this study aims to further develop the 14 c-pMMA porosity calculation method with a novel autoradiography technique, the Micro-pattern gas detector autoradiography (MpGDA). in this study, the MpGDA is compared with phosphor screen autoradiography (SpA). A set of rock samples from Martinique island exhibiting a large range of connected porosities was used to validate the MpGDA method. calculated porosities were found to be in agreement with ones from the SpA and the tripleweight method (TW). The filmless nature of MPGDA as well as straightforward determination of C-14 radioactivity from the source rock makes the porosity calculation less uncertain. The real-time visualization of radioactivity from C-14 beta emissions by MPGDA is a noticeable improvement in comparison to SpA. Autoradiography has demonstrated its adaptability and reliability in several research areas. With regard to the development of autoradiographic techniques the very first autoradiograph was obtained by Becquerel in 1896, through exposition of an emulsion of silver halide crystals in contact with radioactive material 1. Autoradiographic techniques allow the production of spatial distribution of radioactivity in the material; the sample itself being the source of the radioactivity either as occurring naturally in the material or as artificially added radioactivity. In the case of geological samples, 238 U, 232 Th and 40 K bearing minerals are the main sources of natural radioactivity 1. The radioactive source may also be in the form of an artificial radioactive marker, such as 3 H or 14 C labelled methylmethacrylate (MMA) 2-4. Investigated samples are impregnated with the radiolabeled tracer and fixed in place via polymerization of the marked MMA in the connected pore network of rock samples. Thus, the image of the connected pore network can be produced by exposing the impregnated sample surface on a photographic film. This brief description corresponds to the 14 C polymethylmethacrylate (14 C-PMMA) autoradiography method 2-4 and will be used for porosity analysis in this work. In the field of nuclear waste management, the 14 C-PMMA method has been successfully applied to investigate the connected pore network of geological materials, radioelements diffusion within rock matrices and relations between the porosity and mineralogy of rocks 5-8. In the biological field, injecting the radioligands into living tissues allows to map the anatomical location of specific receptors by autoradiographic techniques. For instance, Larsson & Ullberg 9 introduced a new autoradiographic technique, the whole body macro autoradiography (WBA), allowing to study the distribution of 35 S-labelled penicillin in a rat using entire frozen sections of them after injection of radiolabeled tracer. In drug distribution studies, Young and Kuhar 10 were able to localize opioid receptors in a rat brain by 14 C, 3 H, 125 I and 35 S, and more recently, Fang et al. 11 studied the effect of cocaine, ketamine and methamphetamine on dopamine terminals in the striatum of rats using 18 ...