Perfusion of the isolated canine stomach

Abstract

artery can be drawn from the pulse or the pressure within it. SUMMARY I. Direct measurement of arterial flow is desirable. At present, electromagnetic flowmeters appear to be the best instruments for this, but are still beset with difficulties.2. Contrary to the usual assumption, pulse, pressure, and temperature are not directly related to arterial flow.3. 'Teflon' prostheses and vein grafts of a narrowing shape were inserted in dogs to by-pass arterial occlusions. Whether the blood entered at the wide or the narrow end did not make any appreciable difference to the resulting flows and pressures.4. The presence of a pulse beyond an arterial obstruction may have an apparently paradoxical relation to the distal flow. It has been confirmed by experiment in dogs that such a pulse may disappear as flow improves and distal pressure rises, and may reappear as flow decreases and distal pressure falls as the result of distal obstruction.

Potentiation between Pentagastrin and Bethanechol Chloride in Stimulation of Gastric Secretion from Isolated <i>ex vivo</i> Canine Stomach Perfused with Homologous Blood

“…We developed a method of maintaining the anatomic and functional viability of totally isolated canine stomachs perfused with homologous blood for 24 h [5][6][7][8][10][11][12]17]. We demonstrated that ex vivo canine stomachs, when adequately stimulated, always secrete HC1 during the perfusion with homologous blood of a living support ing dog.…”

Effect of Secretin on Mucinous Secretion by the Isolated Canine Stomach Perfused Extracorporeally

“…This preparation secretes H \ pepsin and various mucinous substances, when stimulated by cholinergic or humoral secrctagogucs and perfused with homologous, cross-circulated blood of a sup porting animal, serving as a living oxygenator and dialyser. The ex vivo secretion period can last as long as 24 h. A detailed description of our method of stomach dissection, ex vivo perfusion and secretion collection has been reported previously (15)(16)(17)(18)(19)(20), and will not be repeated here. During perfusion, arterial and portal venous pressure and arterial flow rate of gastric vessels were constantly monitored.…”

Hormonal control of the gastric motor response to feeding: Cross‐perfusion of an isolated stomach using a conscious donor dog

“…Traditionally, the accumulation of toxins has been prevented by using anesthetized donor dogs to cross-perfuse the isolated stomach and maintain its physiological responsiveness [8,9]. There are some disadvantages with this technique in studies of digestive function.…”