1997
DOI: 10.1046/j.1365-2958.1997.3311709.x
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Pheromone‐inducible expression of an aggregation protein in Enterococcus faecalis requires interaction of a plasmid‐encoded RNA with components of the ribosome

Abstract: SummaryTransfer of the conjugative plasmid pCF10 from Enterococcus faecalis donor strains is induced by a peptide pheromone (cCF10) secreted by recipient cells. Highefficiency transfer requires expression of an aggregation protein (Asc10) encoded by the prgB gene and positively regulated by genes in a region 3-5 kb upstream, containing prgQ-R-S. Transcriptional fusion data reported here support the results of recent molecular analysis of the 5Ј ends of prgB transcripts which indicated that prgB transcription o… Show more

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Cited by 43 publications
(36 citation statements)
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“…Figure 2 shows that when RNA from strain OG1RF(pCF10) was probed with the entire prgQ region, the primary transcripts detected in RNA from uninduced cells were the 400 nt QS and a 300 nt fragment. The 300 nt fragment may be the most stable degradation product, and is discussed further in the accompanying paper (Bensing and Dunny, 1997). A faint signal at 1.4 kb was also evident, and corresponds to transcripts spanning the prgQ-R-S region.…”
Section: Resultsmentioning
confidence: 79%
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“…Figure 2 shows that when RNA from strain OG1RF(pCF10) was probed with the entire prgQ region, the primary transcripts detected in RNA from uninduced cells were the 400 nt QS and a 300 nt fragment. The 300 nt fragment may be the most stable degradation product, and is discussed further in the accompanying paper (Bensing and Dunny, 1997). A faint signal at 1.4 kb was also evident, and corresponds to transcripts spanning the prgQ-R-S region.…”
Section: Resultsmentioning
confidence: 79%
“…Inhibition of downstream ORF translation is not entirely due to cis-action of iCF10, but may be due to action of other negative control molecules, such as PrgX. Second, pheromone cCF10 may interact directly with ribosomal proteins (Bensing and Dunny, 1997;Leonard et al, 1996). Third, evidence suggests that QL RNA associates with ribosomes to affect translation of the prgB mRNA (Bensing and Dunny, 1997).…”
Section: Discussionmentioning
confidence: 99%
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