pH/ROS dual-sensitive and chondroitin sulfate wrapped poly (β-amino ester)-SA-PAPE copolymer nanoparticles for macrophage-targeted oral therapy for ulcerative colitis

Xu, Haiting; Luo, Rui; Dong, Lingling; Pu, Xiulan; Chen, Qiyan; Ye, Naijing; Qi, Shanshan; Han, Xiaoqin; Nie, Wenbiao; Fu, Chaomei; Hu, Yichen; Zhang, Jinming; Gao, Fei

doi:10.1016/j.nano.2021.102461

Cited by 42 publications

(30 citation statements)

References 51 publications

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“…The overall protein concentration was ascertained with the use of a BCA protein assay tool in the light of the manufacturers’ instructions. Commensurable protein was resolved by 10% sodium dodecyl sulfate-polyacrylamide gels and subsequently transferred to polyvinylidene fluoride membrane ( Xu et al, 2021 ). The blots were blocked for 2 h with 5% skim milk in TBST buffer, followed by incubation throughout the night under the temperature of 4°C based on primary antibody: mouse monoclonal anti-beta-actin (1:3,000), rabbit polyclonal anti-NALP3/CIAS1(1:1,000), rabbit polyclonal anti-Caspase1 p20 (1:1,000, rabbit polyclonal anti-TLR4 (1:1,000), rabbit polyclonal anti-MyD88 (1:1,000) and rabbit polyclonal anti-NF-κB p65 (1:1,000).…”

Section: Methodsmentioning

confidence: 99%

Gastroprotective Effects of Periplaneta americana L. Extract Against Ethanol-Induced Gastric Ulcer in Mice by Suppressing Apoptosis-Related Pathways

Chen

Zhang

et al. 2021

Front. Pharmacol.

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Although Periplaneta americana L. and its modern preparation, Kangfuxin liquid, have been extensively applied for ulcerative diseases in gastrointestinal tract (e.g., gastric ulcer (GU) and ulcerative colitis, the effective components and potential mechanisms) remain unclear. In accordance with the accumulating research evidences, the relieving/exacerbating of GU is noticeably correlated to focal tissue programmed cell death. Herein, gastro-protective effects of the effective Periplaneta americana L. extract (PAE) fraction were assessed in vitro and in vivo, involving in programmed cell death-related signaling channels. To screen the effective PAE fraction exerting gastroprotective effects, several PAE fractions were gained based on a wide range of ethanol solution concentration, and they were assessed on ethanol-induced ulcer mice. Based on HPLC investigation with the use of nucleosides, the chemical composition of screened effective PAE, extracted by 20% ethanol, was analyzed in terms of quality control. Based on CCK-8 assay, the protective effects on GES-1 cells, impaired by ethanol, of PAE were assessed. After 3 days pre-treatment with PAE (200, 400, 800 mg/kg), the gastric lesions were assessed by tissue morphology, and periodic acid-schiff (PAS) staining, as well as hematoxylin and eosin (H&E) based histopathology-related investigation. The levels for inflammation cytokines (IL1-β, TNF-α, IL-18, PGE2, and IL-6), antioxidant indices (SOD and MDA) were examined via ELISA. In the meantime, based on Western Blotting assay, the expression levels of some programmed cell death-related protein targets (NLRP3, caspase-1, NF-κB p65, MyD88, and TLR4) were analyzed. As revealed from the results, PAE is capable of alleviating gastric mucosa impairment, suppressing the inflammatory cytokines, and down-regulating the MyD88/NF-κB channels. Accordingly, 20% ethanol extract of Periplaneta americana L. would contribute its gastroprotective effects, thereby providing the evidence that its anti-GU mechanisms correlated with inhibiting programmed cell death channel.

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Section: Methodsmentioning

confidence: 99%

Gastroprotective Effects of Periplaneta americana L. Extract Against Ethanol-Induced Gastric Ulcer in Mice by Suppressing Apoptosis-Related Pathways

Chen

Zhang

et al. 2021

Front. Pharmacol.

