Pharmacological/Biological Effects of Berberine

Buerba, Eva Ma Gálvez; Pérez-Hernández, Marta; Domingo, María Pilar; Núñez-Jurado, David; Cebolla, Vicente L.; Matt, Muriel; Pardo, Julián

doi:10.1007/978-3-642-22144-6_182

Cited by 8 publications

(4 citation statements)

References 160 publications

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“…It is commonly used for the clinical treatment of gastroenteritis, bacterial diarrhoea, intestinal infections and conjunctivitis. 14 The MTT assay is a commonly used method to estimate the metabolic activity of living cells to detect proliferation, differentiation and cell metabolism. This test is based on the reduction of MTT by succinate dehydrogenase in living cells to form blue crystals, which can be quantified spectrophotometrically.…”

Section: Discussionmentioning

confidence: 99%

Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells

Zhu

et al. 2020

Innate Immun

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Berberine is an alkaloid extracted from medicinal plants such as Coptis chinensis and Phellodendron chinense. It possesses anti-inflammatory, anti-tumour and anti-oxidation properties, and regulates Glc and lipid metabolism. This study explored the mechanisms of the protective effects of berberine on barrier function and inflammatory damage in porcine intestinal epithelial cells (IPEC-J2) induced by LPS. We first evaluated the effects of berberine and LPS on cell viability. IPEC-J2 cells were treated with 5 μg/ml LPS for 1 h to establish an inflammatory model, and 75, 150 and 250 μg/ml berberine were used in further experiments. The expression of IL-1β, IL-6 and TNF-α was measured by RT-PCR. The key proteins of the NF-κB/MAPK signalling pathway (IκBα, p-IκBα, p65, p-p65, c-Jun N-terminal kinase (JNK), p-JNK, p38, p-p38, ERK1/2 and p-ERK1/2) were detected by Western blot. Upon exposure to LPS, IL-1β, IL-6 and TNF-α mRNA levels and p-IκBα p-p65 protein levels were significantly enhanced. Pre-treatment with berberine reduced the expression of inflammatory factors and was positively correlated with its concentration, and dose dependently inhibited the expression of IκBα, p-IκBα, p-p65, p-p38 and JNK. These results demonstrated that pre-treating intestinal epithelial cells with berberine was useful in preventing and treating diarrhoea induced by Escherichia coli in weaned pigs.

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Section: Discussionmentioning

confidence: 99%

Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells

Zhu

et al. 2020

Innate Immun

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“…It is present in several medicinal plants, such as Berberis vulgaris, Coptis chinensis, Hydrastis canadensis, Coptidis rhizoma, Xanthoriza simplicissima, Phellodendron amurense, and Chelidonium majus. Berberine exhibits unusual biochemical and pharmacological activities, including antidiabetic [1], hypolipidemic [2], antihypertensive [3], anti-inflammatory [4], antidiarrheal [5], hepatoprotective [6], antidepressant [7], anticancer [8], antibacterial [9], and antiviral [10] properties. BBR is capable of penetrating all cell lines, but the cumulative concentration is the highest in Hep G-2 cells [11].…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity of berberine

2020

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Plants are a rich source of new antiviral, pharmacologically active agents. The naturally occurring plant alkaloid berberine (BBR) is one of the phytochemicals with a broad range of biological activity, including anticancer, anti-inflammatory and antiviral activity. BBR targets different steps in the viral life cycle and is thus a good candidate for use in novel antiviral drugs and therapies. It has been shown that BBR reduces virus replication and targets specific interactions between the virus and its host. BBR intercalates into DNA and inhibits DNA synthesis and reverse transcriptase activity. It inhibits replication of herpes simplex virus (HSV), human cytomegalovirus (HCMV), human papillomavirus (HPV), and human immunodeficiency virus (HIV). This isoquinoline alkaloid has the ability to regulate the MEK-ERK, AMPK/mTOR, and NF-κB signaling pathways, which are necessary for viral replication. Furthermore, it has been reported that BBR supports the host immune response, thus leading to viral clearance. In this short review, we focus on the most recent studies on the antiviral properties of berberine and its derivatives, which might be promising agents to be considered in future studies in the fight against the current pandemic SARS-CoV-2, the virus that causes COVID-19.

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“…Berberine is an isoquinoline alkaloid found in members of the plant families such as Menispermaceae and Berberidaceae. The alkaloid exhibits several pharmacological and biological effects (Galvez et al, 2013). Berberine is accumulated in leaves, root, stem, lowers and fruit.…”

Section: Introductionmentioning

confidence: 99%

In vitro synthesis of berberine, a pharmacologically important isoquinoline alkaloid in callus cultures of Tinospora cordifolia

Adithya¹,

Narasimhan²

2020

ijrps

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Callus cultures were raised from young green leaf segments of the medicinal climber Tinospora cordifolia in Murashige and Skoog’s media containing different growth regulator regime. The in vitro callus cultures were analysed for the accumulation of active principle through high-performance liquid chromatography. Quantitative analysis of berberine was achieved by thin-layer chromatography and UV-absorbance method. Callus cultures accumulated an appreciable amount of berberine. A maximum of 355 μg/g dry wt accumulation of berberine was noted when the callus was grown in the presence of 2 mg/l 2, 4-D and 0.1 mg/l BA. However, maximum callus growth was achieved in the presence of 0.5 mg/l BA and 2 mg/l 2,4-D. Maximum tissue growth didn't favour an increased accumulation of the berberine. Enhanced accumulation of berberine was found when callus growth was less and therefore may be associated with the stress faced by in vitro tissues. The experimental results indicate that it is desirable to adopt two different growth regulator regime - one for accelerated callus growth and another for enhanced in vitro synthesis of berberine. The in vitro cultures of T. cordfiolia also offers an ideal system for understanding the studies on elucidating alternate routes of berberine biosynthetic pathway as well as possible elite cells for scaling up of berberine.

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Pharmacological/Biological Effects of Berberine

Cited by 8 publications

References 160 publications

Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells

Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells

Antiviral activity of berberine

In vitro synthesis of berberine, a pharmacologically important isoquinoline alkaloid in callus cultures of Tinospora cordifolia

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