Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods

Choe, Hyun‐Sop; Lee, Dong Sup; Lee, Seung‐Ju; Hong, Sung-Hoo; Park, Dong Choon; Lee, Mi-Kyung; Kim, Tae‐Hyoung; Cho, Yong-Hyun

doi:10.1016/j.ijid.2013.07.011

Cited by 91 publications

(94 citation statements)

References 35 publications

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“…The formerly known U. urealyticum biovars, biovar 1 and biovar 2, were identified as separate species, U. parvum and U. urealyticum, respectively by polymerase chain reaction (PCR) (4). Ureaplasma spp.…”

Section: Introductionmentioning

confidence: 99%

“…with NGU depends on the species detected and that U. urealyticum, but not U. parvum, is an etiological agent in NGU (5). Furthermore, it has been reported that U. urealyticum can cause infections in the lower genital tract and is a pathogen of male urethritis (4,6). However, U. urealyticum has been reported as a probable cause of bacterial vaginosis, and there are limited data indicating that bacterial vaginosis might cause cervicitis in females (5).…”

Section: Introductionmentioning

confidence: 99%

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<i>Ureaplasma urealyticum</i>: Presence among Sexually Transmitted Diseases

et al. 2017

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SUMMARY:The aim of this study was to detect the presence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, and Ureaplasma urealyticum in genital specimens of symptomatic patients. This study also examined the role of U. urealyticum in infections of the lower genital tract. Cervical and urethral samples from 96 patients (46 males, 50 females) were tested using the Seeplex(  ) STD6 ACE kit. Consent forms were received and a questionnaire was applied. All statistical analyses were performed using the SPSS statistical software program (version 17.0). Among the samples tested, at least 1 pathogen was detected in 49z of the samples; specifically, the rate of detection of U. urealyticum, M. hominis, C. trachomatis, N. gonorrhoeae, M. genitalium, and T. vaginalis was 29.1z, 10.4z, 8.3z, 7.3z, 6.3z, and 4.2z, respectively. U. urealyticum was detected as the sole pathogen in samples from 10z of female patients and 28.3z of male patients (p ＝ 0.035). U. urealyticum was present in 54.5z (18/33) of samples in which a single pathogen was detected and 71.4z (10/14) of samples in which multiple pathogens were detected. Among men, significant differences in discharge, dysuria, and pruritus were not noted among those with negative results (84.6z, 69.2z, and 38.5z, respectively), among those positive for only U. urealyticum (100z, 66.7z, and 26.7z, respectively), and those positive for N. gonorrhoeae, C. trachomatis, M. genitalium, and T. vaginalis (100z, 93.3z, and 26.7z, respectively). Detection of U. urealyticum, either alone or together with other pathogens, in a symptomatic group of patients is an important finding, particularly in men.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<i>Ureaplasma urealyticum</i>: Presence among Sexually Transmitted Diseases

et al. 2017

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“…Polymerase chain reaction (PCR) is an advanced molecular biology method, presenting high sensitivity and rapidity compared to traditional methods (culture-based and other standard methods) (11,12). Quick detection of these pathogens is important and mul- (14). Simultaneous detection of multiple pathogens using PCR amplification presents higher sensitivity and specificity compared to reference methods (14,15 …”

Section: Introductionmentioning

confidence: 99%

“…Quick detection of these pathogens is important and mul- (14). Simultaneous detection of multiple pathogens using PCR amplification presents higher sensitivity and specificity compared to reference methods (14,15 …”

Section: Introductionmentioning

confidence: 99%

Distribution of sexually transmitted diseases in a group of symptomatic male patients using urine samples and PCR technique / Distribuția bolilor cu transmitere sexuală într-un grup de barbați simptomatici utilizand probe de urina si tehnica PCR

Vică

Junie

Grad

et al. 2015

Revista Romana De Medicina De Laborator

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“…Moreover, multiplex molecular assays have an additional advantage in screening and diagnosis since they enable simultaneous diagnosis of several sexually transmitted infections (STIs). Several commercial multiplex PCR kits have been developed, and a few studies have evaluated their performance …”

Section: Introductionmentioning

confidence: 99%

Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens

Huh

Yun

et al. 2018

Clinical Laboratory Analysis

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Background The DiaPlexQ™ STI6 Detection Kit (DiaPlexQ; Solgent Co., Ltd., Daejeon, South Korea) is a multiplex real‐time PCR assay for the detection of the following sexually transmitted disease (STD) pathogens: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Trichomonas vaginalis, Ureaplasma urealyticum, and Mycoplasma genitalium. We compared the performance of the DiaPlexQ assay with the GeneFinder™ STD I (CT/NG/UU) and STD II (MG/MH/TV) Multiplex Real‐time PCR Kits (GeneFinder; Infopia Co., Ltd., Anyang, South Korea). Methods We evaluated the performance of the DiaPlexQ assay in comparison to that of GeneFinder using 1106 clinical specimens (542 genital swabs and 564 urine samples). The analytical performance of the DiaPlexQ assay, including the limit of detection (LOD) and analytical specificity, was evaluated using reference strains. Results The positive percent agreement, negative percent agreement, and kappa value between the two assays were 96.6%‐99.4%, 98.2%‐99.8%, and 0.93%‐0.99%, respectively. No cross‐reactivity was observed in a collection of 41 different microorganisms and the LOD of the DiaPlexQ assay ranged from 1 to 10 copies/reaction for each microorganism. Conclusion The DiaPlexQ assay showed comparable performance to that of the GeneFinder assay so that it can be used for the screening and diagnosis of non‐viral curable STD pathogens.

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Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods

Abstract: Multiplex real-time PCR was found to be an equivalent or superior modality for the diagnosis of STIs. It could be a cost-effective and rapid diagnostic tool for the simultaneous detection of multiple STI microorganisms.

Cited by 91 publications

References 35 publications

<i>Ureaplasma urealyticum</i>: Presence among Sexually Transmitted Diseases

<i>Ureaplasma urealyticum</i>: Presence among Sexually Transmitted Diseases

Distribution of sexually transmitted diseases in a group of symptomatic male patients using urine samples and PCR technique / Distribuția bolilor cu transmitere sexuală într-un grup de barbați simptomatici utilizand probe de urina si tehnica PCR

Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens

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