In recent past, the respiratory infection has emerged as a great challenge to the poultry farmers. Various pathogens including Avian pneumovirus (APV), Avian influenza virus (AIV), Infectious bronchitis virus (IBV) and Newcastle disease virus (NDV), Avibacterium paragallinarum, Ornithobacterium rhinotracheale (ORT), Mycoplasma synoviae (MS), Mycoplasma gallisepticum (MG) and Avian pathogenic Escherichia coli (APEC) are involved in the respiratory disease complex in birds [1], [2] (Bradbury, 1984; Roussan et al., 2008). Hence, respiratory disease complex is the most serious disease affecting to poultry and causes heavy economic losses in the poultry industry worldwide [3] (Murthy et al., 2008). In recent years, metagenomics is powerful analyzing tool for detection of pathogens directly from clinical samples without any prior knowledge of the organism in a given sample [4], [5] (Schuster, 2008; Pereira et al., 2010). High throughput Next-Generation-Sequencing technology was used for sequencing the isolated genomic DNA. These data provides an insight about taxonomic and functional status of microorganisms responsible for causing respiratory infection in broiler. The data of these metagenome are available in the BioSample Submission Portal as Bioproject PRJNA339659 and SRA accession number SRR5997823, SRR5992854, SRR6037376, SRR6024702, SRR6012248 and SRR6008913.

Aim:Tropical theileriosis is fatal hemoprotozoal disease of dairy animals caused by Theileria annulata. The aim of the present study was to detect the T. annulata and comparison of results of molecular and microscopic techniques.Materials and Methods:A total of 52 blood samples were collected from the cattle suspected for theileriosis across the Banaskantha district. All the samples were screened for theileriosis using Giemsa’s staining technique and polymerase chain reaction (PCR).Results:Total of 17 (32.69%) and 24 (46.15%) samples were found positive for theileriosis by microscopic examination and PCR test, respectively. It revealed that the study area is endemic for theileriosis, and the microscopic technique has 70.83% sensitivity and 100% specificity with respect to PCR technique.Conclusion:It may be concluded from the present study that the PCR is comparatively sensitive technique than microscopic examination and may be recommended to use in the field for screening of theileriosis in the study area, where a high prevalence of diseases have been reported due to intensive dairy farming.

| Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. To ascertain the serological status of the disease, a total of 1373 samples were screened for the presence of Brucella antibodies by RBPT and i-ELISA. Overall seroprevalence recorded in sheep was 6.84% (94 samples out of 1373) and 4.80% (66 out of 1373) by RBPT and i-ELISA respectively. Clinical status wise seroprevalence was recorded in heifer 4.65% and 6.97%, clinically healthy animals 2.62% and 1.79%,animals with the history of abortion 16.58% and 13.98%, hygroma 27.27% and 18.18%,pregnant 4.59% and 2.29%, non-pregnant 6.80% and 4.18%, status unknown 10.78% and 6.86%, still birth 17.64% and 5.88%, retention of placenta 20.00% and 20.00%,orchitis16.12% and 9.67% respectively by RBPT and i-ELISA, respectively. Breedwise highest seroprevalence was recorded in Patanwadi 7.82% and 5.99% followed by Marwadi 6.35% and 4.16%,Magra 3.84% and 1.28%, Avikalin 4.76% and 0.00%and Chokhla 1.78% and 0.00%by RBPT and i-ELISA, respectively. Present study revealed that the Patanwadi breed of the sheep is relatively more susceptible and high rate of seroprevalence was observed from the animals having history of abortion, retention of placenta and hygroma.

Brucella abortus is generally known to cause brucellosis in cattle and buffalo. Here, we report the draft genome sequence of Brucella abortus SKN 13, isolated from aborted cattle placenta in the area of Gujarat, India, providing precious resources for comparative genomic analyses of Brucella field strains.

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