Peptide Growth Factors Modulate Prostaglandin E and F Production by Goldfish Ovarian Follicles

MacDougall, Thomas M.; Kraak, Glen Van Der

doi:10.1006/gcen.1997.7047

Cited by 13 publications

(2 citation statements)

References 31 publications

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“…In the few existing works on Egfr signaling within fish species, only recombinant heterologous ligands from mammals have been used to activate fish Egfrs [9,17,18]. In these studies, mammalian Egf, but also Tgfa, Hbegf and Btc were described to cross-react with the fish receptor.…”

Section: Resultsmentioning

confidence: 99%

“…Importantly, all previous studies in fish (zebrafish, goldfish and trout) that included the application of Egf receptor ligands were performed using recombinant mammalian proteins [9,17,18]. This implies that mammalian Egfr and Egfr ligands share high structural similarity and functional homology with their fish counterparts and that mammalian ligands consequently have the ability to interact with the fish Egfr binding domain to trigger its dimerization and activation.…”

Section: Introductionmentioning

confidence: 99%

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Lineage-specific co-evolution of the Egf receptor/ligand signaling system

et al. 2010

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BackgroundThe epidermal growth factor receptor (Egfr) with its numerous ligands has fundamental roles in development, cell differentiation and physiology. Dysfunction of the receptor-ligand system contributes to many human malignancies. Consistent with such various tasks, the Egfr gene family has expanded during vertebrate evolution as a consequence of several rounds of whole genome duplication. Of particular interest is the effect of the fish-specific whole genome duplication (FSGD) on the ligand-receptor system, as it has supplied this largest group of vertebrates with additional opportunities for sub- and/or neofunctionalization in this signaling system.ResultsWe identified the predicted components of the Egf receptor-ligand signaling system in teleost fishes (medaka, platyfish, stickleback, pufferfishes and zebrafish). We found two duplicated egfr genes, egfra and egfrb, in all available teleost genomes. Surprisingly only one copy for each of the seven Egfr ligands could be identified in most fishes, with zebrafish hbegf being the only exception. Special focus was put on medaka, for which we more closely investigated all Egf receptors and Egfr ligands. The different expression patterns of egfra, egfrb and their ligands in medaka tissues and embryo stages suggest differences in role and function. Preferential co-expression of different subsets of Egfr ligands corroborates the possible subfunctionalization and specialization of the two receptors in adult tissues. Bioinformatic analyses of the ligand-receptor interface between Egfr and its ligands show a very weak evolutionary conservation within this region. Using in vitro analyses of medaka Egfra, we could show that this receptor is only activated by medaka ligands, but not by human EGF. Altogether, our data suggest a lineage-specific Egfr/Egfr ligand co-evolution.ConclusionsOur data indicate that medaka Egfr signaling occurs via its two copies, Egfra and Egfrb, each of them being preferentially coexpressed with different subsets of Egfr ligands. This fish-specific occurrence of Egf receptor specialization offers unique opportunities to study the functions of different Egf receptor-ligand combinations and their biological outputs in vertebrates. Furthermore, our results strongly support the use of homologous ligands in future studies, as sufficient cross-specificity is very unlikely for this ligand/receptor system.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lineage-specific co-evolution of the Egf receptor/ligand signaling system

et al. 2010

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Gonadotropins and their paracrine signaling network in the zebrafish ovary

2005

Fish Physiol Biochem

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Pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), play fundamental roles in vertebrate ovarian development and function. However, there has been an increasing body of evidence that the actions of FSH and LH are mediated or modulated by a variety of locally produced peptide or protein factors, which form an intimate regulatory network within and between the ovarian follicles. In the past few years, a variety of growth factors have been identified and characterized in the zebrafish ovary including activin and epidermal growth factor (EGF), which are important components of the intraovarian communication network. To understand how this local network interacts with the gonadotropins from the pituitary, we have recently cloned and characterized all the subunits of zebrafish FSH and LH from the pituitary as well as their receptors (FSHR and LHR) from the ovary. Using the Chinese hamster ovary (CHO) cells as the bioreactor, we have produced recombinant zebrafish FSH and LH with biological activities. With the recombinant hormones available, the functions of zebrafish FSH and LH in the ovary and their interactions with the local factors will be an important issue to address in the future. This review briefly summarizes some recent work from our laboratory and others on both gonadotropins and their potential intraovarian signaling factors in the zebrafish.

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Endocrine System

Janz

2000

The Laboratory Fish

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Peptide Growth Factors Modulate Prostaglandin E and F Production by Goldfish Ovarian Follicles

Cited by 13 publications

References 31 publications

Lineage-specific co-evolution of the Egf receptor/ligand signaling system

Lineage-specific co-evolution of the Egf receptor/ligand signaling system

Gonadotropins and their paracrine signaling network in the zebrafish ovary

Endocrine System

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