Permanent gold nanoparticle coatings on polyelectrolyte multilayer modified capillaries for open-tubular capillary electrochromatography

Qu, Qishu; Liu, Dengping; Mangelings, Debby; Yang, Chun; Hu, Xiaoyun

doi:10.1016/j.chroma.2010.08.057

Cited by 51 publications

(22 citation statements)

References 47 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The efficient separation of acidic and basic proteins was achieved using didodecyldimethylammonium bromide (DDAB), bilayer-protected AuNPs as dynamic coating additives [21]. Recently, a modified capillary wall by the ionic adsorption of AuNPs on a polyelectrolyte multilayer (PEM) was reported as a new medium for [22].…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle‐coated capillaries for protein and peptide analysis on open‐tubular capillary electrochromatography

2012

View full text Add to dashboard Cite

We report a new method of immobilization of gold nanoparticles (AuNPs) on a fused-silica capillary through covalent binding. The resulting modified capillary was applied to electrophoretic systems to improve the efficiency of separation and the selectivity of selected solutes. The immobilization of AuNPs on the capillary wall was performed in a very simple and fast way without requiring heating. The surface features of an AuNP-coated capillary column were determined using the scanning electron microscopy. The chromatographic properties of AuNP-coated capillaries were investigated through variation of the buffer pH and separation voltage. Effective separations of synthetic peptides mixture were obtained on the AuNP-coated capillaries. The method shows a remarkable stability since it was reused about 900 times. The capacity factor was duplicated. Therefore, this modification is stable and can be applied to different separation purposes. A complex mixture of tryptic peptide fragments of HSA was analyzed in both the bare- and the AuNP-coated capillaries. Better electrophoretic peptide profile was observed when using the AuNP-coated capillary.

show abstract

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle‐coated capillaries for protein and peptide analysis on open‐tubular capillary electrochromatography

2012

View full text Add to dashboard Cite

show abstract

“…[18][19][20][21][22] A bare fused-silica capillary was activated with 1 M NaOH. A solution of 7.5% (w/w) PDDAC in Tris-HCl buffer (pH 8.0) was pumped through the capillary for 30 min, followed by rinsing with water for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Hence, the electrostatic interaction between ionic polymers and charged nanocrystals can give a stable immobilization onto the capillary surface. 21 In this study, cationic PDDAC was first selected as the binding layer between the CCND nanocrystals and the fused-silica surface.…”

Section: Immobilization Of the Ccnd Nanocrystals Onto The Capillary Smentioning

confidence: 99%

“…Such a stable dispersion may be due to a relatively high zeta potential on the surfaces of the organic nanocrystals, [17][18][19] which is advantageous for immobilizing onto the base material surfaces by an electrostatic layer-by-layer (LbL) deposition technique. 20,21 Oikawa et al reported that the LbL deposition of cationic poly(diallyldimethylammonium chloride) (PDDAC) and negatively-charged poly(diacetylene) nanocrystal dispersion has been successfully applied to the fabrication of multilayered thin films. 18,19,22 By utilizing these unique characteristics of the organic nanocrystals, the water-insoluble native CCND is expected to be electrostatically immobilized onto the capillary surface easily and stably without any organic synthetic processes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Open-tubular Electrochromatographic Chiral Separation of Amino Acids Using an Organic Nanocrystals Immobilized Capillary

et al. 2013

View full text Add to dashboard Cite

“…Using the 0.05% PEO modified DDAB-capped AuNPs dynamically coated capillary at pH 3.5, the separation of acidic and basic proteins featured high peak efficiencies and good reproducibility. Hu and coworkers applied a permanent coating approach, in which the gold nanoparticles were deposited on the silica surface via ionic adsorption to a polyelectrolyte multilayer (PEM) [64]. The first coating was constructed layer by layer with positively charged poly(diallydimethylammonium chloride), negatively charged poly(sodium-4-styrenesulfonate), and positively charged AuNPs.…”

Section: Nanomaterials Layersmentioning

confidence: 99%

Recent advances in column coatings for capillary electrophoresis of proteins

Hajba

Guttman

2017

TrAC Trends in Analytical Chemistry

121

View full text Add to dashboard Cite

Capillary coatings effectively improve the separation performance of proteins in capillary electrophoresis, mainly by reducing protein adsorption onto the inner capillary wall and by regulating the electroosmotic flow (EOF) to accommodate the separation problem in hand. In the first part of this review the newest trends in dynamic and permanent capillary coatings are summarized and discussed in detail. In the second part the application of nanomaterials as novel capillary coating materials is conversed. Nanomaterials have great potential in capillary coating preparations based on their advantageous properties such as large surface-to-volume ratios and a wide variety of chemistry options. Finally, some future prospective of capillary coatings in the emerging field of proteomics are given.

show abstract

Permanent gold nanoparticle coatings on polyelectrolyte multilayer modified capillaries for open-tubular capillary electrochromatography

Cited by 51 publications

References 47 publications

Gold nanoparticle‐coated capillaries for protein and peptide analysis on open‐tubular capillary electrochromatography

Gold nanoparticle‐coated capillaries for protein and peptide analysis on open‐tubular capillary electrochromatography

Open-tubular Electrochromatographic Chiral Separation of Amino Acids Using an Organic Nanocrystals Immobilized Capillary

Recent advances in column coatings for capillary electrophoresis of proteins

Contact Info

Product

Resources

About