Pharmacological evaluation of <i>Verbesina encelioides</i>

Jain, S. C.; Purohit, M. C.; Sharma, Rajan

doi:10.1002/ptr.2650020309

Cited by 11 publications

(6 citation statements)

References 9 publications

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“…Verbesina encelioides (VE) , member of Asteraceae (Sunflower) family, is native to the United States, Mexican Plateau, Europe, and Asia including Saudi Arabia [ 19 ]. It is a notorious weed and an ornamental plant with various bio efficacies like antibacterial, antifungal, antiviral, hypoglycemic and implantation activities [ 20 ]. Traditionally V. encelioides finds use for the treatment of sore gums and hemorrhoids [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Verbesina encelioides: cytotoxicity, cell cycle arrest, and oxidative DNA damage in human liver cancer (HepG2) cell line

Al-Oqail

Siddiqui

Al-Sheddi

et al. 2016

BMC Complement Altern Med

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BackgroundCancer is a major health problem and exploiting natural products have been one of the most successful methods to combat this disease. Verbesina encelioides is a notorious weed with various pharmacological properties.The aim of the present investigation was to screen the anticancer potential of V. encelioides extract against human lung cancer (A-549), breast cancer (MCF-7), and liver cancer (HepG2) cell lines.MethodsA-549, MCF-7, and HepG2 cells were exposed to various concentrations of (10–1000 μg/ml) of V. encelioides for 24 h. Further, cytotoxic concentrations (250, 500, and 1000 μg/ml) of V. encelioides induced oxidative stress (GSH and LPO), reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP), cell cycle arrest, and DNA damage in HepG2 cells were studied.ResultsThe exposure of cells to 10–1000 μg/ml of extract for 24 h, revealed the concentrations 250–1000 μg/ml was cytotoxic against MCF-7 and HepG2 cells, but not against A-549 cells. Moreover, the extract showed higher decrease in the cell viability against HepG2 cells than MCF-7 cells. Therefore, HepG2 cells were selected for further studies viz. oxidative stress (GSH and LPO), reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP), cell cycle arrest, and DNA damage. The results revealed differential anticancer activity of V. encelioides against A-549, MCF-7 and HepG2 cells. A significant induction of oxidative stress, ROS generation, and MMP levels was observed in HepG2 cells. The cell cycle analysis and comet assay showed that V. encelioides significantly induced G2/M arrests and DNA damage.ConclusionThese results indicate that V. encelioides possess substantial cytotoxic potential and may warrant further investigation to develop potential anticancer agent.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1106-0) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Verbesina encelioides: cytotoxicity, cell cycle arrest, and oxidative DNA damage in human liver cancer (HepG2) cell line

Al-Oqail

Siddiqui

Al-Sheddi

et al. 2016

BMC Complement Altern Med

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“…Isoprenoids, in particular eudesmane sesquiterpenes, are reportedly the characteristic constituents of this genus . Antifungal, antibacterial, and antiviral activity has been described for Verbesina encelioides , but the compounds responsible for the activity were not identified . On the other hand, several eudesmane sesquiterpenes that have been isolated from other plants, mostly Asteraceae, have shown antifungal and antibacterial activity against human pathogens. − However, their efficacy against plant pathogens has not been previously reported.…”

Section: Resultsmentioning

confidence: 99%

Eudesmane Sesquiterpenes from Verbesina lanata with Inhibitory Activity against Grapevine Downy Mildew

et al. 2017

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An in-house library of more than 3000 extracts of plant and fungal origin was screened against some major plant pathogens. As one of the hits, an ethyl acetate extract from inflorescences of Verbesina lanata showed significant inhibitory activity in vitro against grapevine downy mildew (Plasmopara viticola), with a MIC value of 35 μg/mL. An emulsifiable concentrate formulation with 50 mg/g of the extract was developed for in vivo evaluation. A suspension of the formulation containing 1 mg/mL of extract lowered leaf surface infection of grapevine seedling by 82% compared to the nontreated control. With the aid of HPLC-based activity profiling, the antifungal activity was correlated with a series of lipophilic compounds. Preparative isolation by a combination of chromatographic techniques afforded 16 eudesmane sesquiterpenes including eight new congeners. Nine compounds were obtained in sufficient quantities to be tested in vitro and were found to inhibit the zoospore activity of P. viticola with MIC values ranging from 4 to 50 μg/mL. The two major compounds, 6β-cinnamoyloxy-4β,9β,15-trihydroxyeudesmane (9) and 6β-cinnamoyloxy-1β,15-dihydroxyeudesm-4-en-3-one (13), showed MIC values of 5 and 31 μg/mL, respectively.

