Phenotypic and genotypic features of Aggregatibacter actinomycetemcomitans isolated from patients with periodontal disease

Wahasugui, Thais C.; Nakano, Viviane; Piazza, Roxane M. F.; Avila-Campos, Mario Júlio

doi:10.1016/j.diagmicrobio.2012.12.013

Cited by 12 publications

(10 citation statements)

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“…Additionally, we related the virulence factor genes expressions from different A. actinomycetemcomitans to an early development of periodontal disease. The allotment patterns of A. actinomycetemcomitans serotypes were assessed by numerous studies and by different experimental designs [13,43,44,47,48]. Studies have suggested that highly pathogenic strains usually belong to serotype b; therefore, there may be an association between serotype b and aggressive periodontitis [15, 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

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Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions

et al. 2018

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Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are strongly associated with periodontitis, and their evaluations are relevant to understand their role in the etiology and progression of periodontal diseases. In this study, the qualitative and quantitative detection of A. actinomycetemcomitans and F. nucleatum, as well as their genetic diversity, were evaluated in individuals with gingivitis, chronic periodontitis and periodontally healthy. In addition, the biotyping, serotyping, and prevalence of the ltx and cdt genes in A. actinomycetemcomitans were also determined. Subgingival biofilms obtained from gingivitis (70), periodontitis (75) and healthy (95) individuals were analyzed by cultures and PCR. Bacterial typing and presence of ltx and cdt genes in A. actinomycetemcomitans were also verified. DNA from A. actinomycetemcomitans and F. nucleatum was detected respectively, in 65.7% and 57.1% of gingivitis, 80% and 68% of periodontitis, and 57.8% and 37.8% of healthy. A. actinomycetemcomitans from gingivitis were biotypes I, II, IV, V, and X, and serotypes a, c, and e. In periodontitis, biotypes II, VI, and X, and serotypes a, b, and c were found. In healthy subjects, biotypes II and X, and serotypes b and c were found. The LTX and ltxA were observed in strains from gingivitis and periodontitis pockets. Subsequently, our data also showed no direct relationship between ltxA gene expression and leukotoxin gene 530-bp presence. On the other hand, cdt gene predominated during the inflammatory disease process. Our results strongly support a role of A. actinomycetemcomitans and F. nucleatum in advanced stage of periodontal disease.

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Section: Discussionmentioning

confidence: 99%

“…Wahasugui et al [ 43 ] reported the presence of 64 A. actinomycetemcomitans strains as highly leukotoxic and only six strains as minimally leukotoxic. In addition, the presence of these leukotoxic strains had not association with the development of periodontitis, suggesting its transitory stage in oral cavity.…”

Section: Discussionmentioning

confidence: 99%

Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions

et al. 2018

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“…En este estudio, fue observado que la mayoría de los pacientes albergó un único biotipo, siendo el más prevalente el biotipo II (Figura 2). En el Brasil, Avila-Campos et al 19 y Wahagusi et al 22 , relataron la predominancia de cepas del biotipo X. Esas diferencias observadas, muestran la diversidad y heterogeneidad de este grupo microbiano. Por otro lado, en el Brasil existe una población multiétnica, con fuerte mezcla de razas y hábitos peculiares y la ocurrencia de diferentes patógenos bucales en biopelícula dental o subgingival puede haber influenciado en estos resultados.…”

Section: Discussionunclassified

“…Análisis estadístico. Los valores obtenidos en este estudio, fueron analizados por el Índice de Kappa (K) 22 para verificación de la concordancia entre la detección bacteriana por los métodos de cultivo y PCR, así como también, para demostrar la especificidad y sensibilidad de los ensayos utilizados en la detección de los organismos evaluados. K= índice kappa; P < 0,005 = K ≠ 0 *Valores estadísticamente significativos se puede notar que las muestras negativas por cultivo, fueron positivas para la detección bacteriana por PCR, y los partidores ASH y FN-5047 mostraron valores estadísticamente significativos.…”

Section: Métodosunclassified

Aggregatibacter actinomycetemcomitans y Fusobacterium nucleatum en biopelículas subgingivales de pacientes brasileños con y sin enfermedad periodontal: comparación de dos métodos de detección

