Phosphate repression of phage protein synthesis during infection by choleraphage φ149

Ray, Prabir; Sengupta, Anuradha; Das, Jyotirmoy

doi:10.1016/0042-6822(84)90252-6

Cited by 17 publications

(43 citation statements)

References 17 publications

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“…cholerae strain 569B and its detergent-resistant mutant, lpa-1 (Paul et al, 1990) were grown in complex media with shaking at 37 "C as described previously (Lohia et al, 1984). A low phosphate medium was used for the induction of alkaline phosphatase (Roy et al, 1982). Escherichia coli C-600 was grown with shaking at 37 "C in Luria broth.…”

Section: Methodsmentioning

confidence: 99%

“…Since [14C]acetate could also label phospholipids, the radioactive LPS was freed from phospholipids by repeated extraction with chloroform/methanol(2 : 1, v/v) till no further counts were extractable. Finally, the labelled LPS was solubilized in Laemmli sample buffer and analysed by 14% (w/v) SDS-PAGE and fluorography (Ray et al, 1984). Phospholipids were extracted from whole cells and isolated membranes by chloroform/methanol(2 : 1, v/v) and quantitatively analysed after their separation by TLC as described previously (Lohia et al, 1985).…”

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

“…Protein was estimated by the method of Markwell et al (1978). Alkaline phosphatase and cyclic phosphodiesterase were assayed by standard methods using p-nitrophenyl phosphate and bis-p-nitrophenyl phosphate as substrates respectively (Roy et al, 1982;Neu & Heppel, 1965). Enzyme activity in cell pellet was assayed after sonication for 1 min at 4 "C.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

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Presence of exposed phospholipids in the outer membrane of Vibrio cholerae

Paul

Chaudhuri²,

Chatterjee³

et al. 1992

Journal of General Microbiology

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Vibrio choferae 569B was found to be highly sensitive to a wide range of chemicals, particularly hydrophobic compounds and neutral and anionic detergents. The phospholipid profile of the outer membrane was similar to that reported for other Gram-negative bacteria. The lipopolysaccharide (LPS) contained 0-antigenic sugars and exhibited heterogeneity. In addition, the LPS moiety was characterized by a relatively low negative charge. Analysis by topological probes revealed the presence of a significant amount of exposed phospholipids in the outer membrane. The reduced negative charge of LPS molecules and the exposed phospholipids present in the outer membrane could be important in the increased permeation of exogenous compounds in V. choferae.

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Section: Methodsmentioning

confidence: 99%

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

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Presence of exposed phospholipids in the outer membrane of Vibrio cholerae

Paul

Chaudhuri²,

Chatterjee³

et al. 1992

Journal of General Microbiology

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“…The growth of this phage is extremely sensitive to the concentration of phosphate ions, and no phage growth occurs in medium containing more than * Corresponding author. 0.1% phosphate (31). After infection under high-phosphate conditions, concatemeric DNA structures are not formed, although synthesis of monomeric molecules is unaffected (11).…”

mentioning

confidence: 99%

“…The DNA molecules are a limited set of circular permutation of the phage genome and have several single-strand interruptions along their length that are repairable by DNA ligase (36). The phage DNA codes for 26 early and 23 late proteins (31). The intracellular replication of phage 4149 DNA involves a concatemeric DNA replicative intermediate serving as the substrate for the synthesis of mature phage DNA, which is eventually packaged by a headful mechanism (11).…”

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confidence: 99%

A 14-kilodalton inner membrane protein of Vibrio cholerae biotype e1 tor confers resistance to group IV choleraphage infection to classical vibrios

1992

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Choleraphage +149 differentiates the two biotypes, classical and el tor, of Vibrio cholerae. This phage cannot replicate in V. cholerae biotype el tor cells because the concatemeric DNA intermediates produced are unstable and cannot be chased to mature phage DNA. A V. cholerae biotype el tor gene coding for a 14,000-Da inner membrane protein which destabilizes the concatemeric DNA intermediates by hindering their binding to the cell membrane has been identified. Presumably, a 22,000-Da V. cholerae biotype el tor protein might also have a role in conferring phage +149 resistance to cells belonging to the biotype el tor. A nucleotide sequence homologous to the 1.2-kb V. cholerae biotype el tor DNA coding for both the 14,000-and 22,000-Da proteins is present in all strains of classical vibrios but is not transcribed. The nucleotide sequence of the gene coding for the 14,000-Da protein has been determined.Vibrio cholerae strains of serotype 01 comprise two different biotypes, classical and el tor. Of the seven pandemics of cholera recorded in recent times, V. cholerae biotype el tor has been identified as the causative agent of the seventh pandemic, while the previous ones were due to the classical strains. Recently, there has been a resurgence of classical vibrios in certain areas of endemicity (33). In spite of broad similarities in the nature of infection by the classical and el tor vibrios, important differences exist in the epidemiology of el tor compared with that of classical vibrios. The ratio of asymptomatic infection to clinical illness is greater with the el tor biotype than with the classical vibrios (2), and the el tor vibrios survive longer under unfavorable conditions. Both the classical and the el tor strains of V. cholerae react with V. cholerae antisera, and taxonomic studies have shown that these two biotypes are the same species (12). They are isogenic, and tests to distinguish them often give ambiguous results (16). One of the most reliable criteria differentiating between these two biotypes is their susceptibility to group IV choleraphages. This group of choleraphages can infect and lyse all strains of classical vibrios but none of the el tor biotype (24). Phage 4149 adsorbs irreversibly to the biotype el tor cells, and 50% of the injected phage DNA binds to the cell membrane. Synthesis of monomeric phage DNA continued, similar to that observed for the permissive host. However, the concatemeric DNA intermediates produced were unstable and could not be chased to mature phage DNA (9). Although most of the early proteins are made, only some of the late proteins were transiently synthesized for infection in el tor cells. Formation and stabilization of concatemeric DNA structures essential for the synthesis of mature phage DNA and their packaging into phage heads are not hindered in V. cholerae biotype el tor cells for the replication of choleraphage 4138 belonging to serological group II. This phage also contains a linear double-stranded circularly permuted DNA molecule and can replicate in both V....

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Cloning and expression in Escherichia coli of a recA-like gene from Vibrio cholerae

1986

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A library containing more than 80% of the Vibrio cholerae genome was constructed by cloning BamH1 restriction fragments into pBR322. Using interspecific complementation of an Escherichia coli recA mutant with plasmids containing the gene bank of V. cholerae, a recA-like gene was identified. The recombinant plasmid, designated as pDP145, contained a 1.45 kb segment of V. cholerae DNA which codes for a protein of molecular weight 39,000. The product of this gene confers methyl methane sulphonate resistance on the E. coli recA mutant, suppresses its ultraviolet (UV) light sensitive phenotype and has proteolytic activity on the phage lambda repressor. Induction of a 39,000 dalton protein in UV-irradiated V. cholerae cells was demonstrated.

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Phosphate repression of phage protein synthesis during infection by choleraphage φ149

Cited by 17 publications

References 17 publications

Presence of exposed phospholipids in the outer membrane of Vibrio cholerae

Presence of exposed phospholipids in the outer membrane of Vibrio cholerae

A 14-kilodalton inner membrane protein of Vibrio cholerae biotype e1 tor confers resistance to group IV choleraphage infection to classical vibrios

Cloning and expression in Escherichia coli of a recA-like gene from Vibrio cholerae

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