normal morphology (Miyachiro et al 2019). A previous study suggested that after the PBP4 gene of S. aureus was knocked out, the cross-linking of peptidoglycan decreased significantly, VAN binding…”

Section: Rt-qpcr Results Of Cell-wall-related Genescontrasting

confidence: 86%

“…PBPs are carboxypeptidase and transpeptidase and are mainly involved in the synthesis of the murein of the cell wall and the secondary cross‐linking of peptidoglycan, which play an important role in the growth and reproduction of bacteria and the maintenance of normal morphology (Miyachiro et al . 2019). A previous study suggested that after the PBP4 gene of S. aureus was knocked out, the cross‐linking of peptidoglycan decreased significantly, VAN binding sites decreased and resistance to VAN increased (Navratna et al .…”

Section: Resultsmentioning

confidence: 99%

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Cell wall thickness and the molecular mechanism of heterogeneous vancomycin‐intermediateStaphylococcus aureus

Cui

Zhang

et al. 2021

Lett Appl Microbiol

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Significance and impact of the study: We isolated a heterogeneous vancomycin (VAN)-intermediate Staphylococcus aureus (hVISA) strain, and we demonstrated that the cell wall of the hVISA strain was significantly thicker than that of ordinary MRSA strains. The upregulated expression of glmS, vraR/S, sgtB, murZ and PBP4 genes related to cell wall synthesis might be the molecular mechanism underlying the cell wall thickening of the hVISA strain and might be related to its reduced sensitivity to VAN. These results could be used as a supplement to other hVISA-related studies in the world and might be beneficial to prevent and control the emergence of more hVISA.

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Section: Rt-qpcr Results Of Cell-wall-related Genescontrasting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Cell wall thickness and the molecular mechanism of heterogeneous vancomycin‐intermediateStaphylococcus aureus

Cui

Zhang

et al. 2021

Lett Appl Microbiol

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“…While the cell wall helps protect cytoplasmic antimicrobial targets, it also ended up being the target for the first natural antibiotic, penicillin, which prevents the complete formation of this barrier by inhibiting peptide cross-linking to occur [ 79 ]. With a faulty protective structure, the cell becomes vulnerable to its internal environment and the external environment, leading to cell death [ 80 ].…”

Section: Protection Of Antimicrobial Targetsmentioning

confidence: 99%

Bacterial Resistance to Antimicrobial Agents

et al. 2021

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Bacterial pathogens as causative agents of infection constitute an alarming concern in the public health sector. In particular, bacteria with resistance to multiple antimicrobial agents can confound chemotherapeutic efficacy towards infectious diseases. Multidrug-resistant bacteria harbor various molecular and cellular mechanisms for antimicrobial resistance. These antimicrobial resistance mechanisms include active antimicrobial efflux, reduced drug entry into cells of pathogens, enzymatic metabolism of antimicrobial agents to inactive products, biofilm formation, altered drug targets, and protection of antimicrobial targets. These microbial systems represent suitable focuses for investigation to establish the means for their circumvention and to reestablish therapeutic effectiveness. This review briefly summarizes the various antimicrobial resistance mechanisms that are harbored within infectious bacteria.

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“…The crystal structures of several high and low molecular weight PBPs from various organisms have been determined in the last few years (for an overview see Mattei et al, 2010;Miyachiro et al, 2019;Sauvage and Terrak, 2016). TP domains show high similarity, with a central, mixed, β-sheet surrounded by αhelices.…”

Section: Structure Of Pbpsmentioning

confidence: 99%