Perbanyakan Mikro Colocasia esculenta (L.) Schott var. Antiquorum Melalui Penggunaan IAA

Louw, Asnad E.; Kesaulya, Henry; Lawalata, Imelda Jeanette

doi:10.30598/jbdp.2018.14.1.28

Cited by 4 publications

(7 citation statements)

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“…This is presumably because the more the concentration is added, the number of Cavendish banana plantlet roots decreases. Louw et al (2018) explained that explants already contained sufficient endogenous auxin content for root growth, if high exogenous auxin was given it would also inhibit root growth. Widiastoety (2014) also added that low light intensity can stimulate endogenous growth regulators to work more actively to carry out the process of root growth and development.…”

Section: Number Of Rootsmentioning

confidence: 99%

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

Sholikhah

Makhziah²,

Widiwurjani³

2022

JTEP-L

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In vitro propagation of Cavendish banana is an alternative to produce healthy, uniform and sufficient plant materials of high qualities. Appropriate culture media formulation is needed for shoot growth and rooting stage. This experiment aimed to study effects of IAA and organic supplements on in vitro shoot growth and rooting. Explants were subjected to MS medium with IAA and various organic supplements, in a factorial arrangement of a completely randomized design. The first factor was IAA concentrations (0; 0.5; 1 and 1.5 mg.L-1), and the second factor was types of organic supplement (control, coconut water, potato extract and banana extract). At 8 weeks after planting, shoot growth and root performance of plantlets were recorded. The results showed that addition of IAA did not affect shoot height. Addition of potato extract and banana homogenate resulted in the highest plantlets. Medium with IAA at 1 mg.L-1 resulted in the highest number of roots. The highest number of shoots was produced on media with IAA 1 mg.L-1 + coconut water and IAA 1.5 mg.L-1 + potato extract. The highest number of roots was produced in medium with potato extract. The longest roots were produced in media with 0.5 mg.L-1 IAA + banana homogenate. Keywords: Banana extract, Cavendish, Coconut water, IAA, Potato extract

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Section: Number Of Rootsmentioning

confidence: 99%

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

Sholikhah

Makhziah²,

Widiwurjani³

2022

JTEP-L

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“…Three shoots were produced by the B2 (BAP 2 mg/l), which yielded the least amount. Louw et al (2018), BAP is a kind of cytokinin with strong activity in cell division and bud formation. In the interaction between cytokinins and auxins, the action of cytokinins cannot be separated from that of auxins, as the effects of blocking or stimulating cell division depend on the phytohormones interacting.…”

Section: Effect On the Number Of Shootsmentioning

confidence: 99%

EFFECT OF NAA AND BAP APPLICATION ON THE GROWTH RESPONSES OF Mentha × piperita L.

Wijaya¹,

Safrina²,

Herera

et al. 2022

J. Tumbuhan Obat Indonesia

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Mentha × piperita L., also known as peppermint, is a plant with various uses, including medicine, cosmetics, and food. Numerous industries have a high need for peppermint products, but Indonesia is currently unable to meet this demand and should continue to import peppermint. One effort can be made to improve cultivation procedures, and tissue culture becomes one alternative. This research uses shoots as explants with Murashige & Skoog's basic media and growth regulators BAP and NAA. The research was conducted in two phases: six-week initial planting and seven-week subculture. The treatment of BAP 4 mg/L + NAA 0.5 mg/L provides better performance for the number of leaves, and BAP 3 mg/L produced the best response regarding the number of shoots. Furthermore, BAP 1 mg/L + NAA 1 mg/L produced the best response to shoot height and number of leaves, and BAP 3 mg/L + NAA 0.5 mg/L generated the best response to root length. Based on the research, BAP 3 mg/L is the optimal treatment.

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“…Faktor zat pengatur tumbuh yang tidak konstan dalam eksplan sehingga menghasilkan respon yang tidak sesuai (Santoso & Nursandi, 2004). Louw et al (2018), menambahkan bahwa konsentrasi IAA yang diberikan semakin tinggi akan menghambat pertumbuhan daun.Berdasarkan penelitian yang dilakukan jumlah daun talas satoimo perlakuan B1I2 (BAP 5 µM dan IAA 2 µM) menghasilkan jumlah daun terbanyak (Gambar 5). Akar merupakan organ yang beperan penting dan harus dimiliki eksplan.…”