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“…Xu et al fabricated curcumin-loaded chitosan/alginate hydrogel (CA-Gel) administration strategy with dual pH and ROS sensitivity. Chondroitin sulfate, which displayed good macrophage targeting, encapsulated curcumin and combined with CA-Gel to form nanoparticles ( Xu et al, 2022 ). These oral nanoparticles released curcumin into macrophages and mitigated inflammatory responses through the TLR4-MAPK/NF-κB signaling pathway in mice with ulcerative colitis.…”

Section: Empirical Sci Treatment Involving Biomaterial-based Tlr4-nf-...mentioning

confidence: 99%

“…Remarkably, it is a promising trend that composite hydrogel scaffolds composed of several natural materials are utilized in the application of biomaterials for drug delivery. Chitosan/alginate hydrogel loaded with curcumin is one such example, which alleviates inflammation by TLR4-MAPK/NF-κB signaling pathway ( Xu et al, 2022 ). Furthermore, the micronano chitosan/chondroitin sulfate curcumin-loaded hydrogel formed by polyelectrolytic complexation is a novel kind of anti-tumor biomaterial.…”

Section: Empirical Sci Treatment Involving Biomaterial-based Tlr4-nf-...mentioning

confidence: 99%

Strategies for Biomaterial-Based Spinal Cord Injury Repair via the TLR4-NF-κB Signaling Pathway

Shen

Cheng

et al. 2022

Front. Bioeng. Biotechnol.

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The repair and motor functional recovery after spinal cord injury (SCI) has remained a clinical challenge. Injury-induced gliosis and inflammation lead to a physical barrier and an extremely inhibitory microenvironment, which in turn hinders the recovery of SCI. TLR4-NF-κB is a classic implant-related innate immunomodulation signaling pathway and part of numerous biomaterial-based treatment strategies for SCI. Numerous experimental studies have demonstrated that the regulation of TLR4-NF-κB signaling pathway plays an important role in the alleviation of inflammatory responses, the modulation of autophagy, apoptosis and ferroptosis, and the enhancement of anti-oxidative effect post-SCI. An increasing number of novel biomaterials have been fabricated as scaffolds and carriers, loaded with phytochemicals and drugs, to inhibit the progression of SCI through regulation of TLR4-NF-κB. This review summarizes the empirical strategies for the recovery after SCI through individual or composite biomaterials that mediate the TLR4-NF-κB signaling pathway.

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“…The ratio of EPP and PVA was set from 1:10 to 1:5 (w/w). The morphology of scaffolds was usually observed by TEM and SEM [19,20]. Then, we selected SEM for the detection of the morphology of PVA/EPP fibers.…”

Section: Physicochemical Characterization Of the Pva/epp Fibersmentioning

confidence: 99%

An Electrospun Scaffold Loaded with an Enteromorpha Polysaccharide for Accelerated Wound Healing in Diabetic Mice

et al. 2022

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The design and development of innovative multifunctional wound dressing materials in engineered biomaterials is essential for promoting tissue repair. In this study, nanofibrous wound dressing materials loaded with anti-inflammatory ingredients were manufactured by a promising electrospinning strategy, and their capability for treating diabetic wounds was also investigated. A scaffold blend consisting of an Enteromorpha polysaccharide and polyvinyl alcohol (PVA) was fabricated. The in vitro and in vivo studies confirmed the efficacy of PVA/EPP1 fiber. We found that PVA/EPP1 fiber accelerated the repair of a full-thickness skin wound in diabetic mice. The results suggest that this scaffold could effectively shorten the wound healing time by inhibiting inflammatory activity, which makes it a promising candidate for the treatment of hard-to-heal wounds caused by diabetes.

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pH/ROS dual-sensitive and chondroitin sulfate wrapped poly (β-amino ester)-SA-PAPE copolymer nanoparticles for macrophage-targeted oral therapy for ulcerative colitis

Cited by 42 publications

References 51 publications

Gastroprotective Effects of Periplaneta americana L. Extract Against Ethanol-Induced Gastric Ulcer in Mice by Suppressing Apoptosis-Related Pathways

Gastroprotective Effects of Periplaneta americana L. Extract Against Ethanol-Induced Gastric Ulcer in Mice by Suppressing Apoptosis-Related Pathways

Strategies for Biomaterial-Based Spinal Cord Injury Repair via the TLR4-NF-κB Signaling Pathway

An Electrospun Scaffold Loaded with an Enteromorpha Polysaccharide for Accelerated Wound Healing in Diabetic Mice

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