A growing body of research affirms the significance of physician-nurse collaboration for the delivery of aquality patient care and better health outcomes. In this descriptive comparative study, the difference in attitudes toward collaboration between physicians and nurses was compared. Data were obtained through distribution of survey questionnaire which is an adaptation of the Jefferson Scale of Attitudes toward Physician-Nurse Collaboration (JSATPNC) to the practicing physicians and registered nurses at The Doctors' Clinic and Hospital Incorporated (TDCHI), Philippines. The mean scores on attitudes toward collaboration for physicians (n = 48) was 48.9 (± SD 6.16) and 51.2 (± SD 5.46) for nurses group (n=94). Mean scores were consistently higher for nurses as compared with physicians. Analysis demonstrated that this difference is statistically significant (t = 2.272 with P value of 0.0246< .05). Further analysis showed significant relationship between the physicians' number of years in practice with their attitudes towards collaboration but not with gender, age, and tribe. Results showed significant relationship between nurses' age and their attitude towards collaboration but failed to demonstrate significant relationship based on gender, tribe, and years in practice. Results were analyzed and used as bases to identify target areas for improving physician-nurse collaboration at TDCHI. The recommended plans and strategies were included in the revised TDCHI Training Manual.