Perfusion Chromatography: An Approach to Purifying Biomolecules

Afeyan, Noubar B.; Fulton, Scott P.; Gordon, Neal F.; Mazsaroff, István; Várady, László; Regnier, Fred E.

doi:10.1038/nbt0390-203

Cited by 101 publications

(35 citation statements)

References 9 publications

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“…Ž . zene Afeyan et al, 1990a , was higher in terms of shorter separation time than that of conventional HPLC with 5᎐10 m particles. With the former, the processing time was reduced by a factor of ten and the protein productivity Ž .…”

Section: Introductionmentioning

confidence: 77%

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Convection‐enhanced mass transfer in aggregated beads for gel chromatography

Grandmaison

Goosen

et al. 2000

AIChE Journal

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Section: Introductionmentioning

confidence: 77%

“…mer rods Vogt and Freitag, 1998 . A typical pore structure of macroporous beads consists of two sizes of pores, small ones allowing for molecular diffusion and large ones allowing Ž for through-pore convection Afeyan et al, 1990aAfeyan et al, , 1991Lloyd . and Warner, 1990;Lloyd, 1991;Frey et al, 1993 .…”

Section: Introductionmentioning

confidence: 99%

Convection‐enhanced mass transfer in aggregated beads for gel chromatography

Grandmaison

Goosen

et al. 2000

AIChE Journal

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“…These include continuous extraction (Pungor, 1987), affinity cross-flow filtration (Huang et al, 1988), radial-flow (Luong et al, 1987) and magnetically fluidized columns (Burns et al, 1985), affinity expanded beds (Chase and Draeger, 1992). and perfusion chromatography (Afeyan et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Breakthrough of lysozyme through an affinity membrane of cellulose‐cibacron blue

Liu

Fried

1994

AIChE Journal

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The dynamic affinity adsorption of lysozyme through stacked flat-sheet cellulose membranes with immobilized cibacron blue 3GA was studied and compared to three affinity-membrane models: diffusion model to explore the importance of axial and radial diffusion; variation model to study the effects of pore-size distribution or thickness variation; and stack model to investigate the effects of stacking flat-sheet membranes. For the diffusion model, when Per> 0.1, radial diffusional resistance is significant, but when Pe, < 25, axial dispersion must be considered. For the variation model, increasing pore-size distribution or nonuniform membrane thickness greatly broadens the breakthrough curve. The stack model shows that the stacking of membranes significantly sharpens the breakthrough curves by averaging out the flow dispersion due to pore-size distribution or thickness variation.

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“…Efforts to exploit this "flow-through" concept took many forms. One was to make the pores of the particle so large that some flow of mobile phase through particles would occur without compression, as in the case of perfusion chromatography where the pores range to 6,000 -8,000 Å in size [4]. Perfusible media are made by emulsion polymerization of organic monomers in the presence of suitable porogens that produce very large pores.…”

Section: Introductionmentioning

confidence: 99%