Pharmacological basis for the medicinal use of Psidium guajava leave in hyperactive gut disorders

2015

In vitro study was carried out to explore the pharmacological basis of crude extract of Buxus wallichiana for its folkloric uses in gastrointestinal, respiratory and vascular disorders. In jejunum preparations, crude extract (0.03 ± 1.0 mg/mL) caused a transient spasmogenic effect followed by the spasmolytic effect at higher doses (3.0–10 mg/mL). In atropinized jejunum preparation, crude extract inhibited the spontaneous and K+ (80 mM)-induced contraction, suggesting that spasmolytic effect is mediated through the Ca+2-channel blockade. The Ca+2-channel blockade effect was confirmed when pretreatment of tissue with extract produced a dose-dependent shift in Ca+2 concentration-response curves to the right, similarly as verapamil. Furthermore, crude extract exhibited non-specific relaxant effect on carbachol- (1 µM) and K+ (80 mM)-induced tracheal contractions, suggesting the coexistence of anticholi-nergic and Ca+2-antagonistic properties. Moreover, it relaxed the K+ (80 mM)- and phenylephrine (1 µM)-induced contraction in rabbit aorta, suggesting the Ca+2-channel blockade. These findings may validate the folkloric uses of B. wallichiana in constipation, bronchitis, asthma and hypertension.

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Section: Discussionmentioning

confidence: 99%

Pharmacological basis for the folkloric uses of Buxus wallichiana in gastrointestinal, respiratory and vascular disorders

Hussain

Raza

2015

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“…It has been observed that most of the medicinal plant and plant-derived test compounds exhibit inhibitory effect through K + channel activation (Gilani et al, 2008;Mehmood et al, 2015;Quast, 1992) or Ca ++ channel blockade like mechanisms (Shah et al, 2011;Janbaz et al, 2014). Challenge of isolated tissues with low K + (25 mM) and high K + (80 mM) induces depolarization which is usually practiced Table I Antidiarrheal effect of the crude extract of P. bistorta against castor oil-induced diarrhea in mice Chi-square-test) to distinguish K + channel opening and Ca ++ channel blocking like activities .…”

Section: Resultsmentioning

confidence: 99%

“…On account of medicinal use of P. bistorta in hyperactive gut disorders (Duke et al, 2002), it was first evaluated for its antidiarrheal activity against castor oil-induced diarrheal model in mice. Further to explore the scientific basis for its observed antidiarrheal activity, the test material was subjected studied for its spasmolytic effect on isolated tissues of rabbit jejunum and guinea pig ileum (Mehmood and Gilani, 2010;Shah et al, 2011;Janbaz et al, 2014;2015a).…”

Section: Resultsmentioning

confidence: 99%

Antidiarrheal and antispasmodic activities of Polygonum bistorta rhizomes are mediated predominantly through K+ channels activation

Ali

Mehmood

et al. 2015

Polygonum bistorta is a popular medicinal herb used to treat diarrhea. This study provides pharmacological basis to its folk use in diarrhea using in vivo and in vitro assays. Administration of P. bistorta rhizomes extract to mice offered protection against castor oil-induced diarrhea at 300-1,000 mg/kg and was found safe up to the dose of 5 g/kg. In isolated rabbit jejunum, the extract caused a dose-dependent relaxation of spontaneous and low K+ (25 mM)-induced contractions with weak effect against high K+ (80 mM). In tissues pretreated with glibenclamide or tetraethylammonium chloride (TEA), the relaxant effect of the extract was markedly inhibited by TEA only. While verapamil showed complete relaxation of spontaneous, low K+, low K+ with TEA and high K+-induced contractions. In guinea-pig ileum, mild atropine-sensitive effect was observed. This study indicates that P. bistorta possesses anti-diarrheal and antispasmodic activities mediated predominantly through K+-channels activation along with weak Ca++ antagonist effect.

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“…It has been observed that most of the medicinal plants or plant based remedies exhibit inhibitory effect on spontaneously contracting rabbit jejunum through K + channel activation (Gilani et al, 2008;Khan et al, 2011) and/or Ca ++ channel blockade like mechanisms (Shah et al, 2011;Khan et al, 2013;Janbaz et al, 2014). To study the involvement of these inhibitory pathways, low K + (25 mM) and high K + (80 mM) are used to depolarize tissues which distinguish K + channel opening (KCO) and Ca ++ antagonist like mechanisms (Gilani et al, 2008;Janbaz et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Antidiarrheal and antispasmodic activities of Adiantum capillus-veneris are predominantly mediated through ATP-dependent K+ channels activation

Hassan

Mehmood

et al. 2015