Phenotypic variations of muscle fibre and intramuscular fat traits in Longissimus muscle of F2 population Duroc×Berlin Miniature Pig and relationships to meat quality

Fiedler, I.; Nürnberg, Karin; Hardge, T.; Nürnberg, Gerd; Ender, K.

doi:10.1016/s0309-1740(02)00075-x

Cited by 47 publications

(30 citation statements)

References 30 publications

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“…The same tendency of muscle fibre diameter in MLLT was reported by Lengerken et al (1994), who observed nearly the same white muscle fibre diameter (119.99 µm), but the diameter of intermediate and red muscle fibres exceeded our results. Fiedler et al (2003) observed the white muscle fibre diameter 61.5 µm, intermediate muscle fibre diameter 49.1 µm and red muscle fibre diameter 51.7 µm, with a considerable difference in the white muscle fibre diameter when compared with our results. Ruusunen et al (2004) reported a higher white muscle fibre diameter (85.86 µm), but a lower intermediate and red muscle fibre diameter in MLLT (61.28 µm and 59.17 µm, respectively).…”

Section: Histological Structure Of Mlltsupporting

confidence: 66%

Histological structure of the musculus longissimus lumborum et thoracis in pigs with the same ryanodine receptor genotype (CC) in relation to carcass indicators

Elias¹,

Hluchý²,

Mlynek³

2007

CZECH J ANIM SCI

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ABSTRACT:The aim of this study was to investigate the histological structure of the longissimus muscle in pigs in relation to carcass value indicators. A total number of 16 pigs of about 101.28 kg average live weight were used. The animals were raised at a Fattening and Carcass Value Experimental Station (FCVES) of Slovak University of Agriculture in Nitra in equal conditions, receiving a standard diet fortified with vitamin-mineral mixture, and they were slaughtered in an experimental abattoir of FCVES. Samples from the musculus longissimus lumborum et thoracisi (MLLT) for histological evaluation were taken within 30 minutes post mortem, immediately frozen in liquid nitrogen and stored at a temperature of -20°C. In the experimental abattoir of FCVES feeding indicators, and indicators of meat quality and carcass value were examined. Samples were processed histochemically and single types of muscle fibres were differentiated according to reactions on SDH on the basis of Vacek's (1974) method. Nikon microscopic system, Pixelink digital camera and LUCIA software for image analyses for the morphometric analysis of MLLT structure were used. The highest abundance of white and the lowest abundance of intermediate muscle fibres was obtained in the analyzed musculus longissimus lumborum et thoracisi of pigs. Red muscle fibre abundance was only slightly higher than intermediate muscle fibre abundance. Concerning the average muscle fibre diameter, the highest values in white and the lowest values in red muscle fibres were found. Positive correlations of white muscle fibre abundance with loin meat weight, thigh meat weight, carcass length, ribcase length, hot right half weight, valuable meatiness parts in kilograms, thigh weight, thigh percent in the half-carcass and MLLT area weight were obtained. In the case of all fat content and weight indicators negative correlations were obtained except loin fat weight. Red muscle fibre content showed positive correlations with shoulder fat weight, neck meat weight, neck fat weight, head weight, thigh fat weight, average backfat thickness and MLLT area. Correlation coefficients between white muscle fibre diameter and shoulder meat weight, thigh meat weight, carcass length, ribcase length showed weak positive correlations. An increase in the white muscle fibre diameter corresponds with an increase in loin meat weight, valuable meatiness parts in kg, valuable meatiness parts in percents, thigh weight, thigh percent in the half-carcass weight and MLLT area. Concerning the red muscle fibre diameter weak positive correlations were obtained in relation to neck meat weight, thigh meat weight, thigh weight and moderate positive correlations to shoulder meat weight, loin meat weight, valuable meatiness parts in kilograms and percents, thigh percent in half-carcass and MLLT area.

