Persistence and growth of faecal culturable bacterial indicators in water column and sediments of Vidy Bay, Lake Geneva, Switzerland

Poté, John; Haller, Laurence; Kottelat, Régis; Sastre, Vincent; Arpagaus, Philippe; Wildi, Walter

doi:10.1016/s1001-0742(09)60012-7

Cited by 67 publications

(51 citation statements)

References 31 publications

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“…The sediments of the Bay of Vidy are subject to extensive microbial contamination due to three identified sources including WWTP outlet discharge, the Chamberonne and Flon rivers (Goldscheider et al, 2007;Haller et al, 2009a, b;Poté et al, 2009). The inferred age of the sediment samples allows to assess the effect of WWPT effluent water on time distribution of E. coli and ENT in proximity points and also to identify the processes (such as accumulation of organic matter and lake eutrophication) that influenced the spatiotemporal variability of FIB in lake sediments (Thevenon et al, 2011a).…”

Section: Discussionmentioning

confidence: 99%

“…According to the sediment physico-chemical characteristics, the FIB can accumulate and develop in the aquatic environmental compartment (Haller et al, 2009b;Poté et al, 2009). The relative amount of OM and nutrients in sediments have a strong effect on the persistence and growth of FIB and may result in different degrees of survival (LaLiberte and Grimes, 1982;Craig et al, 2004;Poté et al, 2009;Haller et al, 2009b).…”

Section: Discussionmentioning

confidence: 99%

“…The negative (without DNA) and positive controls (e.g. the expected 520 bp length (for HF183/Bac708) from sewage (Poté et al, 2009)) were used for each PCR assays.…”

Section: Pcr Assays For Detection Of General Fib E Faecalis E Faementioning

confidence: 99%

“…Sediments may contain 100-1000 times as many FIB as the overlying water (Davies et al, 1995). FIB can survive longer in sediments than in the water column since sediments are enriched in organic compounds and thereby provide favorable nutrient conditions (Gerba and McLeod, 1976;LaLiberte and Grimes, 1982;Poté et al, 2009), and also a protection from sunlight inactivation (Sinton et al, 1999) and protozoan grazing (Davies and Bavor, 2000). The resuspension of FIB and pathogens from the sediments to the water column due to human activities or natural processes may contribute to potential human health risk Craig et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the evaluation of FIB in current sediments may represent a more stable index of overall or long-term water quality than in the overlying water (LaLiberte and Grimes, 1982;Ferguson et al, 2005;Haller et al, 2009b;Pachepsky and Shelton, 2011). The presence of FIB including E. coli and ENT, which affects water quality and safety, can originate from a variety of human and non-human sources also indicates the potential for the presence of pathogenic organisms Poté et al, 2009). It has been further suggested that human fecal material is generally considered to be of greater risk to human health as it is more likely to contain human pathogens (Scott et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of fecal indicator bacteria in sediments cores from the largest freshwater lake of Western Europe (Lake Geneva, Switzerland)

Thevenon

Regier

Benagli

et al. 2012

Ecotoxicology and Environmental Safety

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a b s t r a c tThis study characterized the fecal indicator bacteria (FIB), including Escherichia coli (E. coli) and Enteroccocus (ENT), disseminated over time in the Bay of Vidy, which is the most contaminated area of Lake Geneva. Sediments were collected from a site located at $ 500 m from the present waste water treatment plant (WWTP) outlet pipe, in front of the former WWTP outlet pipe, which was located at only 300 m from the coastal recreational area (before 2001). E. coli and ENT were enumerated in sediment suspension using the membrane filter method. The FIB characterization was performed for human Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium) and human specific bacteroides by PCR using specific primers and a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Bacterial cultures revealed that maximum values of 35.2 Â 10 8 and 6.6 Â 10 6 CFU g À 1 dry sediment for E. coli and ENT, respectively, were found in the sediments deposited following eutrophication of Lake Geneva in the 1970s, whereas the WWTP started operating in 1964. The same tendency was observed for the presence of human fecal pollution: the percentage of PCR amplification with primers ESP-1/ESP-2 for E. faecalis and E. faecium indicated that more than 90% of these bacteria were from human origin. Interestingly, the PCR assays for specifichuman bacteroides HF183/HF134 were positive for DNA extracted from all isolated strains of sediment surrounding WWPT outlet pipe discharge. The MALDI-TOF MS confirmed the presence of general E. coli and predominance E. faecium in isolated strains. Our results demonstrated that human fecal bacteria highly increased in the sediments contaminated with WWTP effluent following the eutrophication of Lake Geneva. Additionally, other FIB cultivable strains from animals or adapted environmental strains were detected in the sediment of the bay. The approaches used in this research are valuable to assess the temporal distribution and the source of the human fecal pollution in aquatic environments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The negative (without DNA) and positive controls (e.g. the expected 520 bp length (for HF183/Bac708) from sewage (Poté et al, 2009)) were used for each PCR assays.…”

Section: Pcr Assays For Detection Of General Fib E Faecalis E Faementioning

confidence: 99%