Perinatal and neonatal outcomes of 494 babies delivered from 972 vitrified embryo transfers

Shi, Wenhao; Xue, Xia; Zhang, Silin; Zhao, Weichen; Liu, Shan; Zhou, Hanying; Wang, Min; Shi, Juanzi

doi:10.1016/j.fertnstert.2012.02.051

Cited by 88 publications

(61 citation statements)

References 16 publications

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“…The pregnancy and perinatal outcomes observed in all four groups of our study, as a whole, are consistent with previous studies of day 3 vitrified-thawed embryo transfer [Liu et al 2013;Rama Raju 2009;Shi et al 2012]. In those studies, as in ours, no significant differences between regimens were observed in the mean gestational week, birth weight, ratio for singleton and twins, congenital birth defects rate, neonatal mortality rate, still birth rate, or cerebral palsy rate in singletons.…”

Section: Discussionsupporting

confidence: 92%

A modified natural cycle results in higher live birth rate in vitrified-thawed embryo transfer for women with regular menstruation

Guan

Fan

Styer

et al. 2016

Systems Biology in Reproductive Medicine

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(2016) A modified natural cycle results in higher live birth rate in vitrified-thawed embryo transfer for women with regular menstruation, Systems Biology in Reproductive Medicine, 62:5, 335-342, DOI: 10.1080/19396368.2016 To link to this article: https://doi.org/10. 1080/19396368.2016 There is no consensus regimen for the optimal endometrial preparation for cryopreservation and vitrified-thawed embryo transfer cycles. This is largely caused by the lack of sufficient investigation and analyses on the respective pregnancy and perinatal outcomes by different regimens. This study aimed to compare both pregnancy and perinatal outcomes between the modified natural and artificial cycles in vitrified-thawed day three embryo transfer for women with regular menstruation. A total of 1,482 vitrified-thawed day three embryo transfer cycles were reviewed including 427 modified natural cycles (NC), 132 ovulation induction cycles (OC), 794 artificial cycles (AC), and 129 GnRH agonist artificial cycles (GAC). The primary outcome that was evaluated was live birth rate. The NC regimen demonstrated a higher rate of ongoing pregnancy (43.8% vs. 30.2%, P = 0.002) and a lower rate of late abortion (2.8% vs. 14.0%, P = 0.003) than the GAC regimen as well as a higher implantation rate (31.9% vs. 27.1%, P = 0.008) and live birth rate (43.1% vs. 34.1%, P = 0.002) than the AC regimen. A significantly higher peak endometrial thickness before transfer was observed in patients using the NC and GAC regimens (10.0 ± 1.7, 9.9 ± 2.4) compared to the AC regimens (9.2 ± 1.5, P = 0.000). Multivariate logistic regression showed that the NC protocol was associated with a higher live birth rate. There were no significant differences in rates of pregnancy complications, neonatal mortality, birth defects, mean birth weight, and other perinatal outcomes among the regimens. Modified natural cycle endometrial preparation regimen for vitrified-thawed day three embryo transfer is associated with superior live birth pregnancy outcomes compared to artificial cycles. Future studies are warranted to investigate the underlying biologic mechanisms of these findings.Abbreviations ART: assisted reproductive technology; BMI: body mass index; FET: frozen-thawed embryo transfer; HCG: human chorionic gonadotropin; IVF: in-vitro fertilization; IVF-ET: in-vitro fertilization and embryo transfer; OHSS: ovarian hyperstimulation syndrome; RCTs: randomized controlled trials ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 92%

A modified natural cycle results in higher live birth rate in vitrified-thawed embryo transfer for women with regular menstruation

Guan

Fan

Styer

et al. 2016

Systems Biology in Reproductive Medicine

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“…Results from this study, along with those from previous studies, indicate that Cryotop vitrification of human embryos is satisfactory and relevant to clinical settings [2][3][4]21]. However, the major drawback of this procedure is the direct exposure of the biological samples to LN 2 , which carries a potential risk of cross-contamination.…”

Section: Discussionsupporting

confidence: 56%

“…Vitrification is a viable alternative to the conventional slowfreezing method used in assisted reproduction technology (ART), since it is associated with high embryo survival rates and successful outcomes [1][2][3][4]. Vitrification/warming solutions (VS/WS) typically contain high concentrations of protein, most frequently human serum albumin (HSA), to confer their cryoprotective effects.…”

Section: Introductionmentioning

confidence: 99%

Perinatal outcomes for transfer of blastocysts vitrified and warmed in defined solutions with recombinant human albumin: 374 babies born after 898 embryo transfers

