Peritoneal Macrophages

Daems, Walter

doi:10.1007/978-1-4899-5031-4_3

Cited by 14 publications

(12 citation statements)

References 181 publications

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“…FITC-dextran (FITC-D), used in combined fluorescence stereo microscopy, fluorescence microscopy, and electron microscopy, was employed as tracer to study the endocytic qualities of MLC (Thorball, 1981). Our results indicate that MLC to some extent resemble resident peritoneal macrophages as described by Carr (1967), Hirsch andFedorko (1970), Carr and Daems (1980), and Daems (1980). Mice were used as experimental animals as the muscle layers of the small intestine are of a relatively simple construction.…”

mentioning

confidence: 53%

Macrophage‐like cells in the muscularis externa of mouse small intestine

et al. 1985

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In muscularis externa of mouse small intestine, cells with ultrastructural features of macrophages were invariably observed in three layers: in the subserosal layer, between the circular and longitudinal muscle layers, and in association with the deep circular plexus. These macrophage-like cells (MLC) had a single indented nucleus, perinuclear Golgi complex, smooth and rough endoplasmic reticulum, many pits (coated and uncoated) in the plasma membrane, coated vesicles, light vesicles, and primary lysosomes, but rather few heterogeneous lysosomal vacuoles. MLC were partially enveloped by processes of interstitial cells of Cajal. FITC-dextran used in combined fluorescence stereo microscopy, fluorescence microscopy, and electron microscopy was employed as a tracer to study the endocytic qualities of the MLC. The mice were killed 5, 15, 30, and 60 min, 1 day, and 4 days after dextran administration. By fluorescence microscopy after 1 or 4 days MLC were observed as a constant cellular population with a strikingly regular distribution. By electron microscopy dextran-containing vacuoles were conspicuous after 1 h or more. MLC of the subserosal layer and between the circular and longitudinal muscle layers could be distinguished with respect to general appearance, pattern formation, and apparent dextran contents.

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mentioning

confidence: 53%

Macrophage‐like cells in the muscularis externa of mouse small intestine

et al. 1985

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“…Resident macrophages were obtained from newborn and adult albino rats (Holtzman, Madison, WI) by lavage ofthe peritoneal cavity (Daems, 1980), and monocytes by a Ficoll hypaque gradient (Mosier, 1981). Figure I.…”

Section: Cell Culturesmentioning

confidence: 99%

Characterization of ameboid microglia isolated from developing mammalian brain

Giulian¹,

Baker²

1986

J. Neurosci.

1,233

882

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Ameboid microglia are isolated from the cerebral tissue of neonatal rat by selective cell adhesion to plastic. Histochemical markers show that the microglial preparations are homogeneous (95 +/- 3%) and represent a 10% yield from starting cultures. Isolated ameboid microglia contain nonspecific esterase activity, the macrophage surface antigens MAC-1 and MAC-3, and acetylated low-density lipoprotein receptors. Ameboid cells have functional properties similar to those of macrophages, including the ability to engulf 5 micron latex beads, to secrete Interleukin-1 (IL-1) and to release superoxide anion. Unlike monocytes and adherent spleen cells, ameboid microglia do not show peroxidase activity by histochemical stain. Unlike resident peritoneal macrophages, ameboid microglia proliferate in vitro. Scanning electron microscopy shows that ameboid cells have short, spinous processes that can be distinguished from the ruffled surfaces of body macrophages. Our observations suggest that ameboid microglia are a distinct class of mononuclear phagocytic cells. Retinoic acid and dimethyl sulfoxide, agents known to accelerate differentiation in vitro, stimulate ameboid cells to develop thin processes several hundred microns in length. These “process-bearing” microglia eventually lose the capacity to engulf latex beads and to proliferate. They also show reductions in nonspecific esterase activity and in the binding of acetylated low- density lipoprotein. We suggest that in vitro ameboid microglia differentiate into nonphagocytic cells similar to ramified microglia found in normal adult brain. The isolation techniques described here provide the opportunity to study the composition and function of different microglial subpopulations during the development of the CNS.

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“…However, after the initial influx of circulating blood monocytes, there also frequently appears to be an induction of mitotic activity in local macrophages. Daems (1980) has suggested that under steady-state conditions, blood monocytes rapidly migrate through the vasculature of tissues and organs for the purposes of surveillance, and only under inflammatory or other insult conditions are retained in the tissues and differentiate into macrophages in order to assist in the activities of the resident tissue macrophages. An obvious implication of this idea is that it potentially allows the relatively immature and adaptable circulating blood monocyte to differentiate into a macrophage whose functional properties are determined by the prevailing conditions (e.g., stimuli such as bacteria, activated complement components, connective tissue breakdown products, oxygen tension, etc.)…”

Section: Monocyte Influx Under Inflammatory Conditionsmentioning

confidence: 99%

Macrophage Involvement in Wound Repair, Remodeling, and Fibrosis

Riches

1988

The Molecular and Cellular Biology of Wound Repair

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Peritoneal Macrophages

Cited by 14 publications

References 181 publications

Macrophage‐like cells in the muscularis externa of mouse small intestine

Macrophage‐like cells in the muscularis externa of mouse small intestine

Characterization of ameboid microglia isolated from developing mammalian brain

Macrophage Involvement in Wound Repair, Remodeling, and Fibrosis

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