Performance of a sulfidogenic bioreactor inoculated with indigenous acidic communities for treating an extremely acidic mine water

González, Daniella; Liu, Yun; Gómez, Denys Kristalia Villa; Southam, Gordon; Hedrich, Sabrina; Galleguillos, Pedro A.; Colipai, Camila; Nancucheo, Iván

doi:10.1016/j.mineng.2018.11.011

Cited by 28 publications

(9 citation statements)

References 31 publications

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“…Next, individual colonies from each pure culture were resuspended in a basal medium of salts to extract DNA (typically 1 µL) for the amplification of the 16S rRNA gene [34] using the primer pairs 27F [35] and 1492R [36]. The PCR products were sequenced (Macrogen, Inc., Seoul, South Korea) and aligned using the BLASTN online software (NCBI) and compared with the GenBank database [37].…”

Section: Microbiological and Molecular Analysismentioning

confidence: 99%

Evaluation of Dispersed Alkaline Substrate and Diffusive Exchange System Technologies for the Passive Treatment of Copper Mining Acid Drainage

Schwarz

Nancucheo

Gaete

et al. 2020

Water

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The study evaluates the performance of the novel ADES (alkaline diffusive exchange System), SDES (sulfidogenic diffusive exchange system) and DAS (Dispersed Alkaline Substrate) technologies for the passive treatment of high-strength acid mine drainage (AMD) from copper mining (pH~3, 633 mg Cu L−1). The chemical DAS and ADES prototypes showed the best performance in the removal of Cu, Al, and Zn (98–100%), while the biochemical SDES reactors achieved a high sulfate removal rate (average of 0.28 mol m−3 day-1). Notably, the DES technology was effective in protecting the sulfate-reducing communities from the high toxicity of the AMD, and also in maintaining bed permeability, an aspect that was key in the ADES reactor. The DAS reactor showed the highest reactivity, accumulating the metallic precipitates in a lower reactor volume, allowing to conclude that it requires the lowest hydraulic residence time among all the reactors. However, the concentration of precipitates resulted in the formation of a hardpan, which may trigger the need of removing it to avoid compromising the continuity of the treatment process. This study suggests the development of new treatment alternatives by combining the strengths of each technology in combined or serial treatments.

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Section: Microbiological and Molecular Analysismentioning

confidence: 99%

Evaluation of Dispersed Alkaline Substrate and Diffusive Exchange System Technologies for the Passive Treatment of Copper Mining Acid Drainage

Schwarz

Nancucheo

Gaete

et al. 2020

Water

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“…In many cases, the potential for acid generation greatly exceeds the neutralization potential of tailings, and liquors within and draining from tailing deposits can become highly acidic, and enriched with soluble transition metals derived from the dissolution of residual sulfidic (e.g., chalcopyrite; CuFeS 2 ) and other minerals. In addition, such waters are highly toxic to most life-forms [ 5 ]. The reactivity of pyritic mine tailings derives from their small particle size, and their content of acid-generating and metal-rich sulfidic minerals [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of an Iron-Reducing, Moderately Acidophilic Actinobacterium Isolated from Pyritic Mine Waste, and Its Potential Role in Mitigating Mineral Dissolution in Mineral Tailings Deposits

Nancucheo

Johnson

2020

Microorganisms

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Reactive pyritic mine tailings can be populated by chemolithotrophic prokaryotes that enhance the solubilities of many metals, though iron-reducing heterotrophic microorganisms can inhibit the environmental risk posed by tailings by promoting processes that are the reverse of those carried out by pyrite-oxidising autotrophic bacteria. A strain (IT2) of Curtobacterium ammoniigenes, a bacterium not previously identified as being associated with acidic mine wastes, was isolated from pyritic mine tailings and partially characterized. Strain IT2 was able to reduce ferric iron under anaerobic conditions, but was not found to catalyse the oxidation of ferrous iron or elemental (zero-valent) sulfur, and was an obligate heterotrophic. It metabolized monosaccharides and required small amounts of yeast extract for growth. Isolate IT2 is a mesophilic bacterium, with a temperature growth optimum of 30 °C and is moderately acidophilic, growing optimally at pH 4.0 and between pH 2.7 and 5.0. The isolate tolerated elevated concentrations of many transition metals, and was able to grow in the cell-free spent medium of the acidophilic autotroph Acidithiobacillus ferrooxidans, supporting the hypothesis that it can proliferate in acidic mine tailings. Its potential role in mitigating the production of acidic, metal-rich drainage waters from mine wastes is discussed.

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“…Acidic environments in which sulfidic minerals are subjected to bacterially-accelerated oxidative dissolution, contain significant concentrations of sulfate and transition metals [1]. The biodiversity of life forms that can grow in such waters comprise a large variety of different species distributed in all three domains.…”

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confidence: 99%

Acidiferrimicrobium australe gen. nov., sp. nov., an acidophilic and obligately heterotrophic, member of the Actinobacteria that catalyses dissimilatory oxido-reduction of iron isolated from metal-rich acidic water in Chile

González

Huber

Tindall

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic, metal-contaminated stream draining from an abandoned underground coal mine (Trongol mine), situated close to Curanilahue, Biobío Region, Chile. The isolate (USS-CCA1T) was demonstrated to be a heterotroph that catalysed under aerobic conditions the oxidation of ferrous iron and the reduction of ferric iron under anaerobic conditions, but not the oxidation of sulfur nor hydrogen. USS-CCA1T is a Gram-positive, motile, short rod-shaped, mesophilic bacterium with a temperature growth optimum at 30 °C (range 20–39 °C). It was categorized as an extreme acidophile growing between 1.7 and 4.5 and optimally at pH 3.0. The G+C content of the chromosomal DNA of the isolate was 74.1 mol%, which is highly related to Aciditerrimonas ferrireducens IC-180T , (the most closely related genus; 94.4 % 16S rRNA gene identity), and higher than other acidophilic actinobacteria. The isolate (USS-CCA1T) was shown to form a distinct 16S rRNA clade from characterized acidophilic actinobacteria, well separated from the genera Acidimicrobium , Ferrimicrobium , Ferrithrix , ‘Acidithrix’ and Aciditerrimonas . Genomic indexes (ANIb, DDH, AAI, POCP) derived from the USS-CCA1T draft genome sequence (deposited at DDBJ/ENA/GenBank under the accession WJHE00000000) support assignment of the isolate to a new species and a new genus within the Acidimicrobiaceae family. Isolate USS-CCA1T is the designated type strain of the novel species Acidiferrimicrobium australe (=DSM 106828T,=RGM 2506T).

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Performance of a sulfidogenic bioreactor inoculated with indigenous acidic communities for treating an extremely acidic mine water

Cited by 28 publications

References 31 publications

Evaluation of Dispersed Alkaline Substrate and Diffusive Exchange System Technologies for the Passive Treatment of Copper Mining Acid Drainage

Evaluation of Dispersed Alkaline Substrate and Diffusive Exchange System Technologies for the Passive Treatment of Copper Mining Acid Drainage

Characteristics of an Iron-Reducing, Moderately Acidophilic Actinobacterium Isolated from Pyritic Mine Waste, and Its Potential Role in Mitigating Mineral Dissolution in Mineral Tailings Deposits

Acidiferrimicrobium australe gen. nov., sp. nov., an acidophilic and obligately heterotrophic, member of the Actinobacteria that catalyses dissimilatory oxido-reduction of iron isolated from metal-rich acidic water in Chile

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