Permeability of Intestinal and Blood—Tissue Barriers in Rats for Evans Blue Dye under Conditions of Acute Intoxication with Cyclophosphamide

Schäfer, Timur V; Ivnitsky, Ju. Ju.; Rejniuk, V. L.

doi:10.1007/s10517-019-04640-8

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…Intestinal inflammation often leads to impaired intestinal barrier function and increased gut permeability, which can exacerbate immune responses, disrupt the gut microbiota, and potentially result in systemic infections and mortality, particularly in neonate calves [ 53 , 54 ]. Additionally, colostrum is rich in beneficial probiotic bacteria, such as Lactobacillus and Bifidobacterium , which have been shown to, directly and indirectly, impact intestinal health [ 55 , 56 ]. These probiotics can modulate mucus production, maintain tight junctions between epithelial cells, and influence immunological mucosal tolerance through the IL-10/regulatory T cell axis [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Understanding the Immunomodulatory Effects of Bovine Colostrum: Insights into IL-6/IL-10 Axis-Mediated Inflammatory Control

Grigalevičiūtė

Matusevičius

Plančiūnienė

et al. 2023

Veterinary Sciences

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Bovine colostrum (COL), the first milk secreted by lactating cows postpartum, is a rich source of bioactive compounds that exert a significant role in the survival, growth, and immune development of neonatal calves. This study investigated the immunomodulatory effects of COL on cytokine production in vitro using a Caco-2/THP-1 macrophage co-culture model stimulated with Phorbol 12-myristate 13-acetate (PMA). COL pretreatment significantly reduced IL-6 (241.3 pg/mL) production induced by PMA (p < 0.05), while increasing IL-10 production (45.3 pg/mL), in comparison to PMA control (441.1 and 12.5 pg/mL, respectively). Further investigations revealed that the IL-6 suppressive effect of colostrum was heat-sensitive and associated with components of higher molecular mass (100 kDa). Moreover, colostrum primarily influenced THP-1 macrophages rather than Caco-2 epithelial cells. The effects of colostrum on IL-6 production were associated with reduced NF-κB activation in THP-1 macrophages. In calf-FMT transplanted C57BL/6 murine model, colostrum decreased intestinal permeability, reduced immune cell infiltration and intestinal score, and suppressed IL-6 (142.0 pg/mL) production during S. typhimurium infection, in comparison to control animals (215.2 pg/mL). These results suggest the immunomodulatory activity of bovine colostrum and its potential applications in inflammatory disorders. Further studies are needed to elucidate the underlying mechanisms and validate the findings in bovine models.

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Section: Discussionmentioning

confidence: 99%

Understanding the Immunomodulatory Effects of Bovine Colostrum: Insights into IL-6/IL-10 Axis-Mediated Inflammatory Control

Grigalevičiūtė

Matusevičius

Plančiūnienė

et al. 2023

Veterinary Sciences

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“…Intestinal inflammation often leads to impaired intestinal barrier function and in-creased gut permeability, which can exacerbate immune responses, disrupt the gut microbiota, and potentially result in systemic infections and mortality, particularly in neonate calves [51,52]. Additionally, colostrum is rich in beneficial probiotic bacteria, such as Lactobacillus and Bifidobacterium, which have been shown to directly and indirectly impact intestinal health [53,54]. These probiotics can modulate mucus production, maintain tight junctions between epithelial cells, and influence immunological mucosal tolerance through the IL-10/regulatory T cell axis [55].…”

Section: Discussionmentioning

confidence: 99%

Understanding the Immunomodulatory Effects of Bovine Colostrum: Insights into IL-6/IL-10 Axis-Mediated Inflammatory Control

Grigalevičiūtė

Matusevičius

Plančiūnienė

et al. 2023

Preprint

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Bovine colostrum (COL), the first milk secreted by lactating cows postpartum, is a rich source of bioactive compounds that exert significant role on the survival, growth, and immune development of neonatal calves [9,10]. This study investigated the immunomodulatory effects of COL on cytokine production in vitro using a Caco-2/THP-1 macrophage co-culture model stimulated with Phorbol 12-myristate 13-acetate (PMA). COL pretreatment significantly reduced IL-6 production induced by PMA, while increasing IL-10 production. Further investigations revealed that the IL-6 suppressive effect of colostrum was heat-sensitive and associated with components of higher molecular mass (100 kDa). Moreover, colostrum primarily influenced THP-1 macrophages rather than Caco-2 epithelial cells. The effects of colostrum on IL-6 production were associated with reduced NF-κB activation in THP-1 macrophages. In calf-FMT transplanted C57BL/6 murine model, colostrum decreased intestinal permeability, reduced immune cell infiltration, and suppressed IL-6 production during S. typhimurium infection. These results highlight the immunomodulatory activity of bovine colostrum and its potential therapeutic applications in inflammatory disorders. Further studies are needed to elucidate the underlying mechanisms and validate the findings in bovine models.

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“…Dye EB 8 (960.8 g/mol), jejunal loop injection, PCP [56] Blood Spectrophotometry at λ = 620 nm Extent of tissue damage can be visualized macroscopically. After administration, some tissues stain strongly stained so that are useless for histological evaluation [56].…”

Section: From the Intestinal Lumen Toward The Bloodmentioning

confidence: 99%

“…EB 7 (960.8 g/mol), I.V. infusion, PCP [56,59] Intestinal tissue Spectrophotometry at λ = 620 nm Relatively simple and affordable assay. EA dye is altered by the concentration of BSA in blood that passes through the epithelial tissue.…”

Section: Marker (Mw) 1 Administration Route and Permeability Pathway...mentioning

confidence: 99%

Role of Stress on Driving the Intestinal Paracellular Permeability

Molotla-Torres,

Guzmán-Mejía,

Godínez-Victoria

et al. 2023

CIMB

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The gut epithelium is a polarized monolayer that exhibits apical and basolateral membrane surfaces. Monolayer cell components are joined side by side via protein complexes known as tight junction proteins (TJPs), expressed at the most apical extreme of the basolateral membrane. The gut epithelium is a physical barrier that determinates intestinal permeability, referred to as the measurement of the transit of molecules from the intestinal lumen to the bloodstream or, conversely, from the blood to the gut lumen. TJPs play a role in the control of intestinal permeability that can be disrupted by stress through signal pathways triggered by the ligation of receptors with stress hormones like glucocorticoids. Preclinical studies conducted under in vitro and/or in vivo conditions have addressed underlying mechanisms that account for the impact of stress on gut permeability. These mechanisms may provide insights for novel therapeutic interventions in diseases in which stress is a risk factor, like irritable bowel syndrome. The focus of this study was to review, in an integrative context, the neuroendocrine effects of stress, with special emphasis on TJPs along with intestinal permeability.

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Permeability of Intestinal and Blood—Tissue Barriers in Rats for Evans Blue Dye under Conditions of Acute Intoxication with Cyclophosphamide

Cited by 4 publications

References 8 publications

Understanding the Immunomodulatory Effects of Bovine Colostrum: Insights into IL-6/IL-10 Axis-Mediated Inflammatory Control

Understanding the Immunomodulatory Effects of Bovine Colostrum: Insights into IL-6/IL-10 Axis-Mediated Inflammatory Control

Understanding the Immunomodulatory Effects of Bovine Colostrum: Insights into IL-6/IL-10 Axis-Mediated Inflammatory Control

Role of Stress on Driving the Intestinal Paracellular Permeability

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