Peripheral Neuritis Trauma in Pigs: A Neuropathic Pain Model

Castel, David; Sabbag, Itai; Brenner, Ori; Meilin, Sigal

doi:10.1016/j.jpain.2015.09.011

Cited by 25 publications

(46 citation statements)

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“…Models of cutaneous inflammatory pain, using capsaicin and UV-B irradiation have been used, demonstrating thermal and mechanical hyperalgesia in pigs (142, 143). Most recently, the choice of the pig as model for preclinical research has led to the characterization of postsurgical pain through the quantification of mechanical nociceptive thresholds (116, 144). Similarly, nociceptive stimuli delivered with an infrared diode laser have been employed to evoke withdrawal responses in the investigation of the efficacy of a novel analgesic (145).…”

Section: Pain Assessment In Pigsmentioning

confidence: 99%

A Review of Pain Assessment in Pigs

et al. 2016

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There is a moral obligation to minimize pain in pigs used for human benefit. In livestock production, pigs experience pain caused by management procedures, e.g., castration and tail docking, injuries from fighting or poor housing conditions, “management diseases” like mastitis or streptococcal meningitis, and at parturition. Pigs used in biomedical research undergo procedures that are regarded as painful in humans, but do not receive similar levels of analgesia, and pet pigs also experience potentially painful conditions. In all contexts, accurate pain assessment is a prerequisite in (a) the estimation of the welfare consequences of noxious interventions and (b) the development of more effective pain mitigation strategies. This narrative review identifies the sources of pain in pigs, discusses the various assessment measures currently available, and proposes directions for future investigation.

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Section: Pain Assessment In Pigsmentioning

confidence: 99%

A Review of Pain Assessment in Pigs

et al. 2016

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“…A technician carried each animal in their hands and placed an anesthetic facemask (Fritz Stephan GmbH Medizintechnik, Gackenbach, Germany) on the pig’s mouth and nose, as described previously. 5 Each animal was anesthetized with a 3% isoflurane/100% oxygen mixture. The pig was placed in the sternal position on the operating table.…”

Section: Methodsmentioning

confidence: 99%

“…However, even though rodent models for pain continue to provide valuable data on the mechanisms driving pain, 3 , 4 the use of these models in drug development is criticized due to limited translational ability to human conditions. 5 – 10 …”

Section: Introductionmentioning

confidence: 99%

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Open field and a behavior score in PNT model for neuropathic pain in pigs

Castel¹,

Sabbag²,

Nasaev³

et al. 2018

JPR

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BackgroundRodent models are frequently used in the research of pain and continue to provide valuable data on the mechanisms driving pain, although they are criticized due to limited translational ability to human conditions. Previously we have suggested pigs as a model for development of drugs for neuropathic pain. In this study, we investigate the spontaneous behavior of pigs following peripheral neuritis trauma (PNT)-induced neuropathic pain.MethodsA computerized monitoring system was used to evaluate the changes in open field test in addition to applying a composite behavior scoring system. The data suggest that the PNT operation did not affect the animal’s ability to walk as the total distance walked by PNT animals was not significantly different from the total distance walked by sham-operated animals. However, PNT animals expressed a significant change in the pattern of walking. This effect was unrelated to the time that the animals spent in the open field. Following treatment with different drugs (morphine, buprenorphine, or gabapentin), the walking pattern of the animals in the open field changed in a drug-specific manner. In addition, the detailed behavior score revealed drug-specific changes following treatment.ResultsPharmacokinetic analysis of the drug concentration in blood and cerebrospinal fluid correlated with the behavioral analysis.ConclusionThe data of this study suggest that the open field test together with the detailed behavior score applied in this model are a powerful tool to assess the spontaneous behavior of pigs following PNT-induced neuropathic pain.

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“…4 Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family that is upregulated following peripheral nerve injury. [5][6][7][8][9][10] Following synthesis in small-to medium-sized dorsal root ganglion (DRG) neurones, 9 BDNF is anterogradely transported to the dorsal horn where it is released upon noxious stimulation in an activity-dependent manner. 11 The physiological actions of BDNF are mediated via interaction with two receptors, the high affinity tyrosine receptor kinase B (TrkB) and p75NTR, also known as low-affinity nerve growth factor receptor (LNGFR).…”

Section: Introductionmentioning

confidence: 99%