2018
DOI: 10.1039/c8dt01568f
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pH dependent binding in de novo hetero bimetallic coiled coils

Abstract: a Herein the first example of a bimetallic coiled coil featuring a lanthanide binding site is reported, opening opportunities to exploit the attractive NMR and photophysical properties of the lanthanides in multi metallo protein design. In our efforts to fully characterise the system we identified for the first time that lanthanide binding to such sites is pH dependent, with optimal binding at neutral pH, and that the double AsnAsp site is more versatile in this regard than the single Asp site. Our second site… Show more

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Cited by 9 publications
(9 citation statements)
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“…Similar observations were made for the MB1-2 peptide, although the pH range through which Tb 3+ was bound was considerably larger (pH 4 -7). 119…”
Section: Lanthanide Binding Sites Using Common Amino Acidsmentioning
confidence: 99%
See 1 more Smart Citation
“…Similar observations were made for the MB1-2 peptide, although the pH range through which Tb 3+ was bound was considerably larger (pH 4 -7). 119…”
Section: Lanthanide Binding Sites Using Common Amino Acidsmentioning
confidence: 99%
“…130 Only one example of a lanthanide-containing hetero-bimetallic coiled coil has thus far been reported. 119 The coiled coil features a lanthanide binding Asp 3 site as well as a thiolate Cys 3 site, suitable for binding softer metal ions including mercury. 90,131 The soft thiol ligands contrast with the hard oxygen donors associated with lanthanide-binding, and provide a mechanism by which selectivity can be readily achieved based on hard-soft acid base theory.…”
Section: Heterometallic Coiled Coils Containing D-and F-block Metalsmentioning
confidence: 99%
“…Although Ln 3+ coiled coils display selectivity for Ln 3+ ions over Ca 2+ , [7c–f] there have been no studies relating to selectivity across the lanthanide series. Given the desire to distinguish between the different lanthanide ions, and the reports of coiled coils capable of metal ion discrimination based on size, [14] the presence of lone pairs, [15] and hard soft acid base theory, [2a, 16] we set out to establish whether size‐selective Ln 3+ binding sites can be engineered into coiled coils, and to obtain crucial, and currently lacking, structural information regarding Ln 3+ coiled coils.…”
Section: Figurementioning
confidence: 99%
“…We also avoided cysteine because of potential issues with redox chemistry and to reserve it for introducing function in follow-on studies. 11,19,20…”
Section: Introductionmentioning
confidence: 99%