Phosphatidic Acid Production in the Processing of Cabbage Leaves

Urikura, Mai; Morishige, Jun‐ichi; Tanaka, Tamotsu; Satouchi, Kiyoshi

doi:10.1021/jf303515z

Cited by 10 publications

(6 citation statements)

References 28 publications

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“…Cabbage is known to contain abundant PLD activity [17]. The PLD activity converts endogenous phospholipids to phosphatidic acid when raw leaves are homogenized [18]. In fact, cabbage lipids extracted from raw leaves contain much more phosphatidic acid than lipids from boiled leaves ( Fig.…”

Section: Formation Of Pc1p In Cabbage Homogenatesmentioning

confidence: 99%

Identification of a sphingolipid‐specific phospholipase D activity associated with the generation of phytoceramide‐1‐phosphate in cabbage leaves

et al. 2013

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The structure and biosynthetic route for an unidentified lipid (lipid X) detected by TLC of cabbage (Brassica oleracea) lipids was determined. Lipid X is a phospholipid that is resistant to mild alkali and detectable by MALDI-TOF MS as an adduct with Phos-tag, a phosphate-capture zinc complex. Various α-hydroxy fatty acids (16:0, 22:0, 24:0 and 24:1) were detected by GC-MS of fatty acid methyl esters prepared from lipid X. The deacyl derivative of lipid X was determined to be 4-hydroxysphingenine (dehydrophytosphingosine)-1-phosphate by MALDI-TOF MS with Phos-tag. From these results, lipid X was determined to be phytoceramide-1-phosphate (PC1P) with an α-hydroxy fatty acid. When cabbage homogenates were incubated, PC1P was formed, with a concomitant decrease in the amount of glycosylinositol phosphoceramide (GIPC). The formation of PC1P from GIPC was confirmed by treatment of purified cabbage GIPC with a membrane fraction of cabbage homogenates. Using a partially purified enzyme fraction, we found that the enzyme hydrolyzes GIPC specifically, but not glycerophospholipids and sphingomyelin. Arabidopsis thaliana also had this enzyme activity. From these results, we conclude that a previously uncharacterized phospholipase D activity that specifically hydrolyzes GIPC produces PC1P in brassicaceous plants.

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Section: Formation Of Pc1p In Cabbage Homogenatesmentioning

confidence: 99%

Identification of a sphingolipid‐specific phospholipase D activity associated with the generation of phytoceramide‐1‐phosphate in cabbage leaves

et al. 2013

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“…PA Content in Boiled and Non-boiled Cereals. Previously, we showed that cutting or mashing of raw cabbage leaves induced PA formation in the leaves, 20 which was caused by hydrolysis of endogenous phospholipids, including PC by activated phospholipase D (PLD) during tissue destruction. Consistent with our previous findings, levels of PA prepared from boiled cabbage leaves, radish roots, and carrot roots were low relative to those prepared from raw ones.…”

Section: ■ Resultsmentioning

confidence: 99%

Concentrated Phosphatidic Acid in Cereal Brans as Potential Protective Agents against Indomethacin-Induced Stomach Ulcer

Afroz

Ikoma

Yagi

et al. 2016

J. Agric. Food Chem.

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One of complications associated with long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) is peptic ulcer. Recently, we found that orally administered phosphatidic acid (PA) ameliorated aspirin-induced stomach lesions in mice. In this study, we identified PA-rich food sources and examined the effects of the food materials on indomethacin-induced stomach ulcer. Among examined, buckwheat (Fagopyrum esculentum) bran contained the highest level of PA (188 mg/100 g). PA was the richest phospholipid (25%) in the lipid fraction of the buckwheat bran. Administration of the lipid extracts of buckwheat bran significantly ameliorated indomethacin-induced stomach lesions in mice. In contrast, wheat (Triticum durum) bran lipids (PA, 4%) and soybean (Glycine max) lipids (PA, 3%) were not associated with ameliorative effects. These results indicated that PA-rich lipids can be used as an effective supplement for prevention of NSAID-induced stomach ulcer.

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“…Laser light scattering and multi size coulter counter other than instrumental methods, which can be used for the characterization of size, shape and morphology of the microspheres. 50…”

Section: Particle Size and Shapementioning

confidence: 99%

Review on Microspheres as Drug Carriers for Controlled Drug Delivery

Sahu

Jadon

Jain³

et al. 2021

Int J Med Sci Pharm Res

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Microspheres are organic or inorganic spherical particles with a diameter of 1–1000 μm. These materials can encapsulate drugs or bioactive molecules and release them in a controlled way. Biodegradable polymers are frequently used for the development of microsphere matrixes such as polylactic acid and copolymer of lactic acid and glycolic acid. Apart from them, there is an extensive range of microspheres prepared from albumin, albumin dextran sulfate, and fibrinogen. Administration of medication via micro particulate systems is advantageous because microspheres can be ingested or injected; they can be tailored for desired release profiles and used for site-specific delivery of drugs and in some cases can even provide organ-targeted release. Microspheres in drug delivery are used for targeted as well as prolonged drug release in the diseased area. It also protects the unstable or pH-sensitive drugs before and after the administration. In this review we have discussed about advantages, disadvantages, methods of preparations and evaluation and types of microspheres. Keywords: Microsphere, Biodegradable polymers, Polymer Microsphere, Drug Release

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Phosphatidic Acid Production in the Processing of Cabbage Leaves

Cited by 10 publications

References 28 publications

Identification of a sphingolipid‐specific phospholipase D activity associated with the generation of phytoceramide‐1‐phosphate in cabbage leaves

Identification of a sphingolipid‐specific phospholipase D activity associated with the generation of phytoceramide‐1‐phosphate in cabbage leaves

Concentrated Phosphatidic Acid in Cereal Brans as Potential Protective Agents against Indomethacin-Induced Stomach Ulcer

Review on Microspheres as Drug Carriers for Controlled Drug Delivery

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