Performance of diapausing parasitoid wasps, Habrobracon hebetor, after cold storage

Chen, Haoliang; Hong-yu, Zhang; Zhu, Kun Yan; Throne, James E.

doi:10.1016/j.biocontrol.2012.11.007

Cited by 28 publications

(31 citation statements)

References 34 publications

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“…This implies that temperature has a greater influence on expression of HhHsp70I than the insect's diapause status. For HhHsp70II , the transcript level was higher when H. hebetor were reared at 20°C and 10 : 14 L : D than when reared at 17.5°C and either 16 : 18 L : D or 10 : 14 L : D. We also found (Chen et al ., ) that H. hebetor perform best after long‐term cold storage when the wasp was reared at 20°C and a 10‐h photophase. Further study should be conducted on the relationship between this gene and cold tolerance in H. hebetor .…”

Section: Discussionmentioning

confidence: 98%

“…It has been used to suppress moth populations in stored products (Balevski, ; Brower & Press, ; Cline & Press, ; Garba & Gaoh, ; Huang, ; Press et al ., ) and in field crops (Cheng, ; Gerling, ; Imam et al ., ; Uwais et al ., ). Long‐term storage of H. hebetor would facilitate its use in biological control programs, and we have recently shown that adult females appear to enter reproductive diapause when reared at 17.5°C or 20°C at 10 : 14 L : D (Chen et al ., ) and that these females can be stored for up to 2 months without loss of vitality (Chen et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

et al. 2013

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Heat shock proteins (HSPs) are known as chaperones that help with folding of other proteins when cells are under environmental stresses. The upregulation of HSPs is essential for cold survival during insect diapause. The ectoparasitoid Habrobracon hebetor, a potential biological control agent, can enter reproductive diapause when reared at low temperature and short photoperiod. However, the expression of HSPs during diapause of H. hebetor has not been studied. In this study, we sequenced and characterized the full-length complementary DNAs of three Hsp70 genes (HhHsp70I, HhHsp70II and HhHsp70III) from H. hebetor. Their deduced amino acid sequences showed more than 80% identities to their counterparts from other insect species. However, the multiple sequence alignment among the three deduced amino acid sequences of HhHsp70s showed only 46% identities. A phylogenetic analysis of the three HhHsp70s and all other known Hsp70 sequences from Hymenoptera clustered all the Hsp70s into four groups, and the three HhHsp70s were distributed into three different groups. Real-time quantitative polymerase chain reaction analysis showed that the expression of the three HhHsp70 genes in H. hebetor reared at different conditions was quite different. HhHsp70I showed higher relative expression when H. hebetor were reared at 27.5°C than at two lower temperatures (17.5°C and 20°C) regardless of the photoperiod, whereas HhHsp70II showed higher expression when H. hebetor were reared at 20°C and 10 : 14 L : D than when reared at 17.5°C and either 16 : 8 L : D or 10 : 14 L : D. In contrast, HhHSP70III was expressed at similar levels regardless of the rearing conditions. These results may suggest functional differences among the three HhHsp70 genes in H. hebetor.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

et al. 2013

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“…However, another study that provided honey to naturally overwintered T. semistratus, showed fewer offspring from overwintered females [66]. A male-biased sex ratio from some offspring may be attributed to females that did not mate before transfer to an overwintering environment but also occurs after cold-storage in some parasitoids [67]. The Pacific Northwest's period of H. halys egg-laying between May and October means that T. japonicus may be in diapause for as few as five months.…”

Section: Parasitoid Longevity and Fecundity In Overwintering Habitatmentioning

confidence: 99%

Establishment in an Introduced Range: Dispersal Capacity and Winter Survival of Trissolcus japonicus, an Adventive Egg Parasitoid

Lowenstein

Andrews

Hilton

et al. 2019

Insects

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The herbivorous brown marmorated stink bug, Halyomorpha halys, has spread globally, and one of its key parasitoids, Trissolcus japonicus, has recently been detected in the pest's introduced range. For an exotic natural enemy to impact its targeted host in a novel environment, it must disperse, locate hosts, and potentially be redistributed to susceptible sites. Through intentionally releasing T. japonicus across four Oregon eco-regions, we investigated an introduced parasitoid's dispersal capacity in urban sites and in two perennial crops, hazelnut and raspberry. In a second paired field and laboratory study, we investigated T. japonicus survival in different plant materials. Within three days of release, adult T. japonicus located host egg masses at 45% of sites and, one year later, were detected at 40% of release sites. Areas where released wasps survived winter were mostly urban or semi-natural. In commercial crop release experiments, we recovered the highest percentage of wasps in raspberry within 5 m of the release site but found no statistical difference in dispersal distance with some wasps dispersing up to 50 m. Adult parasitoids survived up to 16 weeks outdoors in the winter, with greater survival over time in bark compared to leaf litter. Wasp survival remained above 50% over the course of a simulated winter environment without precipitation. Our work affirms the continuation of H. halys parasitism by T. japonicus in novel environments and provides insight into the high population sizes necessary to survive winter and locate host egg masses the following season.

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“…Exposure to low temperatures can affect physiological activity (Hance et al 2007), reducing the body parasitoid mass and, consequently, their longevity as reported for Trichogramma nerudai Pintureau and Gerding (Hymenoptera: Trichogrammatidae) (Tezze & Botto 2004). This is due to the need for resources during long cold storage periods (Chen et al 2013). In addition, absence of feeding and water loss during storage also may reduce parasitoid longevity (Chen et al 2011).…”

Section: Discussionmentioning

confidence: 98%

Biological Quality of Tetrastichus Howardi (Hymenoptera: Eulophidae) Reared with Tenebrio molitor (Coleoptera: Tenebrionidae) Pupae after Cold Storage

Tiago

Pereira

Kassab

et al. 2019

Florida Entomologist

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Performance of diapausing parasitoid wasps, Habrobracon hebetor, after cold storage

Cited by 28 publications

References 34 publications

Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

Establishment in an Introduced Range: Dispersal Capacity and Winter Survival of Trissolcus japonicus, an Adventive Egg Parasitoid

Biological Quality of Tetrastichus Howardi (Hymenoptera: Eulophidae) Reared with Tenebrio molitor (Coleoptera: Tenebrionidae) Pupae after Cold Storage

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