Peritoneal Dialysis Catheter Increases Leukocyte Recruitment in the Mouse Parietal Peritoneum Microcirculation and Causes Fibrosis

Kowalewska, Paulina M.; Margetts, Peter J.; Fox‐Robichaud, Alison

doi:10.3747/pdi.2014.00211

Cited by 7 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This increase in mtDNA levels at the very inception of PD could reflect an acute response of the peritoneum to tissue injury caused by the catheter insertion. This increase in the level of free mtDNA could be a consequence of leukocyte rolling and extravasation by peritoneal catheter insertion, acting as a foreign body [ 48 ]. In addition, there was no difference in mtDNA levels between percutaneous and surgical placement (data not shown), although, in this particular case, our analysis was hampered by a limited statistical power.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial Dysfunction Plays a Relevant Role in Pathophysiology of Peritoneal Membrane Damage Induced by Peritoneal Dialysis

et al. 2021

View full text Add to dashboard Cite

Preservation of the peritoneal membrane is an essential determinant of the long-term outcome of peritoneal dialysis (PD). Epithelial-to-mesenchymal transition (EMT) plays a central role in the pathogenesis of PD-related peritoneal membrane injury. We hypothesized that mitochondria may be implicated in the mechanisms that initiate and sustain peritoneal membrane damage in this setting. Hence, we carried out ex vivo studies of effluent-derived human mesothelial cells, which disclosed a significant increase in mitochondrial reactive oxygen species (mtROS) production and a loss of mitochondrial membrane potential in mesothelial cells with a fibroblast phenotype, compared to those preserving an epithelial morphology. In addition, in vitro studies of omentum-derived mesothelial cells identified mtROS as mediators of the EMT process as mitoTEMPO, a selective mtROS scavenger, reduced fibronectin protein expression induced by TGF-ß1. Moreover, we quantified mitochondrial DNA (mtDNA) levels in the supernatant of effluent PD solutions, disclosing a direct correlation with small solute transport characteristics (as estimated from the ratio dialysate/plasma of creatinine at 240 min), and an inverse correlation with peritoneal ultrafiltration. These results suggest that mitochondria are involved in the EMT that human peritoneal mesothelial cells suffer in the course of PD therapy. The level of mtDNA in the effluent dialysate of PD patients could perform as a biomarker of PD-induced damage to the peritoneal membrane.

show abstract

Section: Discussionmentioning

confidence: 99%

Mitochondrial Dysfunction Plays a Relevant Role in Pathophysiology of Peritoneal Membrane Damage Induced by Peritoneal Dialysis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The reason why these studies overestimate the potential for human applications is primarily related to the reduced dimensions of the animal under investigation. If one inserts the NPs into the liver of mice, an organ which for humans is outside the detection range, the maximum detected irradiance is of the order of 10 -4 W/cm 2 (assuming similar values for the attenuation coefficients and the dimensions of tissues provided in literature [196][197][198] ) under 330-mW/cm 2 excitation. Such a value is way above the lower detection limit of currently available cameras.…”

Section: Pathways To Address the "Tissue Attenuation" Issuementioning

confidence: 92%

Quo Vadis, Nanoparticle-Enabled In Vivo Fluorescence Imaging?

et al. 2021

View full text Add to dashboard Cite

The exciting advancements that we are currently witnessing in terms of novel materials and synthesis approaches are leading to the development of colloidal nanoparticles (NPs) with increasingly greater tunable properties. We have now reached a point where it is possible to synthesize colloidal NPs with functionalities tailored to specific societal demands. The impact of this new wave of colloidal NPs has been especially important in the field of biomedicine. In that vein, luminescent NPs with improved brightness and near-infrared working capabilities have turned out to be optimal optical probes that are capable of fast and high-resolution in vivo imaging. However, luminescent NPs have thus far only reached a limited portion of their potential. Although we believe that the best is yet to come, the future might not be as bright as some of us think (and have hoped!). In particular, translation of NP-based fluorescence imaging from preclinical studies to clinics is not straightforward. In this Perspective, we provide a critical assessment and highlight promising research avenues based on the latest advances in the fields of luminescent NPs and imaging technologies. The disillusioned outlook we proffer herein might sound pessimistic at first, but we consider it necessary to avoid pursuing “pipe dreams” and redirect the efforts toward achievableyet ambitiousgoals.

show abstract

“…Subsequently, mPMCs were incubated in serum-free medium for 12 h and then cultured with normal glucose (NG) or 4.25% d -glucose (HG) with or without Tamo (10 nM) for 48 h, followed by evaluation of E-cadherin, Vimentin, and GSK-3β expression. mPMCs were incubated for 12 h with NG, HG, or HG+Tamo in the absence or presence of TWS (1 μM) to inhibit GSK-3β activity, followed by evaluation of β-catenin, p-β-catenin, and Snail expression [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Tamoxifen attenuates dialysate-induced peritoneal fibrosis by inhibiting GSK-3β/β-catenin axis activation

et al. 2018

View full text Add to dashboard Cite

Peritoneal fibrosis is a severe complication arising from long-term peritoneal dialysis (PD). Tamoxifen (Tamo) has been clinically proven effective in a series of fibrotic diseases, such as PD-associated encapsulating peritoneal sclerosis (EPS), but the mechanisms underlying Tamoxifen’s protective effects are yet to be defined. In the present study, C57BL/6 mice received intraperitoneal injections of either saline, 4.25% high glucose (HG) PD fluid (PDF) or PDF plus Tamoxifen each day for 30 days. Tamoxifen attenuated thickening of the peritoneum, and reversed PDF-induced peritoneal expression of E-cadherin, Vimentin, matrix metalloproteinase 9 (MMP9), Snail, and β-catenin. Mouse peritoneal mesothelial cells (mPMCs) were cultured in 4.25% glucose or 4.25% glucose plus Tamoxifen for 48 h. Tamoxifen inhibited epithelial-to-mesenchymal transition (EMT) as well as phosphorylation of glycogen synthase kinase-3β (GSK-3β), nuclear β-catenin, and Snail induced by exposure to HG. TWS119 reversed the effects of Tamoxifen on β-catenin and Snail expression. In conclusion, Tamoxifen significantly attenuated EMT during peritoneal epithelial fibrosis, in part by inhibiting GSK-3β/β-catenin activation.

show abstract

Peritoneal Dialysis Catheter Increases Leukocyte Recruitment in the Mouse Parietal Peritoneum Microcirculation and Causes Fibrosis

Cited by 7 publications

References 31 publications

Mitochondrial Dysfunction Plays a Relevant Role in Pathophysiology of Peritoneal Membrane Damage Induced by Peritoneal Dialysis

Mitochondrial Dysfunction Plays a Relevant Role in Pathophysiology of Peritoneal Membrane Damage Induced by Peritoneal Dialysis

Quo Vadis, Nanoparticle-Enabled In Vivo Fluorescence Imaging?

Tamoxifen attenuates dialysate-induced peritoneal fibrosis by inhibiting GSK-3β/β-catenin axis activation

Contact Info

Product

Resources

About