An Electrospun Scaffold Loaded with an Enteromorpha Polysaccharide for Accelerated Wound Healing in Diabetic Mice

Abstract

The design and development of innovative multifunctional wound dressing materials in engineered biomaterials is essential for promoting tissue repair. In this study, nanofibrous wound dressing materials loaded with anti-inflammatory ingredients were manufactured by a promising electrospinning strategy, and their capability for treating diabetic wounds was also investigated. A scaffold blend consisting of an Enteromorpha polysaccharide and polyvinyl alcohol (PVA) was fabricated. The in vitro and in vivo studies confirmed the efficacy of PVA/EPP1 fiber. We found that PVA/EPP1 fiber accelerated the repair of a full-thickness skin wound in diabetic mice. The results suggest that this scaffold could effectively shorten the wound healing time by inhibiting inflammatory activity, which makes it a promising candidate for the treatment of hard-to-heal wounds caused by diabetes.

Enhancing diabetic wound healing: advances in electrospun scaffolds from pathogenesis to therapeutic applications

“…The optimized Cu-GZS exhibited a constant copper release rate and accelerated wound closure, as evidenced by reduced leukocyte infiltration, intact epithelium with normal keratinization, and faster wound size reduction. Guo et al (2022) developed a nanofibrous scaffold composed of marshmallow polysaccharide (EPP1) and polyvinyl alcohol (PVA), which exhibited excellent water absorption and anti-inflammatory activity. In vitro and in vivo experiments demonstrated that PVA/EPP1 fiber scaffolds accelerated wound repair in diabetic mice.…”

Electrospinning Nanofibers as a Dressing to Treat Diabetic Wounds

“…The dressing inhibits the inflammatory response while accelerating the healing process. While remaining biocompatible, the dressing achieved a high water absorption capacity, demonstrating its potential for use as a diabetic wound dressing [82].…”

Radially Electrospun Fibrous Membrane Incorporated with Copper Peroxide Nanodots Capable of Self-Catalyzed Chemodynamic Therapy for Angiogenesis and Healing Acceleration of Diabetic Wounds

“…Many strategies including gas therapy, growth factors, and functional nanoparticles have been introduced to accelerate diabetic wound healing. , Among them, inorganic nanomaterials containing zinc (Zn 2+ ), copper (Cu 2+ ), and cobalt (Co 2+ ) ions have been widely applied in the treatment of diabetic wounds due to their inherent anti-infection abilities. − In particular, these metal ions are involved in the secretion of angiogenic-related cytokines, showing capacities to endogenously mitigate the lack of blood supply. − More recently, metal peroxides such as CaO 2 , MgO 2 , and CuO 2 nanoparticles have been considered promising to accelerate diabetic wound healing because they can store H 2 O 2 in solid form but simultaneously release H 2 O 2 molecules and metal ions once triggered by acidic pH. − As known, H 2 O 2 is a commonly used disinfectant to treat infected wounds, while the metal ions including Ca 2+ , Mg 2+ , and Cu 2+ could display multiple physiological functions favorable to wound healing . Zeng et al presented a microneedle dressing modified with polydopamine (PDA) coated CaO 2 nanoparticles (CaO 2 @PDA), which not only exhibited good antibacterial effects synergistically activated by the photothermal conversion of PDA under NIR irradiation and H 2 O 2 generated from CaO 2 in response to acidic environment but also inhibited inflammation and accelerated wound healing in diabetic rats .…”

“…Many strategies including gas therapy, growth factors, and functional nanoparticles have been introduced to accelerate diabetic wound healing. 4,5 Among them, inorganic nanomaterials containing zinc (Zn 2+ ), copper (Cu 2+ ), and cobalt (Co 2+ ) ions have been widely applied in the treatment of diabetic wounds due to their inherent anti-infection abilities. 6−10 In particular, these metal ions are involved in the secretion of angiogenic-related cytokines, showing capacities to endogenously mitigate the lack of blood supply.…”