Murakami

Egashira

Tanaka

et al. 2014

J Assist Reprod Genet

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Purpose To assess the efficacy of a novel, defined vitrification procedure using recombinant human albumin (rHA) for cryopreservation of human blastocysts. Design: Retrospective study. Setting: Private IVF clinic. Patients: 1,496 patients received vitrified/warmed embryo transfer (ET). Methods Surplus blastocysts, and blastocysts from patients undergoing elective embryo cryopreservation, were vitrified/ warmed using Cryotop carriers in homemade solutions containing either human serum albumin (HSA) or rHA. Main Outcome Measures: Clinical and neonatal outcomes regarding the vitrified/warmed ET procedures. Results The HSA and rHA groups had a total of 1,163 and 898 vitrified/warmed cycles, respectively. Embryo survival rates (98.7 % vs. 98.9 %, respectively) and the number of embryos transferred (1.08±0.01 vs. 1.06±0.01, respectively) were similar in the HSA and rHA groups. Clinical pregnancy rates/ET were higher (P<0.05) in the rHA group (56.0 %) than in the HSA group (51.5 %). The HSA and rHA groups had similar live delivery rates/pregnancy (72.2 % vs. 72.3 %, respectively) and perinatal outcomes, including birth weight (2,988±28 vs. 3,046±26 g, respectively). Birth defects occurred in 0.9 % and 1.6 % of neonates in the HSA and rHA groups, respectively. Conclusions rHA effectively replaced HSA for human embryo vitrification procedures, and yielded high rates of pregnancy and live births after vitrified/warmed ET. This new approach will support the development of defined ART systems, which will eliminate the variation and risks associated with the use of blood-derived products.

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“…Most published reports looking at the neonatal outcomes after transfer of vitrified embryos have used blastocyst-stage embryos (Takahashi et al, 2005;Mukaida et al, 2009;Wikland et al, 2010). Reported data on children born after transfer of Day 3 vitrified embryos are relatively rare (Rama Raju et al, 2009;Kato et al, 2012;Shi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Obstetric and neonatal outcomes after transfer of vitrified early cleavage embryos

Liu

Teng

et al. 2013

Human Reproduction

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what is known already: Vitrification has been successfully used for cryopreserving human oocytes and blastocyst-stage embryos. Most published studies looking at the neonatal outcomes after transfer of vitrified embryos refer to blastocyst-stage embryos. Information on children born after transfer of Day 3 vitrified embryos is relatively rare. study design, size, duration: A retrospective, single-centre study of children born after Day 3 embryo transfer from fresh, slow frozen or vitrified embryos during the period January 2006 to May 2011 was conducted. Each patient contributed only one cycle per group. Children born were followed-up at 7-30 days after delivery. Outcome measures include obstetric and neonatal outcomes, which were evaluated by medical records and questionnaires sent to parents. main results and the role of chance: Frequencies of hypertensive disorder, gestational diabetes, placenta previa and abruptio placenta were similar in all groups. Five hundred and forty five, 986 and 1914 singleton babies were born from vitrified, slow freezing and fresh transfers, and the median gestational ages were 38.7, 38.7 and 38.7 weeks, respectively. Preterm birth (32 -37 weeks) occurred in 7.5, 9.2 and 7.8% of the vitrified, slow freeze and fresh groups, respectively. The median birthweight from vitrified embryos (3455.3 g) was higher than that from slow freezing (3352.3 g) and fresh (3355.8 g) transfers (P , 0.0001 for both). The rate of perinatal mortality was 0.4% for all transfer groups. Three hundred and eighty two, 734 and 1322 twin babies were born from vitrified, slow freezing and fresh transfers, respectively. There were no differences among groups in mean gestational age and in the rate of preterm birth. The median birthweight for babies born from vitrified embryos (2587.4 g) was higher than that from the slow freezing (2538.8 g) or fresh (2494.4 g) transfer groups (vitrified versus fresh: P ¼ 0.0015; vitrified versus slow freeze: P ¼ 0.049). The rate of low birthweight (1500-2500 g) from vitrified (30.4%) was lower than that from fresh (36.2%) transfer (P ¼ 0.034).limitations, reasons for caution: The main limitation of this study is that the obstetric and neonatal data were obtained by questionnaires sent to the parents without checking medical records. This is, especially, problematic for reporting on congenital malformations, so birth defects were excluded from the data.wider implications of the findings: Transfer of vitrified and warmed Day 3 embryos does not seem to have an adverse effect on neonatal outcome. Children born following the transfer of vitrified embryos seem to have a higher birthweight when compared with those of fresh or slow frozen embryos. study funding/competing interest(s): This study received no outside funding and none of the authors has any conflict of interest.

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Perinatal and neonatal outcomes of 494 babies delivered from 972 vitrified embryo transfers

Cited by 88 publications

References 16 publications

A modified natural cycle results in higher live birth rate in vitrified-thawed embryo transfer for women with regular menstruation

A modified natural cycle results in higher live birth rate in vitrified-thawed embryo transfer for women with regular menstruation

Perinatal outcomes for transfer of blastocysts vitrified and warmed in defined solutions with recombinant human albumin: 374 babies born after 898 embryo transfers

Obstetric and neonatal outcomes after transfer of vitrified early cleavage embryos

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