Performance Evaluation of Nine Hormone Assays on the Immulite 2000®Immunoassay System

Tello, Francisca Llinares; Hernández, Dolores Mirete

doi:10.1515/cclm.2000.154

Cited by 18 publications

(7 citation statements)

References 4 publications

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“…This contradiction highlights the need for careful evaluation of each assay used for the diagnosis of CPP. The Immulite 2000 LH assay showed good correlation to the Magia LH assay (10).…”

Section: Discussionmentioning

confidence: 89%

The utility of basal serum LH in prediction of central precocious puberty in girls

Pasternak

Friger

Loewenthal

et al. 2012

European Journal of Endocrinology

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Objective: The mainstay of distinction between prepubertal girls and girls who are suspected of having central precocious puberty (CPP) is based on gonadotropin measurements after a GnRH stimulation test to evaluate hypothalamic-pituitary-gonadal axis maturity. The objective of this study was to determine whether a single basal gonadotropin measurement carries a useful predictive value in verifying or refuting the diagnosis of CPP. Design and methods: Basal serum LH and FSH were measured by a chemiluminescent immunometric assay in a cohort of girls who had been evaluated for CPP before and after GnRH stimulation test. Peak LH levels higher than 5 IU/l were considered a pubertal response. Results: Eighty girls with suspected breast development before 8 years of age were enrolled to the study, out of whom 42 had CPP. Low basal serum LH (%0.1 IU/l) was sufficient to rule out the diagnosis of CPP in 94.7% of the 38 prepubertal girls; the sensitivity of basal LH levels for this purpose was only 64%. The basal FSH and the basal LH to FSH ratio achieved less efficient predictive value with 76 and 71% sensitivity and 73 and 86% specificity respectively. Conclusion: A single basal LH measurement may be adequate to confirm but not to refute the presence of CPP in most of the girls who are evaluated for early pubertal signs.

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“…This contradiction highlights the need for careful evaluation of each assay used for the diagnosis of CPP. The Immulite 2000 LH assay showed good correlation to the Magia LH assay (10).…”

Section: Discussionmentioning

confidence: 89%

The utility of basal serum LH in prediction of central precocious puberty in girls

Pasternak

Friger

Loewenthal

et al. 2012

European Journal of Endocrinology

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“…No significant differences were observed for the cortisol and cortisone concentrations and for the cortisone/cortisol ratio between the genders. Previously, cortisol concentrations in plasma for adults were reported to be 60 -230 Ag/ l by an IA-based method [16]. Lower reference intervals for urinary free cortisol were reported in adult females by LC-MS/MS based methods compared with IA methods [17,20].…”

Section: Discussionmentioning

confidence: 99%

“…Historically, immunoassay (IA)-based methods were widely used for cortisol analysis [16]. These methods may be susceptible to interference from cortisone and other steroid metabolites.…”

Section: Introductionmentioning

confidence: 99%

Cortisol and cortisone analysis in serum and plasma by atmospheric pressure photoionization tandem mass spectrometry

Kushnir

Neilson

Roberts

et al. 2004

Clinical Biochemistry

112

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“…Progesterone and estradiol levels were measured using the Immulite 2000 immunoassay analyzer (Diagnostic Products Corporation, Los Angeles, CA) that employs electrochemical luminescence for detection. 42 Lower limits of detection for estradiol and progesterone were 10 pg/mL and 0.1 ng/mL, respectively.…”

Section: Estrogen and Progesterone Hormonal Levelsmentioning

confidence: 93%

The Effect of Progesterone Levels and Pregnancy on HIV-1 Coreceptor Expression

et al. 2009

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The upregulation of HIV-1 co-receptor expression during certain clinical settings may explain the predisposition of individuals to enhanced HIV-1 acquisition. We sought to determine the effect of estrogen and progesterone on the HIV-1 coreceptors, CCR5 and CXCR4. Co-receptor expression on CD3- and CD14-positive cells obtained systemically and locally (genital tissue in women) was determined in men, pre- and post-menopausal women, pregnant women in each trimester and in labor. CCR5 on both CD3- and CD14-positive cells was highest in pregnant women, and increased as gestation advanced ( P < .01 and P < .001, respectively). Progesterone levels were significantly associated with CCR5 expression on PBMCs ( P < .03 for CD3-positive, and P < .002 for CD14-positive cells) and from cells isolated from tissue ( P < .001).CCR5 mRNA expression correlated with the cell surface marker expression from blood and tissue. These findings suggest that pregnancy and other high progesterone states may predispose women to HIV-1 acquisition.

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Performance Evaluation of Nine Hormone Assays on the Immulite 2000®Immunoassay System

Cited by 18 publications

References 4 publications

The utility of basal serum LH in prediction of central precocious puberty in girls

The utility of basal serum LH in prediction of central precocious puberty in girls

Cortisol and cortisone analysis in serum and plasma by atmospheric pressure photoionization tandem mass spectrometry

The Effect of Progesterone Levels and Pregnancy on HIV-1 Coreceptor Expression

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