Malheiros

Ávila-Campos

2018

Odontol. sanmarquina

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Objetivo: Realizar la detección comparativa de cepas de A. actinomycetemcomitans y F. nucleatum de muestras subgingivales por los métodos de cultivo y de reacción en cadena de la polimerasa (PCR). Métodos: Fueron evaluados 50 pacientes con periodontitis crónica (P) y 50 pacientes sanos (S). Las muestras fueron colectadas de bolsas periodontales y surcos gingivales. El cultivo bacteriano fue realizado en agar tripticasa de soya-suero de caballo-bacitracina-vancomicina, e incubado en anaerobiosis. La identificación bacteriana fue por métodos bioquímicos de fermentación de carbohidratos y por PCR. Resultados: Por el método de cultivo, de las 50 muestras de periodontitis, 9 (18%) fueron positivas para A. actinomycetemcomitans aislándose 17 cepas. También, de esas muestras, 10 (20%) fueron positivas para F. nucleatum aislándose 19 cepas. De las 50 muestras de pacientes sanos, solamente 1 (2%) fue positiva para A. actinomycetemcomitans obteniéndose 2 cepas, y 12 (24%) positivas para F. nucleatum con 18 cepas. Por PCR fueron observadas diferencias en la detección de A. actinomycetemcomitans, entre los tres pares de partidores utilizados, para muestras de bolsa periodontal y surco gingival: partidor AA, 96% y 86%; partidor FU, 48% y 42%; y partidor ASH, 24% y 6%. Los porcentajes de detección para F. nucleatum de muestras de P y S fueron: partidor FN-5047, 36% y 18%; y partidor 505-S, 8% para ambas muestras colectadas. Cepas de A. actinomycetemcomitans biotipo II fueron las más prevalentes. Conclusiones: EL método de PCR fue más sensible y específico en la detección bacteriana que el cultivo.

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“…18 (2007) suggested that highly leukotoxic A. actinomycetemcomitans strains comprise a single clone. But, regarding minimally leukotoxic strains, Wahasugui, et al 32 (2013) found that such strains belonged to 8 different biotypes.…”

Section: Discussionmentioning

confidence: 99%

Leukotoxicity of Aggregatibacter actinomycetemcomitans in generalized aggressive periodontitis in Brazilians and their family members

Silveira

Nogueira

Nogueira³

et al. 2013

J. Appl. Oral Sci.

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ObjectiveThe purpose of this study was to examine the leukotoxin promoter types of Aggregatibacter actinomycetemcomitans clones in subjects with generalized aggressive periodontitis (GAgP) and in their family members (FM). Material and MethodsThirty-five patients with GAgP (33.9±7.1 years), 33 of their FM (22.8±11.4 years), and 41 patients with chronic periodontitis (CP) (44.1±9.4 years) were clinically analyzed using the plaque index, gingival index, probing depth (PD), and clinical attachment level (CAL). Subgingival biofilm samples were collected from four interproximal periodontal sites (>PD and >CAL) of each patient. The presence of A. actinomycetemcomitans and its leukotoxic clone was confirmed by polymerase chain reaction (PCR). ResultsA. actinomycetemcomitans was observed in 23 (51.1%) GAgP patients and 16 (30.1%) CP patients. Thirty-seven (94.8%) patients showed minimally leukotoxic strains and 2 (5.1%) showed highly leukotoxic strains. In the FM group, 10 (30.3%) had aggressive periodontitis (AgP), 12 (36.3%) had CP, 11 (33.3%) were periodontally healthy or had gingivitis, and 12.2% were A. actinomycetemcomitans positive. Greater full mouth PD and CAL were observed in GAgP patients positive for the bacteria than those negative for it (p<;0.05), and the presence of A. actinomycetemcomitans positively correlated with GAgP (Odds ratio, 3.1; confidence interval, 1.4-7.0; p=0.009).Conclusions The presence of A. actinomycetemcomitans was associated with the clinical condition of GAgP, with most patients exhibiting a generalized form of the disease and minimally leukotoxic clones. Most of the relatives of GAgP patients presented either CP or AgP.

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Phenotypic and genotypic features of Aggregatibacter actinomycetemcomitans isolated from patients with periodontal disease

Cited by 12 publications

References 45 publications

Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions

Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions

Aggregatibacter actinomycetemcomitans y Fusobacterium nucleatum en biopelículas subgingivales de pacientes brasileños con y sin enfermedad periodontal: comparación de dos métodos de detección

Leukotoxicity of Aggregatibacter actinomycetemcomitans in generalized aggressive periodontitis in Brazilians and their family members

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