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Section: Histological Structure Of Mlltsupporting

confidence: 66%

Histological structure of the musculus longissimus lumborum et thoracis in pigs with the same ryanodine receptor genotype (CC) in relation to carcass indicators

Elias¹,

Hluchý²,

Mlynek³

2007

CZECH J ANIM SCI

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“…Here, the main type of muscle fiber in m. biceps femoris was different from that in m. ipsilateral pectoralis (Belichenko et al, 2004). The presence of fast-twitch glycolytic fibers, rich in chicken m. ipsilateral pectoralis, was positively correlated with SFAs such as PA and SA, whereas slow-twitch oxidative fibers abundant in chicken m. biceps femoris were positively correlated with unsaturated FAs (Fiedler et al, 2003). Caveolin-1, FATP-1 and mFABP mRNA expression levels between breeds and anatomical locations were not different.…”

Section: Discussionmentioning

confidence: 79%

Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers

Guo

Shu

Zhou

et al. 2013

Animal

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Fatty acid translocase (FAT/CD36) is a membrane receptor that facilitates long-chain fatty acid uptake. To investigate its role in the regulation of long-chain fatty acid composition in muscle tissue, we studied and compared FAT/CD36 gene expression in muscle tissues of commercial broiler chickens and Chinese local Silky fowls. The results from gas chromatography-mass spectrometry analysis of muscle samples demonstrated that Chinese local Silky fowls had significantly higher (P , 0.05) proportions of linoleic acid (LA) and palmitic acid, lower proportions (P , 0.05) of arachidonic acid (AA) and oleic acid than the commercial broiler chickens. The mRNA expression levels of fatty acid (FA) transporters (FA transport protein-1, membrane FA-binding protein, FAT/CD36 and caveolin-1) in the m. ipsilateral pectoralis and biceps femoris were analyzed by Q-PCR, and FAT/CD36 expression levels showed significant differences between these types of chickens (P , 0.01). Interestingly, the levels of FAT/CD36 expression are positively correlated with LA content (r 5 0.567, P , 0.01) but negatively correlated with palmitic acid content (r 5 20.568, P , 0.01). Further experiments in the stably transfected Chinese hamster oocytes cells with chicken FAT/CD36 cDNA demonstrated that overexpression of FAT/CD36 improves total FA uptake with a significant increase in the proportion of LA and AA, and a decreased proportion of palmitic acid. These results suggest that chicken FAT/CD36 may selectively transport LA and AA, which may lead to the higher LA deposition in muscle tissue.

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“…Like the JAZF1 gene, many genes associated with fat metabolism were applied to pig breeding as candidate genes to improve meat quality. Pork meat quality is an important trait for breeding in the porcine industry (Fiedler et al, 2003). One of the most important parameters affecting meat quality is intramuscular fat (IMF) content, which is a key determinant of organoleptic evaluation and the nutritional value of pork (Calkins and Hodgen, 2007;Oury et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and tissue expression profile analysis of the porcine JAZF1 gene

Yang¹,

He²,

Xu³

et al. 2015

Genet. Mol. Res.

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ABSTRACT.A recent study indicated that the JAZF1 gene was related to lipid metabolism by regulating the level of gene expression in humans and mice. In order to investigate whether JAZF1 gene expression was associated with fat deposition in pig, we cloned the full-coding region of the JAZF1 gene (GenBank accession No. KF307636) from porcine longissimus dorsi. Results showed that the open reading frame of JAZF1 covered 732 bp and encoded 243 amino acids. Multiple alignment of isoform sequences revealed that the deduced amino acid sequence of JAZF1 had a high degree of sequence similarity to other vertebrates, indicating that it was highly conserved during evolution. Bioinformatic analysis indicated that pig JAZF1 contained 23 phosphorylation sites and 19 glycosyl sites. JAZF1 was predicted to have 3 ZnF-C 2 H 2 and 2 low-complexity domains. The JAZF1 mRNA expression pattern indicated that JAZF1 mRNA expression level in the liver was significantly different in 2 divergent breeds (P < 0.05). This article perhaps provided an important experimental basis for further research on the mechanisms of lipid metabolism and fat deposition in pigs.

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Phenotypic variations of muscle fibre and intramuscular fat traits in Longissimus muscle of F2 population Duroc×Berlin Miniature Pig and relationships to meat quality

Cited by 47 publications

References 30 publications

Histological structure of the musculus longissimus lumborum et thoracis in pigs with the same ryanodine receptor genotype (CC) in relation to carcass indicators

Histological structure of the musculus longissimus lumborum et thoracis in pigs with the same ryanodine receptor genotype (CC) in relation to carcass indicators

Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers

Molecular characterization and tissue expression profile analysis of the porcine JAZF1 gene

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