Aim:Tropical theileriosis is fatal hemoprotozoal disease of dairy animals caused by Theileria annulata. The aim of the present study was to detect the T. annulata and comparison of results of molecular and microscopic techniques.Materials and Methods:A total of 52 blood samples were collected from the cattle suspected for theileriosis across the Banaskantha district. All the samples were screened for theileriosis using Giemsa’s staining technique and polymerase chain reaction (PCR).Results:Total of 17 (32.69%) and 24 (46.15%) samples were found positive for theileriosis by microscopic examination and PCR test, respectively. It revealed that the study area is endemic for theileriosis, and the microscopic technique has 70.83% sensitivity and 100% specificity with respect to PCR technique.Conclusion:It may be concluded from the present study that the PCR is comparatively sensitive technique than microscopic examination and may be recommended to use in the field for screening of theileriosis in the study area, where a high prevalence of diseases have been reported due to intensive dairy farming.

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Bovine Brucellosis is an infectious disease caused by Brucella abortus. It is a small, nonmotile, coccobacilli, Gram negative, facultative, intracellular bacterial organisms of the genus Brucella. Brucella is named after Sir David Bruce who, in 1886, first isolated the organisms from the spleen of a soldier at postmortem with what was then called Malta fever. The isolation of B. abortus from aborted cattle was carried out by Bang in 1897 (Mcmahan, 1944). The disease has a considerable impact on human and animal health, as well as socioeconomic impacts, especially in which rural income relies largely on livestock breeding and dairy products. It causes significant reproductive losses in sexually mature animals (Wadood et al., 2009). According to OlE, it is the second most

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Brucella abortus is generally known to cause brucellosis in cattle and buffalo. Here, we report the draft genome sequence of Brucella abortus SKN 13, isolated from aborted cattle placenta in the area of Gujarat, India, providing precious resources for comparative genomic analyses of Brucella field strains.