Performance characteristics of a rapid immunochromatographic assay for detection of pandemic influenza A (H1N1) virus in children

Keitel, Kristina; Wagner, Noémie; Lacroix, Laurence; Manzano, Sergio; Gervaix, Alain

doi:10.1007/s00431-010-1326-0

Cited by 14 publications

(9 citation statements)

References 17 publications

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“…The broad viral sensitivity of this assay may be a distinct advantage over pathogen-based antigen or PCR testing should a new influenza virus, or potentially other viral type, begin to circulate in the community. Such an advantage might have been demonstrated in the 2009 H1N1 pandemic when traditional antigen-based influenza A testing failed to detect most of the adults infected with the new H1N1 influenza virus (7), delaying appropriate treatment and identification of the causative agent. This assay may also have proved advantageous in the more recent H3N2 influenza virus outbreak in 2012 (17) or in other new influenza virus outbreaks such as the recent avian influenza A virus subtype H7N9 outbreak (18).…”

Section: Discussionmentioning

confidence: 99%

A Host-Based RT-PCR Gene Expression Signature to Identify Acute Respiratory Viral Infection

et al. 2013

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Improved ways to diagnose acute respiratory viral infections could decrease inappropriate antibacterial use and serve as a vital triage mechanism in the event of a potential viral pandemic. Measurement of the host response to infection is an alternative to pathogen-based diagnostic testing and may improve diagnostic accuracy. We have developed a host-based assay with a reverse transcription polymerase chain reaction (RT-PCR) TaqMan low-density array (TLDA) platform for classifying respiratory viral infection. We developed the assay using two cohorts experimentally infected with influenza A H3N2/Wisconsin or influenza A H1N1/Brisbane, and validated the assay in a sample of adults presenting to the emergency department with fever (n = 102) and in healthy volunteers (n = 41). Peripheral blood RNA samples were obtained from individuals who underwent experimental viral challenge or who presented to the emergency department and had microbiologically proven viral respiratory infection or systemic bacterial infection. The selected gene set on the RT-PCR TLDA assay classified participants with experimentally induced influenza H3N2 and H1N1 infection with 100 and 87% accuracy, respectively. We validated this host gene expression signature in a cohort of 102 individuals arriving at the emergency department. The sensitivity of the RT-PCR test was 89% [95% confidence interval (CI), 72 to 98%], and the specificity was 94% (95% CI, 86 to 99%). These results show that RT-PCR–based detection of a host gene expression signature can classify individuals with respiratory viral infection and sets the stage for prospective evaluation of this diagnostic approach in a clinical setting.

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Section: Discussionmentioning

confidence: 99%

A Host-Based RT-PCR Gene Expression Signature to Identify Acute Respiratory Viral Infection

et al. 2013

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“…We predict that the condition of suspension buffer, membrane, and antibodies were the reason why conventional IC has dramatically improved since 2006, when a report was published by Keitel et al . [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Fluorescent Immunochromatography for Rapid and Sensitive Typing of Seasonal Influenza Viruses

Sakurai

Takayama²,

Nomura

et al. 2015

PLoS ONE

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Lateral flow tests also known as Immunochromatography (IC) is an antigen-detection method conducted on a nitrocellulose membrane that can be completed in less than 20 min. IC has been used as an important rapid test for clinical diagnosis and surveillance of influenza viruses, but the IC sensitivity is relatively low (approximately 60%) and the limit of detection (LOD) is as low as 10³ pfu per reaction. Recently, we reported an improved IC assay using antibodies conjugated with fluorescent beads (fluorescent immunochromatography; FLIC) for subtyping H5 influenza viruses (FLIC-H5). Although the FLIC strip must be scanned using a fluorescent reader, the sensitivity (LOD) is significantly improved over that of conventional IC methods. In addition, the antibodies which are specific against the subtypes of influenza viruses cannot be available for the detection of other subtypes when the major antigenicity will be changed. In this study, we established the use of FLIC to type seasonal influenza A and B viruses (FLIC-AB). This method has improved sensitivity to 100-fold higher than that of conventional IC methods when we used several strains of influenza viruses. In addition, FLIC-AB demonstrated the ability to detect influenza type A and influenza type B viruses from clinical samples with high sensitivity and specificity (Type A: sensitivity 98.7% (74/75), specificity 100% (54/54), Type B: sensitivity 100% (90/90), specificity 98.2% (54/55) in nasal swab samples) in comparison to the results of qRT-PCR. And furthermore, FLIC-AB performs better in the detection of early stage infection (under 13h) than other conventional IC methods. Our results provide new strategies to prevent the early-stage transmission of influenza viruses in humans during both seasonal outbreaks and pandemics.

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“…IC is rapid and easy to use, but has a relatively low sensitivity [40,41]. The specificity is >90%, whereas the sensitivity is only ~60%.…”

Section: Immunochromatographymentioning

confidence: 99%

“…The specificity is >90%, whereas the sensitivity is only ~60%. Thus, the technique is not suitable for the diagnosis of IAVs in the early stages of infection [41]. For detection of the H5 strain, the sensitivity of IC using H5-specific antibody was reported to be 10 4.5 –10 6 50 % egg infectious dose (EID 50 )/mL [42].…”

Section: Immunochromatographymentioning

confidence: 99%

Updated Values for Molecular Diagnosis for Highly Pathogenic Avian Influenza Virus

Sakurai¹,

Shibasaki²

2012

Viruses

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Highly pathogenic avian influenza (HPAI) viruses of the H5N1 strain pose a pandemic threat. H5N1 strain virus is extremely lethal and contagious for poultry. Even though mortality is 59% in infected humans, these viruses do not spread efficiently between humans. In 1997, an outbreak of H5N1 strain with human cases occurred in Hong Kong. This event highlighted the need for rapid identification and subtyping of influenza A viruses (IAV), not only to facilitate surveillance of the pandemic potential of avian IAV, but also to improve the control and treatment of infected patients. Molecular diagnosis has played a key role in the detection and typing of IAV in recent years, spurred by rapid advances in technologies for detection and characterization of viral RNAs and proteins. Such technologies, which include immunochromatography, quantitative real-time PCR, super high-speed real-time PCR, and isothermal DNA amplification, are expected to contribute to faster and easier diagnosis and typing of IAV.

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Performance characteristics of a rapid immunochromatographic assay for detection of pandemic influenza A (H1N1) virus in children

Cited by 14 publications

References 17 publications

A Host-Based RT-PCR Gene Expression Signature to Identify Acute Respiratory Viral Infection

A Host-Based RT-PCR Gene Expression Signature to Identify Acute Respiratory Viral Infection

Fluorescent Immunochromatography for Rapid and Sensitive Typing of Seasonal Influenza Viruses

Updated Values for Molecular Diagnosis for Highly Pathogenic Avian Influenza Virus

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