Persulphate challenge in female hairdressers with nasal hyperreactivity suggests immune cell, but no IgE reaction

Diab, Kerstin Kronholm; Truedsson, Lennart; Albin, María; Nielsen, Jörn

doi:10.1007/s00420-008-0392-3

Cited by 24 publications

(60 citation statements)

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“…The results of our study, with an increased expression of IL5 among symptomatic hairdressers compared to hairdressers without work-related bleaching powderassociated symptoms, suggest an immune-dependent mechanism. However, the results do not indicate an IgE-dependent response since the symptomatic hairdressers neither demonstrated increased levels of IL13 nor had signs of specific IgEs towards persulphate in skin prick testing and immunoblotting (Kronholm Diab et al 2009). This is in accordance with a challenge study on individuals with isocyanate-related asthma (Jones et al 2006).…”

Section: Discussionmentioning

confidence: 73%

“…Forty-one women were included in the original study by Kronholm Diab et al 2009. In this study, the inclusion criteria were successful RNA extraction and amplification, which was achieved from 19 of the subjects (Table 1) from the original study by Kronholm Diab et al 2009.…”

Section: Study Subjectsmentioning

confidence: 99%

“…In this study, the inclusion criteria were successful RNA extraction and amplification, which was achieved from 19 of the subjects (Table 1) from the original study by Kronholm Diab et al 2009. Those 19 subjects belonged to three groups: the target group consisted of female hairdressers with work-related nasal symptoms mainly related to bleaching powder (S).…”

Section: Study Subjectsmentioning

confidence: 99%

“…Bleaching powder contains persulphates known to elicit hypersensitive reactions from the airways (Moscato and Galdi 2006) and persulphate may be associated with nasal symptoms in hairdressers (Kronholm Diab et al 2009). However, the mechanism is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Among hairdressers who could point out a causative agent for their airway symptoms, 80 -90% named bleaching powder (Albin et al 2002;Leino et al 1998). Bleaching powder contains persulphates known to elicit hypersensitive reactions from the airways (Moscato and Galdi 2006) and persulphate may be associated with nasal symptoms in hairdressers (Kronholm Diab et al 2009). However, the mechanism is largely unknown.…”

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confidence: 99%

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Gene expression in nasal lavage from hairdressers exposed to persulphate

Jönsson

Broberg

Paulsson

et al. 2009

Int Arch Occup Environ Health

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ObjectivesMany hairdressers experience work-related symptoms from the airways caused by bleaching powder. This contains persulphates, which could be irritating to the mucous membrane and also may evoke an allergic reaction. However, specific IgE antibodies are difficult to detect.We found earlier that hairdressers with work-related bleaching powder-associated nasal symptoms reacted to persulphate, but that atopics also did and that the mechanism appeared to be similar in the two groups. In this study, we analysed gene expression of cytokines in the nose in order to further investigate the mechanism for work-related bleaching powderassociated nasal symptoms. MethodsThe study subjects belonged to either hairdressers with work-related bleaching powderassociated nasal symptoms (S; n=6), hairdressers without work-related bleaching powderassociated symptoms (WS; n=7) or atopics (A; n=6). Nasal lavage was performed before and during (up to 4 hours after the last challenge) provocation with potassium persulphate.Expression of two genes involved in allergic inflammation [interleukin 5 (IL5) and IL13] and one involved in cell-mediated immunity (interferon-gamma; IFNG) were analysed in nasal lavage with quantitative PCR. ResultsThe change of IL5 in the S group differed when compared to the WS group (P=0.051), in the A group when compared to the WS group (P=0.014), but not in the S group when compared to the A group (P=0.82). The change of IL13 in the A group differed when compared to the S (P=0.041) and WS (P=0.014) groups, but no difference was noticed between the S and WS 3 groups (P=0.30). The relative level of IFNG increased from before challenge to during challenge in the S group (P=0.031). ConclusionsSymptomatic hairdressers showed increased expression of IL5 and IFNG, but not IL13, during challenge. Hairdressers without work-related bleaching powder-associated nasal symptoms showed no markedly changed reaction. Atopics showed increased expression of IL5 and IL13.Thus, this may indicate a difference in the mechanism of symptoms between symptomatic hairdressers and atopics. However, due to the low number of participants, further studies are needed to elucidate the mechanism for persulphate-associated nasal symptoms.Keywords Cytokines, Gene expression, Interferon, Interleukin, Persulphate 4 Introduction Work-related airways symptoms, especially rhinitis, are very common in hairdressers (Albin et al. 2002;Brisman et al. 2003;Leino et al. 1997Leino et al. , 1998. Among hairdressers who could point out a causative agent for their airway symptoms, 80 -90% named bleaching powder (Albin et al. 2002;Leino et al. 1998).Bleaching powder contains persulphates known to elicit hypersensitive reactions from the airways (Moscato and Galdi 2006) and persulphate may be associated with nasal symptoms in hairdressers (Kronholm Diab et al. 2009). However, the mechanism is largely unknown. Skin prick testing reveals few cases of sensitisation (Aalto-Korte and Mäkinen-Kiljunen 2003;Hytönen et al. 1997;Leino et al. 1998;Moscato et al. 2005...

