Perpendicular contact guidance of CNS neuroblasts exhibited on artificial microstructures

Nagata, Isao; Nakatsuji, Norio; Kawana, Akio

doi:10.1016/0921-8696(91)90893-r

Cited by 36 publications

(50 citation statements)

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“…Indirect patterning of the silicone surface as generated by molding guided neurites of both cell types in parallel to the grooves, as also observed earlier in various other studies on PC-12 cells 30,31 and primary isolated neuronal cells such as CNS neuroblasts 25 and hippocampal cells. 35 However, in our study microstructures generated by direct laser ablation did not influence the direction of neurite outgrowth in either of the two cell types.…”

Section: Discussionmentioning

confidence: 55%

“…However, a few studies found perpendicular orientations. 23,25 Therefore we compared the neuronal-like cell line PC-12 [30][31][32] with the primary SGCs isolated from the inner ear. Thus, surface structures were manufactured that could guide neuronal cell growth in the direction of the platinum contact material and could be used to minimize the distance between the neuronal cells and the electrode contact.…”

Section: Introductionmentioning

confidence: 99%

“…15 Further optimization of the electrode-neuron interface could be achieved by directing the orientation of neurons, especially in central auditory prosthesis with close contact to the neurons, such as AMI 16,17 and ABI. 18 Parallel microgrooves on surfaces of various materials had been shown to promote the alignment of neuronal cells, [19][20][21][22][23][24][25] astrocytes, 26,27 as well as fibroblasts. 28,29 The preferred orientation of neuronal cells is parallel to the microgrooves.…”

Section: Introductionmentioning

confidence: 99%

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Directing neuronal cell growth on implant material surfaces by microstructuring

et al. 2012

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CitationDirecting neuronal cell growth on implant material surfaces by microstructuring. 2012, 100 (4) Abstract: For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants. V C 2012 Wiley Periodicals, Inc.J Biomed Mater Res Part B: Appl Biomater 00B: 000-000, 2012.

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Directing neuronal cell growth on implant material surfaces by microstructuring

et al. 2012

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“…For tissue engineering applications, the micron-scale geometry of the surface of regeneration guidance channels influences the outcome of peripheral nerve regeneration, potentially by affecting the early arrangement of the fibrin matrix and/or inducing different cellular responses. 148 Nagata et al 149 cultured dissociated neuroblasts on quartz plates with grating-like microstructures fabricated by lithographic techniques. Various types of CNS neuroblasts, but not PNS neurons, oriented their processes and migrated both perpendicular and parallel to the axis of the microstructure.…”

Section: Vb Neuronal Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…Many works are concentrated on beach placers (Angusamy and Rajamanikcam, 2000, Cherien, 2003, Mohan, 2001) however, only limited work has been carried out for tsunami placer mineral studies. Worldwide tsunami impacts of the last century have been documented by many researchers (Heck, 1947;Iida et al, 1967;Nakata et al, 1993;Lander and Whiteside, 1997;NGDC, 2001and Shi et al 1995, Maramai et.al., 2005McMurtry et. al., 2004;Scheffers and Kelletat, 2003;Tappin et.al., 2001;Nanayama et.…”

Section: Introductionmentioning

confidence: 99%