Performance evaluation of Alfred60AST rapid susceptibility testing directly from positive blood cultures in the routine laboratory workflow

Mantzana, Paraskevi; Netsika, Fotini; Arhonti, Maria; Meletis, Georgios; Kandilioti, Eleni; Kiriakopoulou, M; Kagkalou, Georgia; Vasilaki, Olga; Tychala, Areti; Protonotariou, Efthimia; Skoura, Lemonia

doi:10.1007/s10096-021-04191-9

Cited by 9 publications

(6 citation statements)

References 21 publications

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“…Results of piperacillin-tazobactam AST were improved after the reagent was revised (Boland et al, 2019;Mantzana et al, 2021) as well as in our evaluation where we also found a major decrease in VME and ME. We should note that Mantzana et al ( 2021) evaluated Alfred 60/AST during 2 periods (May 2018 to July 2018 and March 2019 to February 2020) but they didn't describe a higher piperacillin-tazobactam discrepancy rate in the first period compared to the second period, as it was discussed by Boland et al (2019).…”

Section: First Objective: Assessment Of the Analytical Performances O...supporting

confidence: 76%

“…However, one out of 3 MRSA strain was not detected by Alfred 60/AST (VME rate of 33.3%). A recent study (Mantzana et al, 2021) also highlighted a high VME rate of 15.3% for the cefoxitin evaluation in S. aureus strains. This is maybe due to a short incubation method (3 h), Alifax® is working on changing the analysis length to 5 h. Anyway, other rapid diagnostic assays could represent an alternative for quick MRSA detection.…”

Section: First Objective: Assessment Of the Analytical Performances O...mentioning

confidence: 97%

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Antimicrobial susceptibility testing determined by Alfred 60/AST (Alifax®) in a multi-sites lab: performance's evaluation and optimization of workflow

Cupaiolo

Cherkaoui

Serrano

et al. 2022

Journal of Microbiological Methods

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Section: First Objective: Assessment Of the Analytical Performances O...supporting

confidence: 76%

Section: First Objective: Assessment Of the Analytical Performances O...mentioning

confidence: 97%

Antimicrobial susceptibility testing determined by Alfred 60/AST (Alifax®) in a multi-sites lab: performance's evaluation and optimization of workflow

Cupaiolo

Cherkaoui

Serrano

et al. 2022

Journal of Microbiological Methods

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“…in 2019, 94.1 % Mantzana et al . in 2021) and the agreement values and errors were within the range of acceptability [15–18, 22]. Only co-trimoxazole and piperacillin/tazobactam registered a low agreement with reference methods as already observed by Giordano et al (e.g .…”

Section: Discussionmentioning

confidence: 55%

“…Other studies have evaluated the accuracy and TAT of the combination of MALDI-TOF MS and ALFRED60AST, but, to our knowledge, no previous studies have investigated their potential impact on clinical management of septic patients in a real-life setting [15][16][17][18][28][29][30]. Available data on the impact of other rapid combined methods are few and difficult to compare.…”

Section: Discussionmentioning

confidence: 99%

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Light Scattering Technology and MALDI-TOF MS in the microbiological fast-track of bloodstream infections: potential impact on antimicrobial treatment choices in a real-life setting

Curtoni

Ghibaudo

Veglio

et al. 2023

Journal of Medical Microbiology

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Introduction. Rapid identification (ID) and antimicrobial susceptibility testing (AST) of bloodstream infections (BSI) pathogens are fundamental to switch from empirical to targeted antibiotic therapy improving patients outcome and reducing antimicrobial resistance spreading. Hypothesis. The adoption of a rapid microbiological protocol (RP) based on Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) and Light Scattering Technology (LST) for rapid diagnosis of BSI could positively impact on patients' antimicrobial management. Aim. The study aim was to evaluate a RP for BSI microbiological diagnosis in terms of accuracy, turnaround time (TAT) and potential therapeutic impact. Methodology. A prospective observational study was conducted: monomicrobial bacterial blood cultures of septic patients were analysed in parallel by RP and standard protocol (SP). In RP the combination of MALDI-TOF MS and LST was used for rapid ID and AST assessments, respectively. To determine the potential impact of RP on antimicrobial therapy management, clinicians were interviewed on therapeutic decisions based on RP and SP results. RP accuracy, TAT and impact were evaluated in comparison to SP results. Results. A total of 97 patients were enrolled. ID and AST concordance between RP and SP were 96.9 and 94.7 %, respectively. RP technical and real-life TAT were lower than SP (6.4 h vs. 18.4 h; 9.5 vs. 27.1 h). The agreement between RP- and SP-based therapeutic decisions was 90.7 (90 % CI 84.4–95.1). RP results could produce 24/97 correct antibiotic changes with 18/97 possible de-escalations and 25/97 prompt applications of infection control precautions. Conclusion. With the application of RP in BSI management, about one-fourth of patients may safely benefit from early targeted antibiotic therapy and infection control policies with one working day in advance in comparison to conventional methods. This protocol is feasible for clinical use in microbiology laboratories and potentially helpful for Antimicrobial Stewardship.

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Laboratory Detection of Bacteremia and Fungemia

T. Harrington,

Jean,

Hilt

2023

ClinMicroNow

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Clinical microbiology laboratories play an essential role in establishing an etiologic diagnosis for sepsis and bloodstream infection. Diagnosis requires multiple steps, including one or more blood cultures drawn from different sites, recovery and identification of microorganisms detected in blood culture, antimicrobial susceptibility testing of clinically significant organisms, and interpretation of results in conjunction with other clinical, radiographic, and laboratory findings. This chapter provides a summary of the clinical importance of bacteremia and fungemia, the scientific and medical principles underlying current diagnostic methods, and diagnostic approaches. Determining the clinical significance can be difficult for coagulase‐negative staphylococci, as the identity of this organism group is of little or no help. Preparation for blood culture collection and transport to the laboratory includes a number of stepwise processes. Matrix‐assisted laser desorption ionization–time‐of‐flight mass spectrometry is an extensively studied approach to rapid microbial identification of blood culture isolates.

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Performance evaluation of Alfred60AST rapid susceptibility testing directly from positive blood cultures in the routine laboratory workflow

Cited by 9 publications

References 21 publications

Antimicrobial susceptibility testing determined by Alfred 60/AST (Alifax®) in a multi-sites lab: performance's evaluation and optimization of workflow

Antimicrobial susceptibility testing determined by Alfred 60/AST (Alifax®) in a multi-sites lab: performance's evaluation and optimization of workflow

Light Scattering Technology and MALDI-TOF MS in the microbiological fast-track of bloodstream infections: potential impact on antimicrobial treatment choices in a real-life setting

Laboratory Detection of Bacteremia and Fungemia

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