Section: Gambar 3 Kemunculan Tunas Perlakuan B1i2unclassified

“…Akar berfungsi untuk menyerap unsur hara dari media tanam untuk perumbuhan dan perkembangan eksplan. Perakaran harus memiliki kualitas yang baik untuk proses aklimatisasi berjalan dengan baik (Louw et al, 2018). Berdasarkan data rataan jumlah akar yang diperoleh setelah 8 mst (Gambar 6) menunjukkan bahwa perlakuan B1I3 (BAP 5 µM dan IAA 3 µM) menghasilkan rataan jumlah akar paling tinggi yaitu 14 akar.…”

Section: Gambar 3 Kemunculan Tunas Perlakuan B1i2unclassified

“…Hasil analisis ragam pengaruh interaksi berpengaruh nyata terhadap BAP dan IAA yang menunjukan bahwa pemberian konsentrasi IAA dan BAP yang tinggi akan menghambat pertumbuhan akar. Hal sesuai dengan penelitian Louw et al (2018), eksplan sudah memiliki kandungan auksin endogen yang cukup untuk pertumbuhan akar, apabila diberikan auksin eksogen yang tinggi pula akan menghambat pertumbuhan akar maupun akar menjadi pendek. Hal ini didukung oleh Marlin (2005), eksplan dapat tumbuh dan berkembang menjadi tanaman baru tanpa pemberian zat pengatur tumbuh seperti auksin dan sitokinin akan tetap tumbuh dan mengalami elongasi.…”

Section: Gambar 3 Kemunculan Tunas Perlakuan B1i2unclassified

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PENGARUH INTERAKSI ANTARA BAP DAN IAA TERHADAP MULTIPLIKASI TUNAS TALAS SATOIMO (Colocasia esculenta (L.) Schott var. antiquorum) SECARA IN VITRO

Ilham¹,

Sugiyono²,

Prayoga³

2019

bioe

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Talas satoimo (Colocasia esculenta (L.) Schott var. antiquorum) adalah jenis talas yang memiliki ukuran umbi kecil (small corm taro) dan disebut sebagai talas Jepang atau satoimo. Metode perbanyakan satoimo secara konvensional memerlukan waktu relatif lama, oleh karenanya teknik perbanyakan tunas satoimo secara in vitro menjadi alternatif untuk memenuhi kebutuhan bibit satoimo yang terus meningkat. Penelitian ini dilakukan dengan tujuan untuk mempelajari pengaruh interaksi antara BAP dan IAA pada multiplikasi tunas satoimo dan menentukan konsentrasi BAP dan IAA paling baik untuk multiplikasi tunas satoimo. Penelitian dilaksanakan secara eksperimental dengan Rancangan Acak Lengkap (RAL) dengan pola perlakuan faktorial. Faktor pertama adalah konsentrasi BAP (B) dengan 4 taraf yaitu B1 : 5 µM, B2 : 7,5 µM, B3 : 10 µM dan B4 : 12,5 µM. Faktor kedua adalah konsentrasi IAA (I) dengan 4 taraf yaitu I1 : 1 µM, I2 : 2 µM, I3 : 3 µM dan I4 : 4 µM. Kombinasi kedua faktor ini menghasilkan 16 perlakuan. Setiap kombinasi perlakuan diulang sebanyak 3 kali, sehingga terdapat 48 unit percobaan. Variabel yang diamati adalah pertumbuhan tunas talas, dengan parameter yang diukur meliputi jumlah tunas, jumlah daun, dan jumlah akar. Data hasil penelitian yang diperoleh dianalisis dengan Analisis Ragam dengan tingkat kepercayaan 95%, dilanjutkan dengan uji Beda Nyata Terkecil (BNT) pada tingkat kesalahan 5%. Hasil penelitian menunjukkan bahwa interaksi antara BAP dan IAA memacu pembentukan tunas dan akar pada multiplikasi tunas satoimo dalam kultur in vitro. BAP dengan konsentrasi 5 µM dan IAA konsentrasi 2 µM merupakan konsentrasi terbaik untuk memacu multiplikasi talas satoimo dalam kultur in vitro.

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Perbanyakan Mikro Colocasia esculenta (L.) Schott var. Antiquorum Melalui Penggunaan IAA

Cited by 4 publications

References 1 publication

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

EFFECT OF NAA AND BAP APPLICATION ON THE GROWTH RESPONSES OF Mentha × piperita L.

PENGARUH INTERAKSI ANTARA BAP DAN IAA TERHADAP MULTIPLIKASI TUNAS TALAS SATOIMO (Colocasia esculenta (L.) Schott var. antiquorum) SECARA IN VITRO

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