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Section: Discussionmentioning

confidence: 73%

Section: Study Subjectsmentioning

confidence: 99%

Section: Study Subjectsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Gene expression in nasal lavage from hairdressers exposed to persulphate

Jönsson

Broberg

Paulsson

et al. 2009

Int Arch Occup Environ Health

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The use of fractional exhaled nitric oxide in investigation of work‐related cough in a hairdresser

Pala

Pignatti

Moscato

2011

American J Industrial Med

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Strategy for identification and detection of multiple oxidative modifications within proteins applied on persulfate‐oxidized hemoglobin and human serum albumin

Mörtstedt

Jeppsson

Ferrari

et al. 2010

Rapid Comm Mass Spectrometry

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Oxidative stress has been suggested as an underlying mechanism of many human diseases. However, definitive evidence for this association has not been presented due to different shortcomings of the methods used to measure biomarkers of oxidative stress. Persulfates are oxidizing agents known to elicit hypersensitive reactions from the airways and skin. Despite a frequent use of persulfates at many work places, no biomarkers for persulfate exposure are available. The aim of this study was to develop a strategy for the identification and detection of multiple oxidative modifications within proteins. This strategy was applied on persulfate-oxidized proteins to identify oxidized peptides suitable for further investigation as biomarkers of persulfate exposure or oxidative stress. A strategy for the identification and the relative quantification of multiple oxidative modifications within proteins was developed. The usage of two software packages facilitated the search for modified peptides to a great extent. Oxidized peptides were relatively quantified using liquid chromatography/tandem mass spectrometry in selected reaction monitoring mode. The result showed that persulfates oxidize tryptophans and methionines resulting in mass shifts of 16 and/or 32 Da. Also, oxidized albumin peptides in nasal lavage fluid samples from subjects challenged with persulfate were detected. The oxidation degree before and after challenge remained constant for peptides containing methionine sulfoxide. For peptides containing oxidized tryptophan the oxidation degree increased after exposure. Some of these oxidized peptides may be suitable as biomarkers; however, further evaluation is required.

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Persulphate challenge in female hairdressers with nasal hyperreactivity suggests immune cell, but no IgE reaction

Abstract: Persulphate challenge affects hairdressers with BAR, but also atopics. The reaction may be driven by a Th1 cell activation.

Cited by 24 publications

References 24 publications

Gene expression in nasal lavage from hairdressers exposed to persulphate

Gene expression in nasal lavage from hairdressers exposed to persulphate

The use of fractional exhaled nitric oxide in investigation of work‐related cough in a hairdresser

Strategy for identification and detection of multiple oxidative modifications within proteins applied on persulfate‐oxidized hemoglobin and human serum albumin

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