2009
DOI: 10.5539/mas.v3n5p51
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Performance of CHROM Agar and Oxacillin Resistant Screening Agar Base Media for Detection of Methicillin Resistant Staphylococcus aureus (MRSA) from Chronic Wound

Abstract: CHROM agar Staphylococcus aureus and Oxacillin Resistant Screening Agar Base (ORSAB) media with oxacillin were evaluated for the screening of Methicillin Resistant Saphylococcus aureus (MRSA). Among 190 samples, totally 126 confirmed Staphylococcus aureus strains were used for screening of MRSA used CHROM agar and ORSAB media were compared with the other MRSA screening media like Baird Park agar (BPA) with ciprofloxacin, Mannitol salt agar (MSA) with oxacillin, Blood agar (BA) with oxacillin and Muller Hinton … Show more

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Cited by 4 publications
(4 citation statements)
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“…Thus, CHROM agar was found superior to the oxacillin screen agar method. Similar findings were also observed by Taguchi et al, (2004) and Karthy et al, (2009). This provides an alternative for the detection of MRSA in clinical laboratories, especially when PCR is unavailable.…”
Section: Phenotypic Identification Of Mrsa Isolatessupporting
confidence: 74%
“…Thus, CHROM agar was found superior to the oxacillin screen agar method. Similar findings were also observed by Taguchi et al, (2004) and Karthy et al, (2009). This provides an alternative for the detection of MRSA in clinical laboratories, especially when PCR is unavailable.…”
Section: Phenotypic Identification Of Mrsa Isolatessupporting
confidence: 74%
“…CHROMagar was found superior to the oxacillin screen agar methods for phenotypic detection of methicillin resistant isolates. Similar findings were also observed by Taguchi et al, (2004) and Karthy et al, (2009). Higher sensitivity of CHROM agar in this study might be attributed to the fact that CHROMagar with cefoxitin supplement was potent inducer of the mecA gene; this could explain why heterogeneous MRS populations that variably express the mecA gene are better detected by CHROMagar with cefoxitin than medium with oxacillin, which is a weak inductor of PBP2a production.…”
Section: Phenotypic Identification Of Mrcons Isolatessupporting
confidence: 87%
“…Both has advantages and disadvantages such as speed, reliability and acessibility. Phenotypic methods involves standard microbiological technique of S. aureus detection which include Gram staining, colonial morphorlogy, catalase and coagulase tests, pigment production and anaerobic growth (Karthy et al, 2009). Additional methods include Minimum Inhibitory Concentrations, methods that detect mecA gene or PBP2 0 protein and media containing oxacillin (Louie et al, 2001).…”
Section: Advances In Animal and Veterinary Sciences February 2015 | Vmentioning
confidence: 99%