Peroxidase activity in calluses and cell suspension cultures of radishRaphanus sativus var. Cherry Bell

Jeong

et al. 2022

IJMS

δ-Viniferin is a resveratrol dimer that possesses potent antioxidant properties and has attracted attention as an ingredient for cosmetic and nutraceutical products. Enzymatic bioconversion and plant callus and cell suspension cultures can be used to produce stilbenes such as resveratrol and viniferin. Here, δ-viniferin was produced by bioconversion from trans-resveratrol using conditioned medium (CM) of grapevine (Vitis labruscana) callus suspension cultures. The CM converted trans-resveratrol to δ-viniferin immediately after addition of hydrogen peroxide (H2O2). Peroxidase activity and bioconversion efficiency in CM increased with increasing culture time. Optimized δ-viniferin production conditions were determined regarding H2O2 concentration, incubation time, temperature, and pH. Maximum bioconversion efficiency reached 64% under the optimized conditions (pH 6.0, 60 °C, 30 min incubation time, 6.8 mM H2O2). In addition, in vitro bioconversion of trans-resveratrol was investigated using CM of different callus suspension cultures, showing that addition of trans-resveratrol and H2O2 to the CM led to production of δ-viniferin via extracellular peroxidase-mediated oxidative coupling of two molecules of trans-resveratrol. We thus propose a simple and low-cost method of δ-viniferin production from trans-resveratrol using CM of plant callus suspension cultures, which may constitute an alternative approach for in vitro bioconversion of valuable molecules.

Section: Bioconversion Of Trans-resveratrol To δ-Viniferin Through Cm...mentioning

confidence: 94%

Highly Efficient Bioconversion of trans-Resveratrol to δ-Viniferin Using Conditioned Medium of Grapevine Callus Suspension Cultures

Jeong

et al. 2022

IJMS

“…Cherry Bell derived from root, stem and leaf explants. Callus induction was carried with several combinations of benzyladenine (BA) and 2,4-D concentrations in agar MS medium and found that the callus obtained showed significantly greater peroxidase specific activities than those obtained from the native plant [12].…”

Section: B Peroxidase Activity In Native and Callus Culture Ofmentioning

confidence: 99%

Peroxidase Activity in Native and Callus Culture of Moringa Oleifera Lam

Shank¹,

Riyathong²,

Lee³

et al. 2013

JOMB

In vitro Moringa oleifera Lam. seedlings were successfully produced through seed culture on Murashige & Skoog (MS) agar medium containing 3% (w/v) sucrose and 0.2% (w/v) Gelrite TM in the absence of growth regulators under 1,500 lux of light density, 16 hour photoperiod light at temperature of 25 ± 2°C. Shoot-derived callus and rootderived callus of M. oleifera were established via culture of shoot and root on the MS medium supplemented with 0.5 mg/l of 2,4-dichlorophenoxy acetic acid (2,4-D) in the dark at 25 ± 2°C. Stem, leaf and root of native M. oleifera and M. oleifera callus were assayed for peroxidase activity using guaiacol as a substrate of the enzyme. In native plant, crude extract from root provided the highest peroxidase specific activity, followed by those from stem and leaf with the specific activity of 19.73 ± 0.18, 16.56 ± 1.43 and 13.38 ± 1.04 unit/mg protein, respectively. Crude extract of root-derived callus and shoot-derived callus of M. oleifera possessed specific activity of 167.25 ± 16.12 and 103.99 ± 10.64 unit/mg protein. These values are significantly higher than their counter parts from native M. oleifera suggesting the potential use of the callus cultures as new and improved sources of peroxidase.

“…In fact, the results of this study showed that the increase in the levels of growth regulators improved the protein content and the antioxidant enzyme activity. Moreno et al [53] studied the peroxidase activity in the callus culture of radish (Raphnus sativus cv. Cherry Bell).…”

Section: Discussionmentioning

confidence: 99%

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Dehestani-Ardakani¹,

Hejazi²,

Aliabad

2020

Mol Biol Rep

Purple coneflower (Echinacea purpurea (L.) Moench) is a widely used medicinal and ornamental plant. In the present study, the callus embryogenesis was examined using benzyl adenine (BA) at three levels (3, 4, 5 mg L−1), 1-Naphthalene acetic acid (NAA) at three levels (0.1, 0.2 and 0.5 mg L−1) with or without activated charcoal (1 g L−1), coconut milk (50 ml L−1) and casein hydrolysate (50 mg L−1) in the MS (Murashige and Skoog 1962) medium. The embryogenesis indirectly occurred with the production of callus. The calli were observed in three forms: undifferentiated, embryogenic and organogenic. The embryogenic calli were dark green and coherent with a faster growth rate. The highest embryogenesis (100%) and embryonic regeneration (plantlet production) were obtained in the combined BA + NAA treatments with the activated charcoal, coconut milk and casein hydrolysate. However, the combined treatments of growth regulators failed to produce somatic embryos without the use of coconut milk and casein hydrolysate. The maximum amount of protein, peroxidase and catalase activity of embryogenic calli (2.02, 1.79 and 6.62ΔOD/Min/mg.protein, respectively), and highest percentage of acclimatization success (29.3% of plants) were obtained in the combined treatment of 5 mg L−1 BA + 0.5 mg L−1 NAA + activated charcoal + coconut milk + casein hydrolysate. The highest amount of chlorophyll content (33.3 SPAD value) and growth characteristics of acclimatized plantlets were observed in the media containing 3 mg L−1 BA + 0.1 and 0.2 mg L−1 NAA + 1 g. L−1 combined activated charcoal, coconut milk, casein hydrolysate. The histological studies confirmed the somatic embryogenesis in purple coneflower. Generally, it was found that the somatic embryogenesis of E. purpurea occurs at high levels of BA and low levels of NAA with the addition of coconut milk and casein hydrolysate.