Performing the paradoxical: how plant peroxidases modify the cell wall

Passardi, Filippo; Penel, Claude; Dunand, Christophe

doi:10.1016/j.tplants.2004.09.002

Cited by 769 publications

(618 citation statements)

References 70 publications

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“…Tanto as APXs quanto as POXs apresentam várias isoformas distribuídas por diversos compartimentos celulares (Shigeoka et al 2002;Cavalcanti et al, 2007;Passardi et al 2004). Em conjunto, estas enzimas representam componentes importantes do sistema de proteção oxidativa nas folhas e nas raízes de arroz (Dionisio-Sese & Tobita 1998), milho (Azevedo-Neto et al 2006) e feijão caupi (Cavalcanti et al 2004;Cavalcanti et al 2007).…”

Section: Introductionunclassified

“…As POXs apresentam um caráter bifuncional no metabolismo oxidativo, pois podem utilizar o H 2 O 2 para oxidar vários substratos por meio do seu ciclo catalítico peroxidativo e também podem produzir OH · a partir do H 2 O 2 por intermédio do seu ciclo catalítico hidroxílico (Passardi et al 2004). Assim sendo, as POX de parede celular podem viabilizar o alongamento celular devido à produção de OH · e à clivagem não-enzimática dos polissacarídeos de ligação cruzada (Liszkay et al 2004).…”

Section: Introductionunclassified

“…Assim sendo, as POX de parede celular podem viabilizar o alongamento celular devido à produção de OH · e à clivagem não-enzimática dos polissacarídeos de ligação cruzada (Liszkay et al 2004). Por outro lado, estas enzimas também podem restringir o crescimento celular por meio do espessamento da parede celular em decorrência da eliminação do H 2 O 2 associada à polimerização dos precursores da lignina (Passardi et al 2004). Como conseqüência, as POX de parede celular podem estar envolvidas na regulação do crescimento celular envolvendo o metabolismo do H 2 O 2 .…”

Section: Introductionunclassified

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Atividade de enzimas antioxidantes e inibição do crescimento radicular de feijão caupi sob diferentes níveis de salinidade

et al. 2012

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ABSTRACT(Activities of antioxidant enzymes and root growth inhibition in cowpea seedlings exposed to diff erent salt levels). Phenol peroxidase (POX) is a dual enzyme that is involved with hydrogen peroxide scavenging and lignin biosynthesis, contributing to growth inhibition by secondary wall thickening. In order to relate growth inhibition to salt-induced oxidative modulation, the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and POX were evaluated in cowpea roots under salinity. Four-day-old seedlings of the Pitiúba and Pérola cultivars were exposed to 0, 25, 50, 75 and 100 mM NaCl in germination paper under controlled conditions. Aft er two days of treatment, root length was reduced under 100 mM NaCl by 56 and 26% in Pitiúba and Pérola, respectively, which was associated with enhanced electrolyte leakage and cell death in the root apex. NaCl salinity did not trigger lipid peroxidation, indicating that cell death was probably due to membrane damage instead of oxidative stress. Salt stress reduced the activity of SOD, CAT and APX and increased the POX activity, demonstrating that this enzyme plays a role in oxidative protection in cowpea roots exposed to NaCl salinity. In conclusion, salt-induced growth inhibition in cowpea roots could be attributed, at least in part, to a coordinate action involving an increase in POX activity and a drop in CAT and APX activities. Atividade de enzimas antioxidantes e inibição do crescimento radicular de feijão caupi sob diferentes níveis de salinidade

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Atividade de enzimas antioxidantes e inibição do crescimento radicular de feijão caupi sob diferentes níveis de salinidade

et al. 2012

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“…These enzymes catalyze the oxidation of a variety of electron donor substrate (e.g. phenols, aromatic amines) in the presence of hydrogen peroxide (Passardi et al 2004). Microbes and animal tissues represent a huge family of heme containing enzymes (Dunford 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have been done on aminoacid sequencing and heme structure of peroxidases (Welinder and Mazza 1977;Silva et al 1990). Peroxidase has been implicated in metabolic processes such as ethylene biogenesis, cell development and membrane integrity (Welinder and Mazza 1977), building and rigidification of cell walls (Passardi et al 2004), auxin catabolism (Delannoy et al 2004), senescence (Faivre-Rampant 1998), defense mechanism towards pathogens (Choi et al 2007;Passardi et al 2005) and various abiotic stresses, including metal ions and UV stress (Marcel et al 2001), salt (Yi and Lee 2003), air pollution damage (Lee 2002), cold tolerance (Tao et al 1998), control of cell elongation, polymerization of extension (Ahmed et al 1995), generation of reactive oxygen species (Bestwick et al 1998), hydrogen peroxide scaveng-ing (Kawaoka et al 2003), lignin polymerization (Fagerstedt et al 2010) and the suberization processes (Quiroga et al 2000;Kolattukudy 2001).…”

Section: Introductionmentioning

confidence: 99%

Biochemical characterization of Santalum album (Chandan) leaf peroxidase

Kumar¹,

Kamle²,

Singh³

2011

Physiol Mol Biol Plants

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The Santalum peroxidase was extracted from the leaves and precipitated with double volume of chilled acetone. The optimum percent relative activity for the Santalum peroxidase was observed at pH 5.0 and 50°C temperature. The Santalum peroxidase per cent relative activity was stimulated in the presence of phenolic compounds like ferrulic acid and caffeic acids; however, indole-3-acetic acid (IAA) and protocatechuic acid act as inhibitors. All divalent cations Fe 2+ , Mn 2+ , Mg 2+ , Cu 2+ and Zn 2+ stimulate the relative activity of the Santalum peroxidase at concentration of 2.0 μM. Amino acids like L-alanine and L-valine activate the per cent relative activity, while L-proline and DL-methionine showed moderate inhibition for the Santalum peroxidase. However, a very low a concentration of cysteine acts as a strong inhibitor of Santalum peroxidase at the concentration of 0.4 mM. Native polyacrylamide gel electrophoresis (Native-PAGE) was performed for isoenzyme determination and two bands were observed. K m and V max values were calculated from Lineweaver-Burk graph. The apparent V max /K m value for O-dianisidine and H 2 O 2 were 400 and 5.0×105 Units/min/ mL respectively.

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Genomics of Root Hairs

Cho

2012

Root Genomics and Soil Interactions

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Performing the paradoxical: how plant peroxidases modify the cell wall

Cited by 769 publications

References 70 publications

Atividade de enzimas antioxidantes e inibição do crescimento radicular de feijão caupi sob diferentes níveis de salinidade

Atividade de enzimas antioxidantes e inibição do crescimento radicular de feijão caupi sob diferentes níveis de salinidade

Biochemical characterization of Santalum album (Chandan) leaf peroxidase

Genomics of Root Hairs

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