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“…These results are consistent with those obtained by other investigators who found an antimicrobial activity of some oils against many of pathogens in vitro (Madhukor and Reddy, 1989 ;Farag et al,1989;Deans et al, 1992;Mc Cutcheon et al, 1994 andNavarro et al,1996). Some essential oils have an allelopathic effect on some diseases on other plant hosts as previous investigators have reported (Jain, et al,1992;Paran et al,1996;El-Shoraky 1998 andGouda, 2001). El-Kazzaz et al, 2003 studied the effect of essential oil, Mint, Clove, Cumin and Basil on Sclerotium rolfsii Sacc., the causal pathogen of sugar beet damping-off and root rot diseae .…”

Section: Discussionmentioning

confidence: 75%

“…Sclerotium rolfsii and Rhizoctonia solani were considered among the most destructive diseases affecting yield crop in Egypt (El-Abyad et al, 1992;El-Kazzaz et al,1999 andEl-Kholi 2000 ;Eah 2000 andGouda 2001). Some essential oils have an allelopathic effect on some diseases on other plant hosts as previous investigators have reported (Jain,et al,1992;Paran et al,1996;Elshoraky 1998 andGouda, 2001).…”

Section: Introductionmentioning

confidence: 76%

Effect of Some Essential Oils on Controlling Sugar Beet Damping - Off and Root Rot Diseases Caused by Many Serious Soil Borne Fungal Pathogens in Nile Delta Region .

Gouda

El-Fahar

El-Shoraky

2009

Journal of Plant Protection and Pathology

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Testing the effect of some essenstial oils on controlling of some causal pathogens of sugar beet plants in Nile Delta soil,( Sclerotium rolfsii Sacc.; Rhizoctonia solani Khun.; Fusarium oxysporum Schlech and Macrophomina phaseolina) (Tassi) Goid. In vitro, oils of Syzygium aromaticum L. (clove), Cuminum cyminum L. (cumin), Ocimum basilicum L.(basil) Mentha viridis (mint) were effective as inhibitors of the linear growth of S. rolfsii R. solani, M. Phaseolina at the concentration of 1500 ppm. On the other hand, they were affected slightly the linear growth and sporulation of F. Oxysporum at the concentration of 2000 ppm. At the same time, increasing the concentration of the tested materials significantly reduced the linear growth of all the tested pathogens.Under greenhouse and field conditions, data show that oils have significant effect in improving the number of survived seedlings and reduce of root rot. Oils of Syzygium aromaticum L. (Clove) and Cuminum syminum L. (Cumin) were suprior than the other materials in reducing infection with pre-and post damping -off and root rots of sugar beet as well as the disease severity. The rest oils were less effective in decreasing the disease incidence. Oils improved morpholigical characters expressed as plant height, Leaf area and dry weight per plant, also increasing the yield component, total soluble sugars (TSS), sucrose percent in root and sugar purity.The fungicide Rizolex T. 50 was used for comparative studies in controlling these diseases.

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Pharmacological evaluation of Verbesina encelioides

Abstract: Various fractions of Verbesina encelioides have demonstrated significant antibacterial, antifungal, antiviral, antitumor, hypoglycaemic and anti-implantation activities.

Cited by 11 publications

References 9 publications

Verbesina encelioides: cytotoxicity, cell cycle arrest, and oxidative DNA damage in human liver cancer (HepG2) cell line

Verbesina encelioides: cytotoxicity, cell cycle arrest, and oxidative DNA damage in human liver cancer (HepG2) cell line

Eudesmane Sesquiterpenes from Verbesina lanata with Inhibitory Activity against Grapevine Downy Mildew

Effect of Some Essential Oils on Controlling Sugar Beet Damping - Off and Root Rot Diseases Caused by Many Serious Soil Borne Fungal Pathogens in Nile Delta Region .

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